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Mouse Recombinant Monoclonal STAT1 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples.

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Images

Western blot - Anti-STAT1 antibody [1/Stat1] - BSA and Azide free (AB282026), expandable thumbnail
  • Western blot - Anti-STAT1 antibody [1/Stat1] - BSA and Azide free (AB282026), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [1/Stat1] - BSA and Azide free (AB282026), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [1/Stat1] - BSA and Azide free (AB282026), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [1/Stat1] - BSA and Azide free (AB282026), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IF
Human
Not recommended
Tested
Tested
Not recommended
Mouse
Not recommended
Tested
Tested
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Target data

Function

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors (PubMed:12764129, PubMed:12855578, PubMed:15322115, PubMed:23940278, PubMed:34508746, PubMed:35568036, PubMed:9724754). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426, PubMed:35568036). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state (PubMed:28753426, PubMed:35568036). In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state (PubMed:8156998). Becomes activated in response to KITLG/SCF and KIT signaling (PubMed:15526160). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:19088846). Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylated at Thr-749 by IKBKB which promotes binding of STAT1 to the 5'-TTTGAGGC-3' sequence in the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 (PubMed:32209697). Phosphorylation at Thr-749 also promotes binding of STAT1 to the 5'-TTTGAGTC-3' sequence in the IL12B promoter and activation of IL12B transcription (PubMed:32209697). Involved in food tolerance in small intestine: associates with the Gasdermin-D, p13 cleavage product (13 kDa GSDMD) and promotes transcription of CIITA, inducing type 1 regulatory T (Tr1) cells in upper small intestine (By similarity).

Alternative names

Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1

Recommended products

Mouse Recombinant Monoclonal STAT1 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Carrier free
Yes
Clone number
1/Stat1
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab282026 is the carrier-free version of Anti-STAT1 antibody [1/Stat1] ab281999.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Signal Transducer and Activator of Transcription 1 (STAT1) is a protein of about 91 kDa commonly known simply as STAT1. It belongs to the STAT protein family which is involved in gene regulation. STAT1 expresses widely in many tissues including the immune system where it plays a major role. As an important player in cellular responses to cytokines and growth factors STAT1 becomes activated through phosphorylation often referred to as phospho-STAT1. This phosphorylation promotes dimerization an important step for its function.

Biological function summary

The STAT1 protein influences cell survival and immune responses. It functions as a transcription factor and participates in the interferon signaling pathway. In this setting it forms a complex with other STAT proteins such as STAT2 and STAT3 to regulate gene expression. By binding to specific DNA sequences in the nucleus STAT1 alters transcription in response to extracellular signals. It contributes to antiviral defense mechanisms and antiproliferative activities in various cells.

Pathways

The STAT1 protein plays essential roles in the JAK-STAT signaling pathway and interferon signaling pathway. It operates closely with proteins such as JAK1 and TYK2 which are involved in the phosphorylation process activating STAT1. During this activation STAT1 helps initiate cellular responses to interferons a type of signaling protein released during viral infections. The JAK-STAT pathway facilitates various physiological processes including immune responses and cell growth regulation.

Associated diseases and disorders

STAT1 dysregulation often connects to autoimmune diseases and infectious diseases. Elevated STAT1 activity associates with disorders like systemic lupus erythematosus where an imbalanced immune response occurs. Phosphorylation of STAT1 can also relate to chronic infections in cases where proper immune response activation becomes impaired. In certain cancers STAT1 interaction with proteins like SOCS1 might contribute to tumor immune evasion highlighting its vital role in pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-STAT1 antibody [1/Stat1] - BSA and Azide free (ab282026), expandable thumbnail

    Western blot - Anti-STAT1 antibody [1/Stat1] - BSA and Azide free (ab282026)

    This data was developed using Anti-STAT1 antibody [1/Stat1] ab281999, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: Lanes 1-6: 37 seconds ;

    Lanes 7-9: 3 minutes.

    All lanes: Western blot - Anti-STAT1 antibody [1/Stat1] (Anti-STAT1 antibody [1/Stat1] ab281999) at 1/1000 dilution

    Lane 1: A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 2: A549 (human lung carcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 3: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 4: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 5: HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

    Lane 6: Human colon cancer lysate at 10 µg

    Lane 7: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

    Lane 8: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

    Lane 9: C2C12 (mouse myoblasts myoblast), whole cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 87 kDa

    Observed band size: 87 kDa

  • Western blot - Anti-STAT1 antibody [1/Stat1] - BSA and Azide free (ab282026), expandable thumbnail

    Western blot - Anti-STAT1 antibody [1/Stat1] - BSA and Azide free (ab282026)

    This data was developed using Anti-STAT1 antibody [1/Stat1] ab281999 the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure times: Lanes 1-2: 37 seconds ;

    Lanes 3-4: 26 seconds.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 19478064, 26859759).

    All lanes: Western blot - Anti-STAT1 antibody [1/Stat1] - BSA and Azide free (ab282026) at 1/1000 dilution

    Lane 1: Untreated HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 2: HeLa treated with 10 ng/ml IFN-gamma for 30 minutes, whole cell lysate at 10 µg

    Lane 3: Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

    Lane 4: RAW264.7 treated with 50 ng/ml IFN-gamma for 24 hours, whole cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 87 kDa

    Observed band size: 87 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [1/Stat1] - BSA and Azide free (ab282026), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [1/Stat1] - BSA and Azide free (ab282026)

    This data was developed using Anti-STAT1 antibody [1/Stat1] ab281999 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling STAT1 with Anti-STAT1 antibody [1/Stat1] ab281999 at 1/2000 (0.511 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in human tonsil. The section was incubated with Anti-STAT1 antibody [1/Stat1] ab281999 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [1/Stat1] - BSA and Azide free (ab282026), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [1/Stat1] - BSA and Azide free (ab282026)

    This data was developed using Anti-STAT1 antibody [1/Stat1] ab281999 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling STAT1 with Anti-STAT1 antibody [1/Stat1] ab281999 at 1/2000 (0.511 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in mouse spleen.The section was incubated with Anti-STAT1 antibody [1/Stat1] ab281999 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [1/Stat1] - BSA and Azide free (ab282026), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [1/Stat1] - BSA and Azide free (ab282026)

    This data was developed using Anti-STAT1 antibody [1/Stat1] ab281999 the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized treated and untreated RAW 264.7 cells labelling STAT1 with Anti-STAT1 antibody [1/Stat1] ab281999 at 1/25 (40.88 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in RAW 264.7 cells treated with IFN-y (10 ng/ml) for 30 min. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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