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AB178688

Anti-STARD5 antibody [EPR13545(B)]

5

(1 Review)

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(2 Publications )

Rabbit Recombinant Monoclonal STARD5 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.
4 Images
Flow Cytometry (Intracellular) - Anti-STARD5 antibody [EPR13545(B)] (AB178688)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-STARD5 antibody [EPR13545(B)] (AB178688)

Intracellular flow cytometric analysis of permeabilized 293T cells using ab178688 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

Immunoprecipitation - Anti-STARD5 antibody [EPR13545(B)] (AB178688)
  • IP

Supplier Data

Immunoprecipitation - Anti-STARD5 antibody [EPR13545(B)] (AB178688)

Western blot analysis on immunoprecipitation pellet from (Lane 1) THP-1 cell lysate or (Lane 2) 1X PBS (negative control) using ab178688 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-STARD5 antibody [EPR13545(B)] (ab178688)

Predicted band size: 24 kDa

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Western blot - Anti-STARD5 antibody [EPR13545(B)] (AB178688)
  • WB

Supplier Data

Western blot - Anti-STARD5 antibody [EPR13545(B)] (AB178688)

All lanes:

Western blot - Anti-STARD5 antibody [EPR13545(B)] (ab178688) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

THP-1 lysate at 10 µg

Lane 4:

Human fetal liver tissue lysate at 10 µg

Lane 5:

Human fetal kidney tissue lysate at 10 µg

Lane 6:

HL-60 lysate at 10 µg

Predicted band size: 24 kDa

false

Western blot - Anti-STARD5 antibody [EPR13545(B)] (AB178688)
  • WB

Lab

Western blot - Anti-STARD5 antibody [EPR13545(B)] (AB178688)

All lanes:

Western blot - Anti-STARD5 antibody [EPR13545(B)] (ab178688) at 1/1000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) nuclear lysate at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) cytoplasm lysate at 20 µg

Lane 3:

HEK-293 (Human embryonic kidney epithelial cell) membrane lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 3s

  • Carrier free

    Anti-STARD5 antibody [EPR13545(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13545(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STARD5 also known as StAR-related lipid transfer domain protein 5 is an important player in intracellular cholesterol transport. It functions as a START domain containing protein facilitating the transfer of cholesterol and other lipids between intracellular membranes. With a molecular mass of approximately 23 kDa STARD5 displays a specific pattern of expression in tissues. It is highly expressed in the liver kidney and spleen where it plays a role in cellular lipid regulation.
Biological function summary

The transfer of cholesterol and lipids by STARD5 helps maintain cellular lipid homeostasis. It is not known to form part of a complex but operates independently to ensure that cholesterol levels are balanced within cells. This activity supports cell membrane integrity and the synthesis of steroid hormones and bile acids. By regulating these processes STARD5 plays a significant function in maintaining cell health and metabolism.

Pathways

The activity of STARD5 is critical in the cholesterol transport and metabolism pathway. It works closely with proteins such as NPC1 and NPC2 which also facilitate cholesterol trafficking. This involvement aligns STARD5 with the lysosomal storage pathway highlighting its role in managing cholesterol storage and mobilization within cells.

Altered function or expression of STARD5 links to conditions like Niemann-Pick disease type C and obesity-related dyslipidemia. Niemann-Pick disease type C is closely tied to disrupted cholesterol transport where STARD5's role intersects with NPC1 and NPC2 proteins both known for their involvement in this lysosomal disorder. In obesity-related dyslipidemia the protein's regulation of cholesterol levels can impact lipid profiles influencing disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in the intracellular transport of sterols or other lipids. May bind cholesterol or other sterols (By similarity).
See full target information StAR-related lipid transfer protein 5

Alternative Names

StAR-related lipid transfer protein 5, START domain-containing protein 5, StARD5, STARD5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 23:277 PubMed37978523

2023

Eleven metabolism‑related genes composed of Stard5 predict prognosis and contribute to EMT phenotype in HCC.

Applications

Unspecified application

Species

Unspecified reactive species

Dongping Li,Xiahui Lin,Jiale Li,Xinyi Liu,Feng Zhang,Wenqing Tang,Si Zhang,Ling Dong,Ruyi Xue

Nature structural & molecular biology 29:774-780 PubMed35915257

2022

Mechanism of client selection by the protein quality-control factor UBE2O.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew C J Yip,Samantha F Sedor,Sichen Shao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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