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Rabbit Recombinant Monoclonal STAT1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 21 publications.


Images

Immunoprecipitation - Anti-STAT1 alpha antibody [EPYR2154] (AB92506), expandable thumbnail
  • Western blot - Anti-STAT1 alpha antibody [EPYR2154] (AB92506), expandable thumbnail
  • Western blot - Anti-STAT1 alpha antibody [EPYR2154] (AB92506), expandable thumbnail
  • Western blot - Anti-STAT1 alpha antibody [EPYR2154] (AB92506), expandable thumbnail
  • Western blot - Anti-STAT1 alpha antibody [EPYR2154] (AB92506), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWB
Human
Tested
Not recommended
Tested
Mouse
Expected
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/20 - 1/50
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Human
Dilution info
-
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/2000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/2000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors (PubMed:12764129, PubMed:12855578, PubMed:15322115, PubMed:23940278, PubMed:34508746, PubMed:35568036, PubMed:9724754). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426, PubMed:35568036). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state (PubMed:28753426, PubMed:35568036). In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state (PubMed:8156998). Becomes activated in response to KITLG/SCF and KIT signaling (PubMed:15526160). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:19088846). Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylated at Thr-749 by IKBKB which promotes binding of STAT1 to the 5'-TTTGAGGC-3' sequence in the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 (PubMed:32209697). Phosphorylation at Thr-749 also promotes binding of STAT1 to the 5'-TTTGAGTC-3' sequence in the IL12B promoter and activation of IL12B transcription (PubMed:32209697). Involved in food tolerance in small intestine: associates with the Gasdermin-D, p13 cleavage product (13 kDa GSDMD) and promotes transcription of CIITA, inducing type 1 regulatory T (Tr1) cells in upper small intestine (By similarity).

Alternative names

Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1

Recommended products

Rabbit Recombinant Monoclonal STAT1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 21 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPYR2154
Purification technique
Affinity purification Protein A
Specificity

Based on Blast results using the immunogen sequence, this antibody should recognise the alpha but not the beta form (only 30% homology) of Stat1.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

STAT1 alpha also known as Signal Transducer and Activator of Transcription 1-alpha is a protein with a mass of approximately 91 kDa. It operates in the cell nucleus and cytoplasm to mediate signal transduction and activation of transcription. STAT1 alpha does this by dimerizing following phosphorylation enabling its nuclear translocation. It is broadly expressed in many tissues including immune cells. This expression plays an important role in response to cytokines and other signaling molecules.

Biological function summary

STAT1 alpha functions as a critical component of the interferon signaling pathway. It is part of a transcription factor complex that regulates the expression of genes involved in cell proliferation differentiation and immune response. STAT1 alpha mediates its effects by forming homo- or heterodimers with related proteins and binding to specific DNA sequences. This interaction facilitates the transcription of genes necessary for innate and adaptive immunity highlighting its fundamental role in host defense mechanisms.

Pathways

STAT1 alpha participates in the JAK-STAT signaling pathway and interferon signaling pathways. It interacts with Janus kinases (JAKs) after cytokine stimulation resulting in its phosphorylation and dimerization. This pathway links it with other STAT family members such as STAT2 and STAT3 to regulate gene expression. These pathways ensure proper immune function and cell growth control indicating STAT1 alpha's essential role in various cellular processes.

Associated diseases and disorders

STAT1 alpha is associated with autoimmune diseases and certain cancers. Its malfunction can result in inadequate immune responses or excessive inflammation. In some cancers altered STAT1 alpha activity contributes to unchecked cell proliferation. STAT1 alpha interacts with various proteins such as IFNGR1 and IRF1 in these disease states affecting their signaling pathways. Its dysregulation indicates a potential target for therapeutic intervention in these disorders making it of interest for medical research.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunoprecipitation - Anti-STAT1 alpha antibody [EPYR2154] (ab92506), expandable thumbnail

    Immunoprecipitation - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

    ab92506 (purified) at 1/20 dilution (0.5ug) immunoprecipitating STAT1 alpha in A431 whole cell lysate. A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab92506 & A431 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab92506 in A431 whole cell lysate
    For western blotting, VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/2000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

    Predicted band size: 87 kDa

  • Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506), expandable thumbnail

    Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

    Lanes 1 - 2: Merged signal (red and green). Green - ab92506 observed at 85 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

    ab92506 was shown to react with STAT1 alpha in wild-type HeLa cells in Western blot with loss of signal observed in STAT1 knockout cell line Human STAT1 knockout HeLa cell line ab255346 (STAT1 knockout cell lysate Human STAT1 knockout HeLa cell lysate ab263837). Wild-type HeLa and STAT1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab92506 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: STAT1 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human STAT1 knockout HeLa cell line (Human STAT1 knockout HeLa cell line ab255346)

    Performed under reducing conditions.

    Predicted band size: 87 kDa

    Observed band size: 85 kDa

  • Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506), expandable thumbnail

    Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: STAT1 alpha knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)
    Lane 4: A431 whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab92506 (unpurified) observed at 90 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab92506 was shown to specifically react with STAT1 alpha when STAT1 alpha knockout samples were used. Wild-type and STAT1 alpha knockout samples were subjected to SDS-PAGE. ab92506 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

    Predicted band size: 87 kDa

  • Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506), expandable thumbnail

    Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

    All lanes: Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506) at 1/1000 dilution

    Lane 1: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Mouse brain lysate at 20 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 87 kDa

    Observed band size: 91 kDa

  • Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506), expandable thumbnail

    Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

    Phospho STAT1 protein is revealed by Anti-STAT1 (phospho S727) antibody [EPR3146] ab109461, anti-STAT1 (phospho S727) antibody. ab92506 is used as pan control which detects total STAT1.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506) at 1/2000 dilution

    Lane 1: Untreated HeLa (human cervix adenocarcinoma) membrane at 10 µg

    Lane 2: HeLa (human cervix adenocarcinoma) membrane treated with Alkaline Phosphatase at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1500 dilution

    Developed using the ECL technique.

    Predicted band size: 87 kDa

    Observed band size: 91 kDa

    Exposure time: 3min

  • Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506), expandable thumbnail

    Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

    All lanes: Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: 293 cell lysate at 10 µg

    Lane 3: NIH-3T3 cell lysate at 10 µg

    Lane 4: A431 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 87 kDa

    Observed band size: 91 kDa

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