AB20920

Anti-Staphylococcus aureus antibody

Name: Anti-Staphylococcus aureus antibody (ab20920) | Abcam
Brand: Abcam Limited
SKU: AB20920
Price: 400 USD
Availability: InStock
Rating: 4 (8 reviews)

(8 Reviews)

(68 Publications)

Anti-Staphylococcus aureus antibody (ab20920) is a rabbit polyclonal antibody detecting Staphylococcus aureus in IHC-Fr, ICC/IF. Suitable for Staphylococcus aureus.

- Over 40 publications
- Trusted since 2005

View Alternative Names

IgG binding protein A, Immunoglobulin G binding protein A

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Staphylococcus aureus antibody (AB20920)

ICC/IF

AbReview27826****

Immunocytochemistry/ Immunofluorescence - Anti-Staphylococcus aureus antibody (AB20920)

ab20920 staining Staphylococcus aureus in human keratinocytes by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in methanol, permeabilized using 0.1% saponin/ PBS, blocked with 4% BSA for 30 minutes at 25°C and then incubated with ab20920 at 10μg/ml for 14 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.

Red : cadherin
Blue : DAPI
Green : anti-Staphylococcus aureus (ab20920)

Image courtesy of an anonymous Abreview.

Immunohistochemistry (Frozen sections) - Anti-Staphylococcus aureus antibody (AB20920)

IHC-Fr

AbReview9540****

Immunohistochemistry (Frozen sections) - Anti-Staphylococcus aureus antibody (AB20920)

ab20920 staining an abscess in rat calf tissue prepared as a 20 μm cryoslice. Tissue was fixed in paraformaldehyde, permeabilised with 0,5% Trition X100 in PBS, and blocked with 10% serum plus 1% BSA for 1 hour at room temperature. The primary antibody was diluted 1/500 with PBS plus 3-5% Serum, 0,5% Trition X100 and incubated for 24 hours at 20°C. An Alexa Fluor® 568 goat anti-rabbit was used as the secondary

This image is courtesy of an Abreview submitted by Dr Dagmar Salber

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Staphylococcus aureus

Applications

IHC-Fr, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Soluble and structural antigens of the whole bacterium. Antiserum is not absorbed for related microorganisms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Staphylococcus aureus": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p>Use with acetone fixed tissues.</p>" } } }

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Product details

What is this antibody validated in?
Anti-Staphylococcus aureus antibody (ab20920) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Staphylococcus aureus samples.

Trusted by the scientific community
Anti-Staphylococcus aureus (ab20920) was first used in a scientific publication in 2005 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists
Anti-Staphylococcus aureus (ab20920) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Staphylococcus aureus also known as "aureus" or "staph" is a gram-positive bacterium with a molecular mass ranging around 2.8 million daltons. It is found mainly in the nasal passages and on the skin of humans. This bacterium has a thick peptidoglycan layer making it resistant to certain environmental pressures. The structure of its proteins like Protein A plays an important role in immune evasion. Staphylococcus aureus also expresses a variety of toxins and enzymes that aid in its survival and pathogenicity.

Biological function summary

Staphylococcus aureus acts as an opportunistic pathogen that can cause infections when the host's immune system is weakened. It is not part of a specific protein complex but utilizes a range of virulence factors to establish infections. The bacterium produces biofilms which provide a protective environment against host defenses and antimicrobial agents. Its ability to quickly adapt to different environments makes it a major cause of both nosocomial and community-acquired infections.

Pathways

The metabolic activities of Staphylococcus aureus are involved in two significant pathways: the peptidoglycan biosynthesis and the toxin production pathway. Peptidoglycan biosynthesis is essential for cell wall integrity while toxin production pathways lead to the synthesis of critical virulence factors like alpha-hemolysin. Key proteins involved in these pathways include PBP2a which is significant for methicillin resistance and hemolysins that damage host tissues.

Staphylococcus aureus is connected to skin infections and pneumonia. The bacterium is notorious for causing conditions such as MRSA (methicillin-resistant Staphylococcus aureus) infections which are challenging to treat due to antibiotic resistance. Protein A interaction with the Fc region of immunoglobulin contributes to the evasion of immune responses and chronic infection persistence. These properties make staph infections a significant concern in both hospital and community settings.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Staphylococcus aureus

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Publications (68)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:9326-9343 PubMed40963910

2025

Triple-targeting nanosystems with synergistic effects on iron Trojan horse, fluoroquinolone antibiotics, and photodynamic therapy specifically kill intracellular MRSA.

Applications

Unspecified application

Species

Unspecified reactive species

Kuiyu Meng,Liwen Yuan,Lulu Feng,Yaoyao Zhang,Hao Wu,Jie Zhang,Mubbashar Abbas,Wei Qu,Dongmei Chen,Shuyu Xie

PLoS pathogens 21:e1013126 PubMed40294039

2025

Astrocyte-derived complement C3 facilitated microglial phagocytosis of synapses in Staphylococcus aureus-associated neurocognitive deficits.

Applications

Unspecified application

Species

Unspecified reactive species

Haifang Zhang,Qiyuan Jin,Jijie Li,Jiali Wang,Mengqi Li,Qiao Yin,Qi Li,Yuwan Qi,Lingling Feng,Liang Shen,Yuan Qin,Qifei Cong

PloS one 20:e0318955 PubMed40193365

2025

A novel approach to studying infective endocarditis: Ultrasound-guided wire injury and bacterial challenge in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Benedikt Bartsch,Ansgar Ackerschott,Muntadher Al Zaidi,Raul Nicolas Jamin,Mariam Louis Fathy Nazir,Moritz Altrogge,Lars Fester,Jessica Lambertz,Mark Coburn,Georg Nickenig,Marijo Parcina,Sebastian Zimmer,Christina Katharina Weisheit

Antibiotics (Basel, Switzerland) 14: PubMed40149081

2025

Methicillin-Resistant T144: A Hypervirulent Model Strain for Infection Models.

Applications

Unspecified application

Species

Unspecified reactive species

Changsi Mao,Yuan Liu,Meirong Song,Jianzhong Shen,Kui Zhu

Frontiers in cellular and infection microbiology 15:1543186 PubMed40061451

2025

utilizes vimentin to internalize human keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Kyoungok Jang,Hangeun Kim,Dobin Choi,Soojin Jang,Dae-Kyun Chung

PLoS pathogens 21:e1012896 PubMed39869636

2025

Staphylococcus aureus blocks host autophagy through circSyk/miR-5106/Sik3 axis to promote progression of bone infection.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihao Chen,Qiyuan Jin,Jinqi Zhong,Zonggang Xie,Qi Chen,Liubing Li,Jijie Li,Chenhao Zhao,Junfeng Wang,Xiaoying Tang,Mingxiao Han,Ru Li,Ziyuan Li,Zelei Tong,Min Wang,Hong Du,Haifang Zhang

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 33:e13253 PubMed39823159

2025

Bacteria in hypertrophic scars promote scar formation through HSBP1-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Yuan,Jiarong Yu,Jiaoyun Dong,Zhigang Mao,Xiqiao Wang

Microorganisms 12: PubMed39338569

2024

Mapping at Early and Late Stages of Infection in a Clinically Representative Hip Prosthetic Joint Infection Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Mariam Taha,Abdullah AlDuwaisan,Manijeh Daneshmand,Mazen M Ibrahim,Jonathan Bourget-Murray,George Grammatopoulos,Simon Garceau,Hesham Abdelbary

Journal of nanobiotechnology 22:548 PubMed39238028

2024

A nanoscale visual exploration of the pathogenic effects of bacterial extracellular vesicles on host cells.

Applications

Unspecified application

Species

Unspecified reactive species

Minjae Kang,Min Jeong Kim,Dokyung Jeong,Hyung-Jun Lim,Ga-Eun Go,Uidon Jeong,Eunyoung Moon,Hee-Seok Kweon,Nae-Gyu Kang,Seung Jin Hwang,Sung Hun Youn,Bo Kyoung Hwang,Doory Kim

Infection and immunity 92:e0030424 PubMed39150268

2024

RSV enhances bacterial growth in the lung.

Applications

Unspecified application

Species

Unspecified reactive species

Helen E Rich,Simran Bhutia,Francina Gonzales de Los Santos,Gabrielle P Entrup,Helen I Warheit-Niemi,Stephen J Gurczynski,Monica Bame,Michael T Douglas,Susan B Morris,Rachel L Zemans,Nicholas W Lukacs,Bethany B Moore

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Staphylococcus aureus antibody

Immunocytochemistry/ Immunofluorescence - Anti-Staphylococcus aureus antibody (AB20920)

Immunohistochemistry (Frozen sections) - Anti-Staphylococcus aureus antibody (AB20920)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (68)

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