JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB190337

Anti-Staphylococcus aureus Enterotoxins A + B + TSST-1 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal ETXA antibody. Suitable for ELISA, WB, sELISA and reacts with Staphylococcus aureus samples. Cited in 1 publication. Immunogen corresponding to Chemical / Small Molecule corresponding to entA.

View Alternative Names

Enterotoxin type A, SEA, entA

2 Images
ELISA - Anti-Staphylococcus aureus Enterotoxins A + B + TSST-1 antibody (AB190337)
  • ELISA

Supplier Data

ELISA - Anti-Staphylococcus aureus Enterotoxins A + B + TSST-1 antibody (AB190337)

Various Staphylococcal toxins were coated to a plate at a concentration of 1 μg/mL in PBS. ab190337 was serially diluted semi-log from 2 μg/mL and incubated with the toxin coated plate. Washed plates were detected with anti-rabbit HRP conjugate and TMB substrate. OD650 was reported above.

Western blot - Anti-Staphylococcus aureus Enterotoxins A + B + TSST-1 antibody (AB190337)
  • WB

Supplier Data

Western blot - Anti-Staphylococcus aureus Enterotoxins A + B + TSST-1 antibody (AB190337)

Bands visualized using TMB membrane substrate.

All lanes:

Western blot - Anti-Staphylococcus aureus Enterotoxins A + B + TSST-1 antibody (ab190337) at 1 µg/mL

Lane 1:

Enterotoxin B (SEB) at 0.05 µg

Lane 2:

Enterotoxin A (SEA) at 0.05 µg

Lane 3:

Enterotoxin C (SEC-1) at 0.05 µg

Lane 4:

Toxic Shock Syndrome Toxin (TSST-1) at 0.05 µg

Lane 5:

24 hour culture supernatant of an SEB producing Staphylocccus aureus strain at 15 µL

Lane 6:

Media without culture growth at 15 µL

Lane 7:

LukS-PV at 0.05 µg

Lane 8:

LukF-PV at 0.05 µg

Secondary

All lanes:

Anti-rabbit HRP conjugate

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Staphylococcus aureus

Applications

WB, ELISA, sELISA

applications

Immunogen

This product was produced with the following immunogens:

Chemical / Small Molecule corresponding to entB.

Chemical / Small Molecule corresponding to tst.

Specificity

ab190337 was designed to broadly react with a number of toxins produced by Staphylococcus aureus strains. Those confirmed include SEA, SEB, SEC, TSST-1. This antibody does not detect alpha-hemolysin, nor LukS and LukF.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Staphylococcus aureus": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p><a href='/en-us/products/primary-antibodies/entb-antibody-3d10-ab190465'>ab190465</a> could be used as capture antibody.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Staphylococcus aureus Enterotoxins A B and TSST-1 are superantigens produced by Staphylococcus aureus bacteria with masses around 27 kDa for SEA and 29 kDa for SEB. These toxins disrupt normal immune function by binding to major histocompatibility complex (MHC) class II molecules and T-cell receptors without antigen specificity. They are mainly expressed by strains of Staphylococcus aureus located on the skin and mucosal surfaces as well as within wounds and the gastrointestinal tract.
Biological function summary

Staphylococcus aureus enterotoxins activate a large number of T-cells causing massive cytokine release also known as a cytokine storm. SEA SEB and TSST-1 are standalone toxins not requiring a complex to function. However they target the interaction between APCs (Antigen Presenting Cells) and T-cells which can lead to excessive immune response and tissue damage.

Pathways

These enterotoxins induce signals in the immune system affecting pathways like the T-cell receptor signaling pathway. They bind to proteins such as the MHC class II molecules and T-cell receptors thoughtlessly leading to a large spectrum of immune responses. These interactions with proteins create an unbalanced stimulation in the immune system contributing to pathogenesis.

The toxins can contribute to conditions such as toxic shock syndrome and staphylococcal food poisoning. TSST-1 specifically relates to toxic shock syndrome by creating a system-wide response from its superantigen activity. SEA and SEB can also mediate connections to other diseases of overactive immune responses due to their similar mechanisms of T-cell stimulation.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules (PubMed : 12467569, PubMed : 2658055). In turn, waves of cellular activation, cytokine production, and migration into the lung tissue and airways occur via alphabeta T-cells (PubMed : 23321986). Causes also the intoxication staphylococcal food poisoning syndrome. The illness is characterized by high fever, hypotension, diarrhea, shock, and in some cases death (Probable).
See full target information entA

Additional targets

tst,entB
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 9:2702 PubMed30006572

2018

ATP synthase F subunits recruited to centromeres by CENP-A are required for male meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Caitríona M Collins,Beatrice Malacrida,Colin Burke,Patrick A Kiely,Elaine M Dunleavy
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB243103

Anti-Staphylococcus Enterotoxin A antibody [SA01]

Western blot - Anti-Staphylococcus Enterotoxin A antibody [SA01] (AB243103)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB52953

Recombinant Staphylococcus aureus Protein A

Size Exclusion Chromatography - Recombinant Staphylococcus aureus Protein A (AB52953)

  • 0
  • 0
Primary Antibodies

AB69236

Anti-Serratia marcescens antibody [B/N4N]

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com