Rabbit Recombinant Monoclonal Stanniocalcin 2/STC-2 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 2 publications.
pH: 7.2 - 7.4
Constituents: PBS
IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
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Human | Tested | Expected | Tested | Tested | Tested |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
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Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Has an anti-hypocalcemic action on calcium and phosphate homeostasis.
Stanniocalcin-2, STC-2, Stanniocalcin-related protein, STC-related protein, STCRP, STC2
Rabbit Recombinant Monoclonal Stanniocalcin 2/STC-2 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 2 publications.
pH: 7.2 - 7.4
Constituents: PBS
ab261915 is the carrier-free version of Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Stanniocalcin 2 (STC-2) is a glycoprotein hormone known for regulating calcium and phosphate homeostasis. It has other names like STC-1-related protein and STC2. The protein has a mass of approximately 33 kDa. STC-2 expresses in various tissues with high levels in the liver kidney and thyroid gland. Its expression suggests an important role in maintaining mineral balance in these tissues.
STC-2 influences metabolic and inflammatory processes by modulating cellular responses to calcium and phosphate. It operates partly by binding to receptors on cell surfaces impacting cell proliferation and survival. While not part of a known complex STC-2 interacts with signaling molecules involved in bone metabolism and endocrine function serving as a modulator of mineralized tissue homeostasis.
In mineral homeostasis STC-2 interacts within pathways involving calcium and phosphate regulation. Its interactions include overlap with the PTH (parathyroid hormone) and vitamin D pathways each critical for calcium and bone metabolism. STC-2 shows connections with proteins like FGF23 (fibroblast growth factor 23) that also participate in these regulatory networks impacting how cells manage mineral concentrations.
Research indicates STC-2's involvement in conditions such as chronic kidney disease and some cancers. Its expression levels correlate with abnormal mineral metabolism in kidney disease where it may interact with proteins like Klotho a known regulator of phosphate transport. In oncology overexpression of STC-2 associates with cancer progression potentially linking to altered signaling pathways in tumor cells.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
STC2 was immunoprecipitated from 0.35 mg T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate with Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate 10ug
Lane 2: Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610 IP in T-47D whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610 in T-47D whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610).
All lanes: Immunoprecipitation - Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] (Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610)
Predicted band size: 33 kDa
Observed band size: 33-35 kDa
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling STC2 with Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610).
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cells labelling STC2 with Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610).
Immunohistochemical analysis of paraffin-embedded Human breast cancer (A) and its adjacent noncancerous breast tissue (B) tissue labeling STC2 with Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610 at 1/ 200 dilution (2.29ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Strong cytoplasmic staining in human breast cancer tissue (A) while weak staining in its adjacent noncancerous breast tissue (B) (PMID: 18492817) is observed. The section was incubated with Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610).
Immunohistochemical analysis of paraffin-embedded Human lung cancer (A) and its adjacent noncancerous lung tissue (B) tissue labeling STC2 with Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610 at 1/ 200 dilution (2.29ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Strong cytoplasmic staining in human lung cancer tissue (A) while weak staining in its adjacent noncancerous lung tissue (B) (PMID: 25463045) is observed. The section was incubated with Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610).
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling STC2 with Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610 at 1/50 (9 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610).
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cells labelling STC2 with Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610 at 1/50 (9 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in T-47D cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610 anti-STC2 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Stanniocalcin 2/STC-2 antibody [EPR22866-10] ab255610).
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