Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (AB251360)

This data was developed using ab202060, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling STRAD4 with ab202060 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on SH-SY5Y cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :
-ve control 1 : ab202060 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

• WB

Supplier Data

Western blot - Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (AB251360)

This data was developed using ab202060, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-STARD4 antibody [EPR17847-32] (<a href='/en-us/products/primary-antibodies/stard4-antibody-epr17847-32-ab202060'>ab202060</a>) at 1/1000 dilution

Lane 1:

Mouse kidney tissue lysate at 10 µg

Lane 2:

Mouse spleen tissue lysate at 10 µg

Lane 3:

Rat kidney tissue lysate at 10 µg

Lane 4:

Rat spleen tissue lysate at 10 µg

Lane 5:

Rat liver tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Exposure time: 3min

• IP

Supplier Data

Immunoprecipitation - Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (AB251360)

This data was developed using ab202060, the same antibody clone in a different buffer formulation.

STARD4 was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate with ab202060 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab202060 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : NIH/3T3 whole cell lysate 10 μg (Input). Lane 2 : ab202060 IP in NIH/3T3 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab202060 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-STARD4 antibody [EPR17847-32] (<a href='/en-us/products/primary-antibodies/stard4-antibody-epr17847-32-ab202060'>ab202060</a>)

Predicted band size: 24 kDa

false

• WB

Supplier Data

Western blot - Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (AB251360)

This data was developed using ab202060, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-STARD4 antibody [EPR17847-32] (<a href='/en-us/products/primary-antibodies/stard4-antibody-epr17847-32-ab202060'>ab202060</a>) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Lane 5:

Mouse liver tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Exposure time: 1min

• WB

Supplier Data

Western blot - Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (AB251360)

This data was developed using ab202060, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-STARD4 antibody [EPR17847-32] (<a href='/en-us/products/primary-antibodies/stard4-antibody-epr17847-32-ab202060'>ab202060</a>) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free (AB251360)

This data was developed using ab202060, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-STARD4 antibody [EPR17847-32] (<a href='/en-us/products/primary-antibodies/stard4-antibody-epr17847-32-ab202060'>ab202060</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 20 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Lane 3:

293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

Lane 4:

U937 (Human histiocytic lymphoma cells) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Exposure time: 3min