AB281999

Anti-STAT1 antibody [1/Stat1]

BOND RX™ Validated
Recombinant
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(3 Publications)

Mouse Recombinant Monoclonal STAT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications.

View Alternative Names

Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [1/Stat1] (AB281999)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [1/Stat1] (AB281999)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling STAT1 with ab281999 at 1/2000 (0.511 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in human tonsil. The section was incubated with ab281999 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [1/Stat1] (AB281999)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling STAT1 with ab281999 at 1/2000 (0.511 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in mouse spleen.The section was incubated with ab281999 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [1/Stat1] (AB281999)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [1/Stat1] (AB281999)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized treated and untreated RAW 264.7 cells labelling STAT1 with ab281999 at 1/25 (40.88 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in RAW 264.7 cells treated with IFN-y (10 ng/ml) for 30 min. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Lab

Western blot - Anti-STAT1 antibody [1/Stat1] (AB281999)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : Lanes 1-6 : 37 seconds;

Lanes 7-9 : 3 minutes.

All lanes:

Western blot - Anti-STAT1 antibody [1/Stat1] (ab281999) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

A549 (human lung carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 4:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 5:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

Lane 6:

Human colon cancer lysate at 10 µg

Lane 7:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

Lane 8:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

Lane 9:

C2C12 (mouse myoblasts myoblast), whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 87 kDa

Observed band size: 87 kDa

false

Supplier Data

Western blot - Anti-STAT1 antibody [1/Stat1] (AB281999)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure times : Lanes 1-2 : 37 seconds ;

Lanes 3-4 : 26 seconds

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 19478064, 26859759).

All lanes:

Western blot - Anti-STAT1 antibody [1/Stat1] (ab281999) at 1/1000 dilution

Lane 1:

Untreated HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HeLa treated with 10 ng/ml IFN-gamma for 30 minutes, whole cell lysate at 10 µg

Lane 3:

Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

Lane 4:

RAW264.7 treated with 50 ng/ml IFN-gamma for 24 hours, whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 87 kDa

Observed band size: 87 kDa

false

Carrier free

Anti-STAT1 antibody [1/Stat1] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1/Stat1

Isotype

IgG1

Carrier free

Reacts with

Human, Mouse

Applications

ICC/IF, IHC-P, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/25", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/25", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Signal Transducer and Activator of Transcription 1 (STAT1) is a protein of about 91 kDa commonly known simply as STAT1. It belongs to the STAT protein family which is involved in gene regulation. STAT1 expresses widely in many tissues including the immune system where it plays a major role. As an important player in cellular responses to cytokines and growth factors STAT1 becomes activated through phosphorylation often referred to as phospho-STAT1. This phosphorylation promotes dimerization an important step for its function.

Biological function summary

The STAT1 protein influences cell survival and immune responses. It functions as a transcription factor and participates in the interferon signaling pathway. In this setting it forms a complex with other STAT proteins such as STAT2 and STAT3 to regulate gene expression. By binding to specific DNA sequences in the nucleus STAT1 alters transcription in response to extracellular signals. It contributes to antiviral defense mechanisms and antiproliferative activities in various cells.

Pathways

The STAT1 protein plays essential roles in the JAK-STAT signaling pathway and interferon signaling pathway. It operates closely with proteins such as JAK1 and TYK2 which are involved in the phosphorylation process activating STAT1. During this activation STAT1 helps initiate cellular responses to interferons a type of signaling protein released during viral infections. The JAK-STAT pathway facilitates various physiological processes including immune responses and cell growth regulation.

STAT1 dysregulation often connects to autoimmune diseases and infectious diseases. Elevated STAT1 activity associates with disorders like systemic lupus erythematosus where an imbalanced immune response occurs. Phosphorylation of STAT1 can also relate to chronic infections in cases where proper immune response activation becomes impaired. In certain cancers STAT1 interaction with proteins like SOCS1 might contribute to tumor immune evasion highlighting its vital role in pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors (PubMed : 12764129, PubMed : 12855578, PubMed : 15322115, PubMed : 23940278, PubMed : 34508746, PubMed : 35568036, PubMed : 9724754). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed : 28753426, PubMed : 35568036). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state (PubMed : 28753426, PubMed : 35568036). In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed : 26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state (PubMed : 8156998). Becomes activated in response to KITLG/SCF and KIT signaling (PubMed : 15526160). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 19088846). Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylated at Thr-749 by IKBKB which promotes binding of STAT1 to the 5'-TTTGAGGC-3' sequence in the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 (PubMed : 32209697). Phosphorylation at Thr-749 also promotes binding of STAT1 to the 5'-TTTGAGTC-3' sequence in the IL12B promoter and activation of IL12B transcription (PubMed : 32209697). Involved in food tolerance in small intestine : associates with the Gasdermin-D, p13 cleavage product (13 kDa GSDMD) and promotes transcription of CIITA, inducing type 1 regulatory T (Tr1) cells in upper small intestine (By similarity).

See full target information STAT1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1472425 PubMed39660129

2024

Repression of JAK2-STAT1 and PD-L1 by CEP-33779 ameliorates the LPS-induced decline in phagocytic activity of alveolar macrophages and mitigates lung injury in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Han Wang,A-Guo Li,Hong-Yan Wang,Yong-Sheng Tu

Cell reports 42:113395 PubMed37967557

2023

Single-cell transcriptomics of human traumatic brain injury reveals activation of endogenous retroviruses in oligodendroglia.

Applications

Unspecified application

Species

Unspecified reactive species

Raquel Garza,Yogita Sharma,Diahann A M Atacho,Arun Thiruvalluvan,Sami Abu Hamdeh,Marie E Jönsson,Vivien Horvath,Anita Adami,Martin Ingelsson,Patric Jern,Molly Gale Hammell,Elisabet Englund,Agnete Kirkeby,Johan Jakobsson,Niklas Marklund

eLife 12: PubMed37092726

2023

Human DUX4 and mouse Dux interact with STAT1 and broadly inhibit interferon-stimulated gene induction.

Applications

Unspecified application

Species

Unspecified reactive species

Amy E Spens,Nicholas A Sutliff,Sean R Bennett,Amy E Campbell,Stephen J Tapscott

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-STAT1 antibody [1/Stat1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [1/Stat1] (AB281999)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [1/Stat1] (AB281999)

Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [1/Stat1] (AB281999)

Western blot - Anti-STAT1 antibody [1/Stat1] (AB281999)

Western blot - Anti-STAT1 antibody [1/Stat1] (AB281999)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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