Rabbit Recombinant Monoclonal Stanniocalcin 2/STC-2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-Stanniocalcin 2/STC-2 antibody [EPR25620-176] - BSA and Azide free (Detector) (AB303622), expandable thumbnail](https://content.abcam.com/products/images/stanniocalcin-2-stc-2-antibody-epr25620-176-bsa-and-azide-free-detector-ab303622--sandwich-elisa-img402416.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Stanniocalcin 2/STC-2 antibody [EPR25620-176] - BSA and Azide free (Detector) (AB303622)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Has an anti-hypocalcemic action on calcium and phosphate homeostasis.
Alternative names
Stanniocalcin-2, STC-2, Stanniocalcin-related protein, STC-related protein, STCRP, STC2
Recommended products
Rabbit Recombinant Monoclonal Stanniocalcin 2/STC-2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Alternative names
Stanniocalcin-2, STC-2, Stanniocalcin-related protein, STC-related protein, STCRP, STC2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR25620-176
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab303622 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify human Stanniocalcin 2/STC-2.
The recommended pair for sandwich ELISA is:
Capture: Anti-Stanniocalcin 2/STC-2 antibody [EPR25620-118] - BSA and Azide free (Capture) ab303621, human Stanniocalcin 2/STC-2 Capture Antibody (unconjugated)
Detector: ab303622, human Stanniocalcin 2/STC-2 Detector Antibody (unconjugated)
The reference range value is 15.63-2000 pg/ml
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- Activity summary
Stanniocalcin 2 (STC-2) is a glycoprotein hormone known for regulating calcium and phosphate homeostasis. It has other names like STC-1-related protein and STC2. The protein has a mass of approximately 33 kDa. STC-2 expresses in various tissues with high levels in the liver kidney and thyroid gland. Its expression suggests an important role in maintaining mineral balance in these tissues.
- Biological function summary
STC-2 influences metabolic and inflammatory processes by modulating cellular responses to calcium and phosphate. It operates partly by binding to receptors on cell surfaces impacting cell proliferation and survival. While not part of a known complex STC-2 interacts with signaling molecules involved in bone metabolism and endocrine function serving as a modulator of mineralized tissue homeostasis.
- Pathways
In mineral homeostasis STC-2 interacts within pathways involving calcium and phosphate regulation. Its interactions include overlap with the PTH (parathyroid hormone) and vitamin D pathways each critical for calcium and bone metabolism. STC-2 shows connections with proteins like FGF23 (fibroblast growth factor 23) that also participate in these regulatory networks impacting how cells manage mineral concentrations.
- Associated diseases and disorders
Research indicates STC-2's involvement in conditions such as chronic kidney disease and some cancers. Its expression levels correlate with abnormal mineral metabolism in kidney disease where it may interact with proteins like Klotho a known regulator of phosphate transport. In oncology overexpression of STC-2 associates with cancer progression potentially linking to altered signaling pathways in tumor cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-Stanniocalcin 2/STC-2 antibody [EPR25620-176] - BSA and Azide free (Detector) (ab303622), expandable thumbnail](https://content.abcam.com/products/images/stanniocalcin-2-stc-2-antibody-epr25620-176-bsa-and-azide-free-detector-ab303622--sandwich-elisa-img402416.jpg "Sandwich ELISA - Anti-Stanniocalcin 2/STC-2 antibody [EPR25620-176] - BSA and Azide free (Detector) (ab303622)")
Sandwich ELISA - Anti-Stanniocalcin 2/STC-2 antibody [EPR25620-176] - BSA and Azide free (Detector) (ab303622)
Sandwich ELISA of Human Stanniocalcin 2/STC-2 Antibody Pair - BSA and Azide free ab303620 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com