Anti-Stargazin antibody [EPR25387-48] (ab300519)

Rabbit Recombinant Monoclonal Stargazin antibody. Suitable for IP, IHC-Fr, IHC-P, Dot, WB and reacts with Mouse, Human, Rat, Transfected cell line - Mouse samples.

Be the first to review this product! Submit a review

Images

Immunoprecipitation - Anti-Stargazin antibody [EPR25387-48] (AB300519), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	ICC/IF	IHC-Fr	IHC-P	Dot	WB
Human	Tested	Not recommended	Expected	Not recommended	Expected	Tested
Mouse	Tested	Not recommended	Tested	Tested	Expected	Tested
Rat	Expected	Not recommended	Tested	Tested	Expected	Tested
Transfected cell line - Mouse	Not recommended	Not recommended	Not recommended	Not recommended	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line - Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse, Human, Transfected cell line - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/500	Notes -
Species Mouse	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Transfected cell line - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line - Mouse	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat, Mouse, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line - Mouse	Dilution info -	Notes -

Target data

See full target information CACNG2

Function

Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state.

Alternative names

Voltage-dependent calcium channel gamma-2 subunit, Neuronal voltage-gated calcium channel gamma-2 subunit, Transmembrane AMPAR regulatory protein gamma-2, TARP gamma-2, CACNG2

Recommended products

Rabbit Recombinant Monoclonal Stargazin antibody. Suitable for IP, IHC-Fr, IHC-P, Dot, WB and reacts with Mouse, Human, Rat, Transfected cell line - Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR25387-48
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Stargazin also known as γ-2 is a protein that modulates synaptic function by influencing AMPA receptor trafficking and their gating properties in neurons. It is a transmembrane protein with a mass of approximately 38 kDa. Stargazin is highly expressed in the brain predominantly in the cerebellum and hippocampus and is an important member of the transmembrane AMPA receptor regulatory protein (TARP) family. It directly interacts with AMPA receptors to regulate their surface expression and synaptic localization impacting synaptic plasticity and transmission in neurons.

Biological function summary

The protein known as Stargazin serves a pivotal role in the regulation of excitatory synaptic transmission. It forms part of a complex with AMPA receptors facilitating their proper trafficking to the postsynaptic membrane. Stargazin’s interaction with AMPA receptors is not just structural; it also modulates receptor activation and desensitization kinetics impacting neuronal communication. This modulation is essential for synaptic plasticity—a process underlying learning and memory.

Pathways

The interaction of Stargazin with AMPA receptors is central to the glutamatergic signaling pathway. This pathway is important for synaptic strength modulation and long-term potentiation. Stargazin also interacts with other TARP family proteins such as γ-3 γ-4 and γ-8 which further influences AMPA receptor trafficking across various brain regions. This association with different TARP proteins allows it to finely tune synaptic responses and modifies the receptor's contribution to neural signaling.

Associated diseases and disorders

Stargazin plays a significant role in conditions like epilepsy and ataxia. Mutations or dysregulation in Stargazin disrupt AMPA receptor trafficking and synaptic signaling contributing to the abnormal neuronal activity observed in epilepsy. Moreover Stargazin is implicated in type 1 episodic ataxia due to its influence on cerebellar synaptic function which is vital for motor coordination. Stargazin’s interaction with other AMPAR auxiliary subunits can further exacerbate these disorders by altering receptor compositions and neuronal excitability.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunoprecipitation - Anti-Stargazin antibody [EPR25387-48] (ab300519)
Stargazin was immunoprecipitated from 0.35 mg Human cerebellum tissue lysate 10 ug with ab300519 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300519 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Human cerebellum tissue lysate 10 ug Lane 2: ab300519 IP in Human cerebellum tissue lysate Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab300519 in human cerebellum tissue lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 15 seconds
Lane 1: Immunoprecipitation - Anti-Stargazin antibody [EPR25387-48] (ab300519) at 1/30 dilution
Lane 2: Immunoprecipitation - Anti-Stargazin antibody [EPR25387-48] (ab300519) at 1/1000 dilution
All lanes: Human cerebellum tissue lysate at 10 µg
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 15s
Immunoprecipitation - Anti-Stargazin antibody [EPR25387-48] (ab300519)
Stargazin was immunoprecipitated from 0.35 mg Mouse cerebellum tissue lysate 10 ug with ab300519 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300519 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Mouse cerebellum tissue lysate 10 ug Lane 2: ab300519 IP in Mouse cerebellum tissue lysate Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab300519 in mouse cerebellum tissue lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 6 seconds
Lane 1: Immunoprecipitation - Anti-Stargazin antibody [EPR25387-48] (ab300519) at 1/30 dilution
Lane 2: Immunoprecipitation - Anti-Stargazin antibody [EPR25387-48] (ab300519) at 1/1000 dilution
All lanes: Mouse cerebellum tissue lysate at 10 µg
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 6s
Immunohistochemistry (Frozen sections) - Anti-Stargazin antibody [EPR25387-48] (ab300519)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse heart (fresh) tissue labeling Stargazin with ab300519 at 1/500 (1.206 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control (PMID: 11170751)No staining on mouse heart is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution. .
Immunohistochemistry (Frozen sections) - Anti-Stargazin antibody [EPR25387-48] (ab300519)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat heart (fresh) tissue labeling Stargazin with ab300519 at 1/500 (1.206 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control (PMID: 11170751)No staining on rat heart is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution. .
Immunohistochemistry (Frozen sections) - Anti-Stargazin antibody [EPR25387-48] (ab300519)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh) tissue labeling Stargazin with ab300519 at 1/500 (1.206 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebellum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution. .
Immunohistochemistry (Frozen sections) - Anti-Stargazin antibody [EPR25387-48] (ab300519)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling Stargazin with ab300519 at 1/500 (1.206 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution. .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stargazin antibody [EPR25387-48] (ab300519)
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Stargazin with ab300519 at 1/1000 (0.603 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control: no staining in mouse cardiac muscle. The section was incubated with ab300519 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stargazin antibody [EPR25387-48] (ab300519)
Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Stargazin with ab300519 at 1/1000 (0.603 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining in rat cerebral cortex. The section was incubated with ab300519 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stargazin antibody [EPR25387-48] (ab300519)
Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Stargazin with ab300519 at 1/1000 (0.603 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining in mouse hippocampus. The section was incubated with ab300519 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stargazin antibody [EPR25387-48] (ab300519)
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Stargazin with ab300519 at 1/1000 (0.603 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining in mouse cerebellum. The section was incubated with ab300519 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins