Rabbit Recombinant Monoclonal STAT1 antibody. Carrier free. Suitable for IP, ChIP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples.
Images
![ChIP - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (AB263866), expandable thumbnail](https://content.abcam.com/products/images/stat1-antibody-epr23049-111-bsa-and-azide-free-ab263866--chip-img120427.jpg/_jcr_content/renditions/webp-475.webp "ChIP - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (AB263866)")
![Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (AB263866), expandable thumbnail](https://content.abcam.com/products/images/stat1-antibody-epr23049-111-bsa-and-azide-free-ab263866--immunocytochemistry-immunofluorescence-img29631.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (AB263866)")
![Immunoprecipitation - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (AB263866), expandable thumbnail](https://content.abcam.com/products/images/stat1-antibody-epr23049-111-bsa-and-azide-free-ab263866--immunoprecipitation-img9068.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (AB263866)")
![Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (AB263866), expandable thumbnail](https://content.abcam.com/products/images/stat1-antibody-epr23049-111-bsa-and-azide-free-ab263866--immunocytochemistry-immunofluorescence-img91978.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (AB263866)")
![Flow Cytometry (Intracellular) - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (AB263866), expandable thumbnail](https://content.abcam.com/products/images/stat1-antibody-epr23049-111-bsa-and-azide-free-ab263866--flow-cytometry-intracellular-img415581.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (AB263866)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | ChIP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Expected | Tested | Tested | Not recommended | Tested | Expected |
| Mouse | Tested | Expected | Tested | Not recommended | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors (PubMed:12764129, PubMed:12855578, PubMed:15322115, PubMed:23940278, PubMed:34508746, PubMed:35568036, PubMed:9724754). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426, PubMed:35568036). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state (PubMed:28753426, PubMed:35568036). In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state (PubMed:8156998). Becomes activated in response to KITLG/SCF and KIT signaling (PubMed:15526160). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:19088846). Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylated at Thr-749 by IKBKB which promotes binding of STAT1 to the 5'-TTTGAGGC-3' sequence in the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 (PubMed:32209697). Phosphorylation at Thr-749 also promotes binding of STAT1 to the 5'-TTTGAGTC-3' sequence in the IL12B promoter and activation of IL12B transcription (PubMed:32209697). Involved in food tolerance in small intestine: associates with the Gasdermin-D, p13 cleavage product (13 kDa GSDMD) and promotes transcription of CIITA, inducing type 1 regulatory T (Tr1) cells in upper small intestine (By similarity).
Alternative names
Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1
Recommended products
Rabbit Recombinant Monoclonal STAT1 antibody. Carrier free. Suitable for IP, ChIP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23049-111
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab263866 is the carrier-free version of Anti-STAT1 antibody [EPR23049-111] - ChIP Grade ab239360.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Signal Transducer and Activator of Transcription 1 (STAT1) is a protein of about 91 kDa commonly known simply as STAT1. It belongs to the STAT protein family which is involved in gene regulation. STAT1 expresses widely in many tissues including the immune system where it plays a major role. As an important player in cellular responses to cytokines and growth factors STAT1 becomes activated through phosphorylation often referred to as phospho-STAT1. This phosphorylation promotes dimerization an important step for its function.
- Biological function summary
The STAT1 protein influences cell survival and immune responses. It functions as a transcription factor and participates in the interferon signaling pathway. In this setting it forms a complex with other STAT proteins such as STAT2 and STAT3 to regulate gene expression. By binding to specific DNA sequences in the nucleus STAT1 alters transcription in response to extracellular signals. It contributes to antiviral defense mechanisms and antiproliferative activities in various cells.
- Pathways
The STAT1 protein plays essential roles in the JAK-STAT signaling pathway and interferon signaling pathway. It operates closely with proteins such as JAK1 and TYK2 which are involved in the phosphorylation process activating STAT1. During this activation STAT1 helps initiate cellular responses to interferons a type of signaling protein released during viral infections. The JAK-STAT pathway facilitates various physiological processes including immune responses and cell growth regulation.
- Associated diseases and disorders
STAT1 dysregulation often connects to autoimmune diseases and infectious diseases. Elevated STAT1 activity associates with disorders like systemic lupus erythematosus where an imbalanced immune response occurs. Phosphorylation of STAT1 can also relate to chronic infections in cases where proper immune response activation becomes impaired. In certain cancers STAT1 interaction with proteins like SOCS1 might contribute to tumor immune evasion highlighting its vital role in pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![ChIP - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866), expandable thumbnail](https://content.abcam.com/products/images/stat1-antibody-epr23049-111-bsa-and-azide-free-ab263866--chip-img120427.jpg "ChIP - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)")
ChIP - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)
Chromatin was prepared from HT-1080 cells treated with IFN-γ (50ng/ml 30min) according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 μg of chromatin, 5 μg of Anti-STAT1 antibody [EPR23049-111] - ChIP Grade ab239360 (red), or 5 μg of rabbit normal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (gray) and 20 μl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
Primers and probes are commercial primers from Cell Signaling Technology (Cat. No.: #5148)
*http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT1 antibody [EPR23049-111] - ChIP Grade ab239360).
![Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866), expandable thumbnail](https://content.abcam.com/products/images/stat1-antibody-epr23049-111-bsa-and-azide-free-ab263866--immunocytochemistry-immunofluorescence-img29631.jpg "Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)")
Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling STAT1 with Anti-STAT1 antibody [EPR23049-111] - ChIP Grade ab239360 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1\1000 dilution (Green). Confocal image showing the signal translocate to nuclear in HeLa cells treated with IFN-γ (10ng/ml) for 30 mins. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT1 antibody [EPR23049-111] - ChIP Grade ab239360).
![Immunoprecipitation - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866), expandable thumbnail](https://content.abcam.com/products/images/stat1-antibody-epr23049-111-bsa-and-azide-free-ab263866--immunoprecipitation-img9068.jpg "Immunoprecipitation - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)")
Immunoprecipitation - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)
STAT1 was immunoprecipitated from 0.35mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with Anti-STAT1 antibody [EPR23049-111] - ChIP Grade ab239360 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-STAT1 antibody [EPR23049-111] - ChIP Grade ab239360 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate (10ug)
Lane 2: Anti-STAT1 antibody [EPR23049-111] - ChIP Grade ab239360 IP in RAW264.7 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-STAT1 antibody [EPR23049-111] - ChIP Grade ab239360 in RAW264.7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 75 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT1 antibody [EPR23049-111] - ChIP Grade ab239360).
All lanes: Immunoprecipitation - Anti-STAT1 antibody [EPR23049-111] - ChIP Grade (Anti-STAT1 antibody [EPR23049-111] - ChIP Grade ab239360)
Predicted band size: 87 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866), expandable thumbnail](https://content.abcam.com/products/images/stat1-antibody-epr23049-111-bsa-and-azide-free-ab263866--immunocytochemistry-immunofluorescence-img91978.jpg "Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)")
Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling STAT1 with Anti-STAT1 antibody [EPR23049-111] - ChIP Grade ab239360 at 1/50 dilution, followed byab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing the signal translocate to nuclear in RAW 264.7 cells treated with IFN-γ (10ng/ml) for 30 mins. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT1 antibody [EPR23049-111] - ChIP Grade ab239360).
![Flow Cytometry (Intracellular) - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866), expandable thumbnail](https://content.abcam.com/products/images/stat1-antibody-epr23049-111-bsa-and-azide-free-ab263866--flow-cytometry-intracellular-img415581.jpg "Flow Cytometry (Intracellular) - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)")
Flow Cytometry (Intracellular) - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling STAT1 with Anti-STAT1 antibody [EPR23049-111] - ChIP Grade ab239360 at 1/600 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)isotype control (black)and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT1 antibody [EPR23049-111] - ChIP Grade ab239360).
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