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AB251308

Anti-STARD4 antibody [EPR17847] - BSA and Azide free

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Rabbit Recombinant Monoclonal STARD4 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

View Alternative Names

StAR-related lipid transfer protein 4, START domain-containing protein 4, StARD4, STARD4

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-STARD4 antibody [EPR17847] - BSA and Azide free (AB251308)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STARD4 antibody [EPR17847] - BSA and Azide free (AB251308)

This data was developed using ab200344, the same antibody clone in a different buffer formulation.

Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling STARD4 with ab200344 at a 1/100 dilution. ab150077 AlexaFluor®488 Goat anti-Rabbit secondary, 1/500 dilution. Counterstained using ab7291 anti-Tubulin (mouse mAb), 1/1000 dilution and ab150120 AlexaFluor®594 Goat anti-Mouse secondary, 1/500 dilution. Cells were fixed in 100% methanol and permeabilized using 0.1% Triton X-100. Nuclear stain DAPI.

Western blot - Anti-STARD4 antibody [EPR17847] - BSA and Azide free (AB251308)
  • WB

Supplier Data

Western blot - Anti-STARD4 antibody [EPR17847] - BSA and Azide free (AB251308)

This data was developed using ab200344, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-STARD4 antibody [EPR17847] (<a href='/en-us/products/primary-antibodies/stard4-antibody-epr17847-ab200344'>ab200344</a>) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Lane 3:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 24 kDa

false

Western blot - Anti-STARD4 antibody [EPR17847] - BSA and Azide free (AB251308)
  • WB

Supplier Data

Western blot - Anti-STARD4 antibody [EPR17847] - BSA and Azide free (AB251308)

This data was developed using ab200344, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-STARD4 antibody [EPR17847] (<a href='/en-us/products/primary-antibodies/stard4-antibody-epr17847-ab200344'>ab200344</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 20 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Lane 3:

293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

Lane 4:

U-937 (Human histiocytic lymphoma cells) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17847

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab251308 is the carrier-free version of ab200344.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STARD4 or StAR-related lipid transfer protein 4 is a cytosolic protein involved in cholesterol transport. It is approximately 22 kDa in size. This protein facilitates the shuttling of cholesterol between cellular membranes and the cytoplasm. Expression of STARD4 is found in various tissues but it is notably present in the liver and macrophages. Its role in these tissues supports overall cholesterol homeostasis by regulating intracellular cholesterol distribution.
Biological function summary

This protein participates in intracellular lipid trafficking as part of larger biosynthetic and regulatory processes. It does not function independently but rather interacts with several lipid-binding proteins to control cholesterol availability within the cell. These interactions are essential for maintaining membrane integrity and the synthesis of steroid hormones. The activity of STARD4 directly influences the regulation of lipid metabolism and energy homeostasis.

Pathways

STARD4 contributes to lipid metabolism and homeostasis pathways. It plays a role in the cholesterol transport pathway specifically in concert with proteins like STARD1 and STARD5 which also bind and transfer cholesterol. Its function ensures proper cholesterol distribution required for cellular signaling and membrane structure. The activity of STARD4 intersects with other proteins involved in these pathways such as apolipoproteins and other lipid-binding proteins to maintain lipid balance within tissues particularly in organs like the liver.

STARD4 is associated with conditions linked to cholesterol imbalance such as atherosclerosis and non-alcoholic fatty liver disease (NAFLD). Altered expression or function of STARD4 can disrupt normal cholesterol homeostasis contributing to the development of these disorders. Research indicates that its interactions with proteins such as low-density lipoprotein (LDL) receptors and apolipoprotein E (ApoE) are critical in understanding disease mechanisms. Modification in the function or expression of STARD4 can signify altered lipid transport and contribute to pathogenesis in related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the intracellular transport of cholesterol. Binds cholesterol or other sterols.
See full target information StAR-related lipid transfer protein 4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com