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AB243103

Anti-Staphylococcus Enterotoxin A antibody [SA01]

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Mouse Monoclonal ETXA antibody. Suitable for ELISA, WB and reacts with Staphylococcus aureus, Purified native protein - Staphylococcus aureus samples.

View Alternative Names

Enterotoxin type A, SEA, entA

1 Images
Western blot - Anti-Staphylococcus Enterotoxin A antibody [SA01] (AB243103)
  • WB

Supplier Data

Western blot - Anti-Staphylococcus Enterotoxin A antibody [SA01] (AB243103)

All lanes:

Western blot - Anti-Staphylococcus Enterotoxin A antibody [SA01] (ab243103) at 1/500 dilution

Lane 1:

Culture medium of Staphylococcus aureus

Lane 2:

Purified Staphylococcus aureus Enterotoxin A protein

Lane 3:

Purified Staphylococcus aureus Enterotoxin B protein

Predicted band size: 30 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SA01

Isotype

IgG2a

Carrier free

No

Reacts with

Staphylococcus aureus

Applications

WB, ELISA

applications

Specificity

Reacts with enterotoxin A and B of Stapylococcus aureus. Reactivity with other types of the enterotoxins has not been tested.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Purified native protein - Staphylococcus aureus": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Staphylococcus aureus": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Affinity purified with Protein A/G Mix.
Storage buffer
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Staphylococcus Enterotoxin A (SEA) also known simply as Enterotoxin A is one of the key virulence factors produced by the bacterium Staphylococcus aureus. This protein is classified as an enterotoxin and has a molecular mass of approximately 27 kDa. SEA is endogenously expressed by Staphylococcus aureus and it is known to exert its effects in the human gastrointestinal tract by binding to major histocompatibility complex (MHC) class II molecules thereby activating T-cells.
Biological function summary

The function of SEA involves triggering a strong immune response through its role as a superantigen. Enterotoxins such as SEA can bypass the conventional antigen presentation pathway leading to massive cytokine release. This protein can provoke a large-scale activation of T-cells not through specific antigen binding but rather through cross-linking of MHC class II molecules and T-cell receptors. SEA does not typically form complexes with other proteins as its main mechanism of action relies on interaction with cell surface receptors.

Pathways

SEA integrates into the immune response pathway by inducing excessive cytokine production contributing to inflammation. It plays a notable role in the cytokine signaling pathway causing unregulated production of inflammatory cytokines. This interferes with normal immune system function leading to toxic shock syndrome in severe cases. SEA also has a connection with other enterotoxins produced by Staphylococcus aureus such as Enterotoxin B through its role in stimulating immune responses.

SEA is associated with food poisoning and toxic shock syndrome. Food poisoning arises from the ingestion of food contaminated with SEA leading to nausea vomiting and diarrhea. Toxic shock syndrome occurs when SEA enters the bloodstream causing systemic inflammation and acute symptoms. SEA has an indirect relationship with other staphylococcal toxins as they share similar pathways in disease progression including Enterotoxin B which also contributes to toxic shock syndrome.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules (PubMed : 12467569, PubMed : 2658055). In turn, waves of cellular activation, cytokine production, and migration into the lung tissue and airways occur via alphabeta T-cells (PubMed : 23321986). Causes also the intoxication staphylococcal food poisoning syndrome. The illness is characterized by high fever, hypotension, diarrhea, shock, and in some cases death (Probable).
See full target information entA
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