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AB307801

Anti-StAR antibody [HL1362] - BSA and Azide free

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Rabbit Recombinant Monoclonal STAR antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human STAR.
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Immunocytochemistry/ Immunofluorescence - Anti-StAR antibody [HL1362] - BSA and Azide free (AB307801)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-StAR antibody [HL1362] - BSA and Azide free (AB307801)

Immunofluorescence analysis of 4% paraformaldehyde fixed K562 cells labelling StAR protein at mitochondria using ab307801 at a 1/500 dilution at RT for 15 min (Green). The nuclear counter stain is DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [HL1362] - BSA and Azide free (AB307801)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [HL1362] - BSA and Azide free (AB307801)

Paraffin-embedded mouse kidney tissue stained for StAR protein with ab307801 at a 1/100 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1362

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human STAR. The exact immunogen used to generate this antibody is proprietary information.

P49675

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Reactivity data

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Steroidogenic Acute Regulatory protein (StAR) also known as STARD1 plays an important role in steroid hormone synthesis. It facilitates the transport of cholesterol from the outer to the inner mitochondrial membrane essential for steroid biosynthesis. StAR has a molecular mass of approximately 30 kDa. Expression of StAR occurs predominantly in steroidogenic tissues like adrenal glands ovaries and testes. The protein's efficient functioning is necessary for the maintenance of proper steroid hormone levels in the body.
Biological function summary

The transport activity of StAR is important for the production of steroid hormones which are pivotal in numerous physiological functions. StAR does not function alone; it forms a part of a larger complex of proteins involved in mitochondrial cholesterol transfer. By enabling the conversion of cholesterol to pregnenolone a precursor to various steroid hormones StAR directly affects processes such as metabolism immune function and sexual differentiation. Abnormalities in these processes can result from disruptions in StAR's activity.

Pathways

The involvement of StAR in cholesterol transport places it in the important steroidogenic pathway. This pathway includes interactions with other pivotal proteins such as cytochrome P450scc (CYP11A1) which further converts cholesterol into pregnenolone. Another important pathway where StAR participates is the adrenal biosynthesis pathway linking it to the production of glucocorticoids and mineralocorticoids. Disruption in these pathways due to StAR deficiency or malfunction can lead to severe biological implications.

StAR is closely associated with conditions like lipoid congenital adrenal hyperplasia (LCAH) and Addison's disease. Mutations or deficiencies in StAR can interrupt steroid hormone production which is essential for managing these conditions. Additionally proteins like CYP11A1 may show functional impairment in the presence of StAR mutations exacerbating these disorders. A deeper understanding of StAR's role in these conditions can support the development of targeted therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
See full target information STAR

Alternative Names

STARD1, STAR, StAR, START domain-containing protein 1, StARD1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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