AB155933

Anti-STAT1 antibody [15H3]

Name: Anti-STAT1 antibody [15H3] (ab155933) | Abcam
Brand: Abcam Limited
SKU: AB155933

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(7 Publications)

Mouse Monoclonal STAT1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, African green monkey, Primates samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human STAT1.

View Alternative Names

Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [15H3] (AB155933)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [15H3] (AB155933)

Immunofluorescent analysis of formaldehyde-fixed HeLa cells labeling STAT1 with ab155933 at 1/20 dilution (green, left image compared with control cells (right image) followed by a DyLight 488-conjugated secondary. Actin stained with Phalloidin (red) and nuclei stained with DAPI (blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [15H3] (AB155933)

Immunofluorescent analysis of formaldehyde-fixed A2058 cells labeling STAT1 with ab155933 at 1/20 dilution (green, left image compared with control cells (right image) followed by a DyLight 488-conjugated secondary. Actin stained with Phalloidin (red) and nuclei stained with DAPI (blue).

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [15H3] (AB155933)

Immunofluorescent analysis of formaldehyde-fixed MCF7 cells labeling STAT1 with ab155933 at 1/20 dilution (green, left image compared with control cells (right image) followed by a DyLight 488-conjugated secondary. Actin stained with Phalloidin (red) and nuclei stained with DAPI (blue).

Unknown

Immunoprecipitation - Anti-STAT1 antibody [15H3] (AB155933)

Immunoprecipitation analysis of STAT1 in U2OS cells.
750 µg of U2OS cell lysate immunoprecipitated with 2 µg ab155933.
All lanes : ab155933 at 1/1000 dilution.
Lane 1 : U2OS whole cell lysate.
Lane 2 : U2OS whole cell lysate immunopreciptated with ab155933.
Western Blot : 4-20% Tris-HCl polyacrylamide gel.
Secondary : Goat anti-mouse HRP conjugated antibody at 1/20000 dilution.
Predicted band size : 87 kDa.

All lanes:

Immunoprecipitation - Anti-STAT1 antibody [15H3] (ab155933)

Predicted band size: 87 kDa

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Unknown

Western blot - Anti-STAT1 antibody [15H3] (AB155933)

SDS PAGE : 4-20% Tris-HCl polyacrylamide gel.

All lanes:

Western blot - Anti-STAT1 antibody [15H3] (ab155933) at 1/1000 dilution

Lane 1:

A549 whole cell lysate at 25 µg

Lane 2:

293T whole cell lysate at 25 µg

Lane 3:

Jurkat whole cell lysate at 25 µg

Lane 4:

A431 whole cell lysate at 25 µg

Lane 5:

U2OS whole cell lysate at 25 µg

Lane 6:

COS7 whole cell lysate at 25 µg

Lane 7:

3T3L1 whole cell lysate at 25 µg

Secondary

All lanes:

Goat anti-mouse HRP conjugated antibody at 1/20000 dilution

Predicted band size: 87 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

15H3

Isotype

IgG1

Carrier free

Reacts with

Human, Primates, African green monkey

Applications

IP, WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human STAT1.

P42224

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20", "ICCIF-species-notes": "" }, "African green monkey": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Primates": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "2 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/20", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Signal Transducer and Activator of Transcription 1 (STAT1) is a protein of about 91 kDa commonly known simply as STAT1. It belongs to the STAT protein family which is involved in gene regulation. STAT1 expresses widely in many tissues including the immune system where it plays a major role. As an important player in cellular responses to cytokines and growth factors STAT1 becomes activated through phosphorylation often referred to as phospho-STAT1. This phosphorylation promotes dimerization an important step for its function.

Biological function summary

The STAT1 protein influences cell survival and immune responses. It functions as a transcription factor and participates in the interferon signaling pathway. In this setting it forms a complex with other STAT proteins such as STAT2 and STAT3 to regulate gene expression. By binding to specific DNA sequences in the nucleus STAT1 alters transcription in response to extracellular signals. It contributes to antiviral defense mechanisms and antiproliferative activities in various cells.

Pathways

The STAT1 protein plays essential roles in the JAK-STAT signaling pathway and interferon signaling pathway. It operates closely with proteins such as JAK1 and TYK2 which are involved in the phosphorylation process activating STAT1. During this activation STAT1 helps initiate cellular responses to interferons a type of signaling protein released during viral infections. The JAK-STAT pathway facilitates various physiological processes including immune responses and cell growth regulation.

STAT1 dysregulation often connects to autoimmune diseases and infectious diseases. Elevated STAT1 activity associates with disorders like systemic lupus erythematosus where an imbalanced immune response occurs. Phosphorylation of STAT1 can also relate to chronic infections in cases where proper immune response activation becomes impaired. In certain cancers STAT1 interaction with proteins like SOCS1 might contribute to tumor immune evasion highlighting its vital role in pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors (PubMed : 12764129, PubMed : 12855578, PubMed : 15322115, PubMed : 23940278, PubMed : 34508746, PubMed : 35568036, PubMed : 9724754). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed : 28753426, PubMed : 35568036). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state (PubMed : 28753426, PubMed : 35568036). In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed : 26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state (PubMed : 8156998). Becomes activated in response to KITLG/SCF and KIT signaling (PubMed : 15526160). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 19088846). Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylated at Thr-749 by IKBKB which promotes binding of STAT1 to the 5'-TTTGAGGC-3' sequence in the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 (PubMed : 32209697). Phosphorylation at Thr-749 also promotes binding of STAT1 to the 5'-TTTGAGTC-3' sequence in the IL12B promoter and activation of IL12B transcription (PubMed : 32209697). Involved in food tolerance in small intestine : associates with the Gasdermin-D, p13 cleavage product (13 kDa GSDMD) and promotes transcription of CIITA, inducing type 1 regulatory T (Tr1) cells in upper small intestine (By similarity).

See full target information STAT1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:3007 PubMed37230975

2023

HDAC9-mediated epithelial cell cycle arrest in G2/M contributes to kidney fibrosis in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhang,Yujie Yang,Fan Yang,Xiaohan Liu,Ping Zhan,Jichao Wu,Xiaojie Wang,Ziying Wang,Wei Tang,Yu Sun,Yan Zhang,Qianqian Xu,Jin Shang,Junhui Zhen,Min Liu,Fan Yi

Cell reports 41:111561 PubMed36288705

2022

OTUB2 exerts tumor-suppressive roles via STAT1-mediated CALML3 activation and increased phosphatidylserine synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Wan Chang,Qingyu Luo,Xiaowei Wu,Yabing Nan,Pengfei Zhao,Lingqiang Zhang,Aiping Luo,Wenjie Jiao,Qiong Zhu,Yesheng Fu,Zhihua Liu

Diagnostic pathology 16:93 PubMed34689819

2021

CircRNA NRIP1 promotes papillary thyroid carcinoma progression by sponging mir-195-5p and modulating the P38 MAPK and JAK/STAT pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Li,Lijuan Zhu,Lijun Fu,Mingli Han,Ya Li,Zhaozhong Meng,Xinguang Qiu

PLoS pathogens 17:e1009860 PubMed34432853

2021

Robust innate immune responses at the placenta during early gestation may limit in utero HIV transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Erica L Johnson,Dominika Swieboda,Amanda Olivier,Elizabeth Ann L Enninga,Rana Chakraborty

Frontiers in aging neuroscience 13:671206 PubMed34113246

2021

Cornel Iridoid Glycoside Protects Against STAT1-Dependent Synapse and Memory Deficits by Increasing -Methyl-D-aspartate Receptor Expression in a Tau Transgenic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Denglei Ma,Rui Huang,Kaiwen Guo,Zirun Zhao,Weipeng Wei,Lihong Gu,Lin Li,Lan Zhang

The Journal of experimental medicine 217: PubMed32181805

2020

Glioma stem-like cells evade interferon suppression through MBD3/NuRD complex-mediated STAT1 downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Zhan,Saisai Guo,Yuanyuan Li,Haowen Ran,Haohao Huang,Lanjuan Mi,Jin Wu,Xinzheng Wang,Dake Xiao,Lishu Chen,Da Li,Songyang Zhang,Xu Yan,Yu Yu,Tingting Li,Qiuying Han,Kun He,Jiuwei Cui,Tao Li,Tao Zhou,Jeremy N Rich,Shideng Bao,Xuemin Zhang,Ailing Li,Jianghong Man

Oncology reports 35:3630-8 PubMed27109360

2016

NF-YA promotes invasion and angiogenesis by upregulating EZH2-STAT3 signaling in human melanoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zihan Xu,Yaowen Sun,Yadong Guo,Gaoping Qin,Shengzhi Mu,Ronghui Fan,Benfeng Wang,Wenjie Gao,Hangli Wu,Guodong Wang,Zhenxin Zhang

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-STAT1 antibody [15H3]

Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [15H3] (AB155933)

Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [15H3] (AB155933)

Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [15H3] (AB155933)

Immunoprecipitation - Anti-STAT1 antibody [15H3] (AB155933)

Western blot - Anti-STAT1 antibody [15H3] (AB155933)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise