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AB244236

Anti-StAR antibody [EP7639] - BSA and Azide free

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Rabbit Recombinant Monoclonal STAR antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

STARD1, STAR, StAR, START domain-containing protein 1, StARD1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] - BSA and Azide free (AB244236)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] - BSA and Azide free (AB244236)

This data was developed using ab133657, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labelling StAR with ab133657 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-StAR antibody [EP7639] - BSA and Azide free (AB244236)
  • WB

Unknown

Western blot - Anti-StAR antibody [EP7639] - BSA and Azide free (AB244236)

This data was developed using ab133657, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-StAR antibody [EP7639] (<a href='/en-us/products/primary-antibodies/star-antibody-ep7639-ab133657'>ab133657</a>) at 1/10000 dilution

All lanes:

Human adrenal gland lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 32 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] - BSA and Azide free (AB244236)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] - BSA and Azide free (AB244236)

This data was developed using ab133657, the same antibody clone in a different buffer formulation.

ab133657 showing negative staining in Skeletal muscle tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] - BSA and Azide free (AB244236)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] - BSA and Azide free (AB244236)

This data was developed using ab133657, the same antibody clone in a different buffer formulation.

ab133657 showing positive staining in Normal testis tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] - BSA and Azide free (AB244236)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] - BSA and Azide free (AB244236)

This data was developed using ab133657, the same antibody clone in a different buffer formulation.

ab133657 showing positive staining in Sertoli-Leydig cell tumor tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] - BSA and Azide free (AB244236)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] - BSA and Azide free (AB244236)

This data was developed using ab133657, the same antibody clone in a different buffer formulation.

ab133657 showing positive staining in Adrenocortical carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP7639

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab244236 is the carrier-free version of ab133657.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Steroidogenic Acute Regulatory protein (StAR) also known as STARD1 plays an important role in steroid hormone synthesis. It facilitates the transport of cholesterol from the outer to the inner mitochondrial membrane essential for steroid biosynthesis. StAR has a molecular mass of approximately 30 kDa. Expression of StAR occurs predominantly in steroidogenic tissues like adrenal glands ovaries and testes. The protein's efficient functioning is necessary for the maintenance of proper steroid hormone levels in the body.
Biological function summary

The transport activity of StAR is important for the production of steroid hormones which are pivotal in numerous physiological functions. StAR does not function alone; it forms a part of a larger complex of proteins involved in mitochondrial cholesterol transfer. By enabling the conversion of cholesterol to pregnenolone a precursor to various steroid hormones StAR directly affects processes such as metabolism immune function and sexual differentiation. Abnormalities in these processes can result from disruptions in StAR's activity.

Pathways

The involvement of StAR in cholesterol transport places it in the important steroidogenic pathway. This pathway includes interactions with other pivotal proteins such as cytochrome P450scc (CYP11A1) which further converts cholesterol into pregnenolone. Another important pathway where StAR participates is the adrenal biosynthesis pathway linking it to the production of glucocorticoids and mineralocorticoids. Disruption in these pathways due to StAR deficiency or malfunction can lead to severe biological implications.

StAR is closely associated with conditions like lipoid congenital adrenal hyperplasia (LCAH) and Addison's disease. Mutations or deficiencies in StAR can interrupt steroid hormone production which is essential for managing these conditions. Additionally proteins like CYP11A1 may show functional impairment in the presence of StAR mutations exacerbating these disorders. A deeper understanding of StAR's role in these conditions can support the development of targeted therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
See full target information STAR
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Product promise

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