Anti-STARD5 antibody [EPR13545(B)] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-STARD5 antibody [EPR13545(B)] - BSA and Azide free (AB250062)

This data was developed using ab178688, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293T cells using ab178688 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

• IP

Supplier Data

Immunoprecipitation - Anti-STARD5 antibody [EPR13545(B)] - BSA and Azide free (AB250062)

This data was developed using ab178688, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from (Lane 1) THP-1 cell lysate or (Lane 2) 1X PBS (negative control) using ab178688 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-STARD5 antibody [EPR13545(B)] (<a href='/en-us/products/primary-antibodies/stard5-antibody-epr13545b-ab178688'>ab178688</a>)

Predicted band size: 24 kDa

false

• WB

Supplier Data

Western blot - Anti-STARD5 antibody [EPR13545(B)] - BSA and Azide free (AB250062)

This data was developed using ab178688, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-STARD5 antibody [EPR13545(B)] (<a href='/en-us/products/primary-antibodies/stard5-antibody-epr13545b-ab178688'>ab178688</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

THP-1 lysate at 10 µg

Lane 4:

Human fetal liver tissue lysate at 10 µg

Lane 5:

Human fetal kidney tissue lysate at 10 µg

Lane 6:

HL-60 lysate at 10 µg

Predicted band size: 24 kDa

false

• WB

Lab

Western blot - Anti-STARD5 antibody [EPR13545(B)] - BSA and Azide free (AB250062)

All lanes:

Western blot - Anti-STARD5 antibody [EPR13545(B)] (<a href='/en-us/products/primary-antibodies/stard5-antibody-epr13545b-ab178688'>ab178688</a>) at 1/1000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) nuclear lysate at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) cytoplasm lysate at 20 µg

Lane 3:

HEK-293 (Human embryonic kidney epithelial cell) membrane lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 3s