AB200344

Anti-STARD4 antibody [EPR17847]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal STARD4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

StAR-related lipid transfer protein 4, START domain-containing protein 4, StARD4, STARD4

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-STARD4 antibody [EPR17847] (AB200344)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STARD4 antibody [EPR17847] (AB200344)

Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling STARD4 with ab200344 at a 1/100 dilution. ab150077 AlexaFluor®488 Goat anti-Rabbit secondary 1/500 dilution. Counterstained using ab7291 anti-Tubulin (mouse mAb) 1/1000 dilution and ab150120 AlexaFluor®594 Goat anti-Mouse secondary 1/500 dilution. Cells were fixed in 100% methanol and permeabilized using 0.1% Triton X-100. Nuclear stain DAPI.

Supplier Data

Western blot - Anti-STARD4 antibody [EPR17847] (AB200344)

All lanes:

Western blot - Anti-STARD4 antibody [EPR17847] (ab200344) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Lane 3:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 24 kDa

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Supplier Data

Western blot - Anti-STARD4 antibody [EPR17847] (AB200344)

All lanes:

Western blot - Anti-STARD4 antibody [EPR17847] (ab200344) at 1/1000 dilution

Lane 1:

SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 20 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Lane 3:

293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

Lane 4:

U-937 (Human histiocytic lymphoma cells) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

false

Carrier free

Anti-STARD4 antibody [EPR17847] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17847

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STARD4 or StAR-related lipid transfer protein 4 is a cytosolic protein involved in cholesterol transport. It is approximately 22 kDa in size. This protein facilitates the shuttling of cholesterol between cellular membranes and the cytoplasm. Expression of STARD4 is found in various tissues but it is notably present in the liver and macrophages. Its role in these tissues supports overall cholesterol homeostasis by regulating intracellular cholesterol distribution.

Biological function summary

This protein participates in intracellular lipid trafficking as part of larger biosynthetic and regulatory processes. It does not function independently but rather interacts with several lipid-binding proteins to control cholesterol availability within the cell. These interactions are essential for maintaining membrane integrity and the synthesis of steroid hormones. The activity of STARD4 directly influences the regulation of lipid metabolism and energy homeostasis.

Pathways

STARD4 contributes to lipid metabolism and homeostasis pathways. It plays a role in the cholesterol transport pathway specifically in concert with proteins like STARD1 and STARD5 which also bind and transfer cholesterol. Its function ensures proper cholesterol distribution required for cellular signaling and membrane structure. The activity of STARD4 intersects with other proteins involved in these pathways such as apolipoproteins and other lipid-binding proteins to maintain lipid balance within tissues particularly in organs like the liver.

STARD4 is associated with conditions linked to cholesterol imbalance such as atherosclerosis and non-alcoholic fatty liver disease (NAFLD). Altered expression or function of STARD4 can disrupt normal cholesterol homeostasis contributing to the development of these disorders. Research indicates that its interactions with proteins such as low-density lipoprotein (LDL) receptors and apolipoprotein E (ApoE) are critical in understanding disease mechanisms. Modification in the function or expression of STARD4 can signify altered lipid transport and contribute to pathogenesis in related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the intracellular transport of cholesterol. Binds cholesterol or other sterols.

See full target information StAR-related lipid transfer protein 4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 42:319 PubMed38008713

2023

Hsa_circ_0009092/miR-665/NLK signaling axis suppresses colorectal cancer progression via recruiting TAMs in the tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Jialin Song,Qing Liu,Lei Han,Tiantian Song,Sihao Huang,Xinyao Zhang,Qiuming He,Chenxi Liang,Shuai Zhu,Bin Xiong

iScience 26:106559 PubMed37123228

2023

Multiomic landscape of immune pathogenesis in Kimura's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xunyao Wu,Anqi Wang,Sumei Zhang,Xiaoxi Wang,Dan Guo,Weiguo Zhu,Yang Jiao,Jiaxin Zhou,Wen Zhang,Linyi Peng,Minghui Duan,Yunyun Fei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-STARD4 antibody [EPR17847]

Immunocytochemistry/ Immunofluorescence - Anti-STARD4 antibody [EPR17847] (AB200344)

Western blot - Anti-STARD4 antibody [EPR17847] (AB200344)

Western blot - Anti-STARD4 antibody [EPR17847] (AB200344)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise