AB133657

Anti-StAR antibody [EP7639]

Name: Anti-StAR antibody [EP7639] (ab133657) | Abcam
Brand: Abcam Limited
SKU: AB133657
Price: 325 USD
Availability: InStock
Rating: 4 (3 reviews)

RabMAb
Recombinant
What is this?

(3 Reviews)

(12 Publications)

Rabbit Recombinant Monoclonal STAR antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 12 publications.

View Alternative Names

STARD1, STAR, StAR, START domain-containing protein 1, StARD1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (AB133657)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (AB133657)

Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labelling StAR with ab133657 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-StAR antibody [EP7639] (AB133657)

All lanes:

Western blot - Anti-StAR antibody [EP7639] (ab133657) at 1/10000 dilution

All lanes:

Human adrenal gland lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 32 kDa

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IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (AB133657)

ab133657 showing positive staining in Adrenocortical carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (AB133657)

ab133657 showing positive staining in Sertoli-Leydig cell tumor tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (AB133657)

ab133657 showing negative staining in Skeletal muscle tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (AB133657)

ab133657 showing positive staining in Normal testis tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

CiteAb

Western blot - Anti-StAR antibody [EP7639] (AB133657)

StAR western blot using anti-StAR antibody [EP7639] ab133657. Publication image and figure legend from Chen, Y., Li, C., et al., 2019, J Cell Mol Med, PubMed 31293077.

ab133657 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab133657 please see the product overview.

Identification of Leydig‐like cells derived from induced pluripotent stem cells (ADSC‐LCs). A, Representative flow cytometry histograms for CYP17A1, HSD3B1, HSD11B1 and OCT4 in ADSCs, LCs and ADSC‐LCs. B, The quantitative histogram of flow cytometry assays. C, The measurement of biomark protein expressions of Leydig cells or ADSCs using Western blot in ADSCs, LCs and ADSC‐LCs

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP7639

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Steroidogenic Acute Regulatory protein (StAR) also known as STARD1 plays an important role in steroid hormone synthesis. It facilitates the transport of cholesterol from the outer to the inner mitochondrial membrane essential for steroid biosynthesis. StAR has a molecular mass of approximately 30 kDa. Expression of StAR occurs predominantly in steroidogenic tissues like adrenal glands ovaries and testes. The protein's efficient functioning is necessary for the maintenance of proper steroid hormone levels in the body.

Biological function summary

The transport activity of StAR is important for the production of steroid hormones which are pivotal in numerous physiological functions. StAR does not function alone; it forms a part of a larger complex of proteins involved in mitochondrial cholesterol transfer. By enabling the conversion of cholesterol to pregnenolone a precursor to various steroid hormones StAR directly affects processes such as metabolism immune function and sexual differentiation. Abnormalities in these processes can result from disruptions in StAR's activity.

Pathways

The involvement of StAR in cholesterol transport places it in the important steroidogenic pathway. This pathway includes interactions with other pivotal proteins such as cytochrome P450scc (CYP11A1) which further converts cholesterol into pregnenolone. Another important pathway where StAR participates is the adrenal biosynthesis pathway linking it to the production of glucocorticoids and mineralocorticoids. Disruption in these pathways due to StAR deficiency or malfunction can lead to severe biological implications.

StAR is closely associated with conditions like lipoid congenital adrenal hyperplasia (LCAH) and Addison's disease. Mutations or deficiencies in StAR can interrupt steroid hormone production which is essential for managing these conditions. Additionally proteins like CYP11A1 may show functional impairment in the presence of StAR mutations exacerbating these disorders. A deeper understanding of StAR's role in these conditions can support the development of targeted therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.

See full target information STAR

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Journal of assisted reproduction and genetics 42:923-936 PubMed39810070

2025

miR-361-5p regulates SLC25A24 to maintain mitochondrial function and alleviate granulosa cell dysfunction in diminished ovarian reserve.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyuan Xu,Yan Jia

Cells 11: PubMed36230992

2022

Di-n-Butyl Phthalate and Its Monoester Metabolite Impairs Steroid Hormone Biosynthesis in Human Cells: Mechanistic In Vitro Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Liselott Källsten,Paula Pierozan,Jonathan W Martin,Oskar Karlsson

Molecular and cellular biology 41:e0035721 PubMed34460330

2021

Regulation of Estradiol Synthesis by Aromatase Interacting Partner in Breast (AIPB).

Applications

Unspecified application

Species

Unspecified reactive species

Himangshu S Bose,Randy M Whittal,Curtis E Lanier,Brendan Marshall,Maheshinie Rajapaksha,Brian W Wheeler,Nicholas D Carbo,Elin M Hahn,Elizabeth W Perry,Neal M Hall,Mikhail M Melomed,Edward L Perkins,William E Burak

Cell & bioscience 11:125 PubMed34233737

2021

Single-cell transcriptomic analysis of endometriosis provides insights into fibroblast fates and immune cell heterogeneity.

Applications

Unspecified application

Species

Unspecified reactive species

Junyan Ma,Liqi Zhang,Hong Zhan,Yun Mo,Zuanjie Ren,Anwen Shao,Jun Lin

Journal of cellular and molecular medicine 25:3460-3468 PubMed33713531

2021

H S catalysed by CBS regulates testosterone synthesis through affecting the sulfhydrylation of PDE.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Jing Wang,Tao Shen,Renyun Hong,Shanshan Tang,Xia Zhao

Biology of reproduction 102:145-155 PubMed31504196

2019

Immunodetection and quantification of enzymatic markers in theca cells: the early process of ovarian steroidogenesis†.

Applications

Unspecified application

Species

Unspecified reactive species

P Asiabi,E C R Leonel,E Marbaix,M M Dolmans,C A Amorim

Journal of cellular and molecular medicine 23:5956-5969 PubMed31293077

2019

Differentiation of human adipose derived stem cells into Leydig-like cells with molecular compounds.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Chen,Chao Li,Weiping Ji,Long Wang,Xianwu Chen,Shenzhi Zhao,Zhangye Xu,Renshan Ge,Xiaoling Guo

European journal of medicinal chemistry 176:21-40 PubMed31091478

2019

Synthesis and biological evaluation of methylpyrimidine-fused tricyclic diterpene analogs as novel oral anti-late-onset hypogonadism agents.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Bai,Jia Xie,Yajing Xing,Li-Ting Wang,Jiuqing Xie,Fan Yang,Ting Liu,Mingyao Liu,Jie Tang,Zhengfang Yi,Wen-Wei Qiu

RSC advances 9:9709-9717 PubMed35520707

2019

Discovery of methylpyrimidine ring-fused diterpenoid analogs as a novel testosterone synthesis promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Bai,Jia Xie,Li-Ting Wang,Yajing Xing,Qian-Ru Jiang,Fan Yang,Jie Tang,Zhengfang Yi,Wen-Wei Qiu

Biochemical and biophysical research communications 509:476-482 PubMed30595381

2019

Overexpression of the steroidogenic acute regulatory protein in breast cancer: Regulation by histone deacetylase inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Pulak R Manna,Ahsen U Ahmed,David Vartak,Deborah Molehin,Kevin Pruitt

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-StAR antibody [EP7639]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (AB133657)

Western blot - Anti-StAR antibody [EP7639] (AB133657)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (AB133657)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (AB133657)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (AB133657)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (AB133657)

Western blot - Anti-StAR antibody [EP7639] (AB133657)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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