Rabbit Polyclonal STAT1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human STAT1 aa 700 to C-terminus.
Images
Publications
Filter by
- EBioMedicine 70:1034772021Expression characteristics of interferon-stimulated genes and possible regulatory mechanisms in lupus patients using transcriptomics analyses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiyao Deng,Ying Zheng,Delun Li,Quan Hong,Min Zhang,Qinggang Li,Bo Fu,Lingling Wu,Xu Wang,Wanjun Shen,Yingjie Zhang,Jiakai Chang,Kangkang Song,Xiaomin Liu,Shunlai Shang,Guangyan Cai,Xiangmei ChenPubMed 34284174
- International journal of molecular medicine 46:239-2512020Puerarin improves graft bone defect through microRNA‑155‑3p‑mediated p53/TNF‑α/STAT1 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Zhou et. al.PubMed 32377717
- Molecular medicine reports 20:4993-50012019IL‑1β increases the expression of inflammatory factors in synovial fluid‑derived fibroblast‑like synoviocytes via activation of the NF‑κB‑mediated ERK‑STAT1 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Yang et. al.PubMed 31638264
- The Journal of neuroscience : the official journal of the Society for Neuroscience 38:3890-39002018Major Vault Protein, a Candidate Gene in 16p11.2 Microdeletion Syndrome, Is Required for the Homeostatic Regulation of Visual Cortical Plasticity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJacque P K Ip,Ikue Nagakura,Jeremy Petravicz,Keji Li,Erik A C Wiemer,Mriganka SurPubMed 29540554
- Cell death discovery 4:42018Inhibition of the MID1 protein complex: a novel approach targeting APP protein synthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrank Matthes et. al.PubMed 29531801
- The Journal of neuroscience : the official journal of the Society for Neuroscience 34:10256-632014STAT1 regulates the homeostatic component of visual cortical plasticity via an AMPA receptor-mediated mechanism.Applications:Unspecified applicationReactive species:MouseIkue Nagakura,Audra Van Wart,Jeremy Petravicz,Daniela Tropea,Mriganka SurPubMed 25080587
- The Journal of general virology 90:2147-562009Parainfluenza virus 5 genomes are located in viral cytoplasmic bodies whilst the virus dismantles the interferon-induced antiviral state of cells.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human STAT1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P42224
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
Species Rat | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors (PubMed:12764129, PubMed:12855578, PubMed:15322115, PubMed:23940278, PubMed:34508746, PubMed:35568036, PubMed:9724754). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426, PubMed:35568036). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state (PubMed:28753426, PubMed:35568036). In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state (PubMed:8156998). Becomes activated in response to KITLG/SCF and KIT signaling (PubMed:15526160). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:19088846). Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylated at Thr-749 by IKBKB which promotes binding of STAT1 to the 5'-TTTGAGGC-3' sequence in the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 (PubMed:32209697). Phosphorylation at Thr-749 also promotes binding of STAT1 to the 5'-TTTGAGTC-3' sequence in the IL12B promoter and activation of IL12B transcription (PubMed:32209697). Involved in food tolerance in small intestine: associates with the Gasdermin-D, p13 cleavage product (13 kDa GSDMD) and promotes transcription of CIITA, inducing type 1 regulatory T (Tr1) cells in upper small intestine (By similarity).
Alternative names
Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1
Recommended products
Rabbit Polyclonal STAT1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human STAT1 aa 700 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human STAT1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P42224
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
STAT1 alpha also known as Signal Transducer and Activator of Transcription 1-alpha is a protein with a mass of approximately 91 kDa. It operates in the cell nucleus and cytoplasm to mediate signal transduction and activation of transcription. STAT1 alpha does this by dimerizing following phosphorylation enabling its nuclear translocation. It is broadly expressed in many tissues including immune cells. This expression plays an important role in response to cytokines and other signaling molecules.
- Biological function summary
STAT1 alpha functions as a critical component of the interferon signaling pathway. It is part of a transcription factor complex that regulates the expression of genes involved in cell proliferation differentiation and immune response. STAT1 alpha mediates its effects by forming homo- or heterodimers with related proteins and binding to specific DNA sequences. This interaction facilitates the transcription of genes necessary for innate and adaptive immunity highlighting its fundamental role in host defense mechanisms.
- Pathways
STAT1 alpha participates in the JAK-STAT signaling pathway and interferon signaling pathways. It interacts with Janus kinases (JAKs) after cytokine stimulation resulting in its phosphorylation and dimerization. This pathway links it with other STAT family members such as STAT2 and STAT3 to regulate gene expression. These pathways ensure proper immune function and cell growth control indicating STAT1 alpha's essential role in various cellular processes.
- Associated diseases and disorders
STAT1 alpha is associated with autoimmune diseases and certain cancers. Its malfunction can result in inadequate immune responses or excessive inflammation. In some cancers altered STAT1 alpha activity contributes to unchecked cell proliferation. STAT1 alpha interacts with various proteins such as IFNGR1 and IRF1 in these disease states affecting their signaling pathways. Its dysregulation indicates a potential target for therapeutic intervention in these disorders making it of interest for medical research.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 alpha antibody (ab2071)
Immunofluorescence of STAT1 in human colon tissue with ab2071 at 20 μg/mL.
Western blot - Anti-STAT1 alpha antibody (ab2071)
All lanes: Western blot - Anti-STAT1 alpha antibody (ab2071) at 0.5 µg/mL
Lane 1: Mouse skin at 15 µg
Lane 2: Mouse skeletal muscle at 15 µg
Lane 3: Mouse bladder at 15 µg
Lane 4: Mouse lung at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 87 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 alpha antibody (ab2071)
Immunohistochemistry of STAT1 in human colon tissue with ab2071 at 2.5 μg/ml.
Western blot - Anti-STAT1 alpha antibody (ab2071)
All lanes: Western blot - Anti-STAT1 alpha antibody (ab2071) at 1 µg/mL
All lanes: YB2/0 at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 87 kDa
Western blot - Anti-STAT1 alpha antibody (ab2071)
All lanes: Western blot - Anti-STAT1 alpha antibody (ab2071) at 1 µg/mL
Lane 1: HeLa whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: A431 whole cell lysate
Lane 4: K562 whole cell lysate
Lane 5: NIH3T3 whole cell lysate
Predicted band size: 87 kDa
Observed band size: 91 kDa
Immunocytochemistry/ Immunofluorescence - Anti-STAT1 alpha antibody (ab2071)
Immunocytochemistry of STAT1a in HeLa cells with STAT1a antibody at 10 µg/ml.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com