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Rabbit Recombinant Monoclonal STAT1 phospho S727 antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, Dot, WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 5 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free (AB215820), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free (AB215820), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free (AB215820), expandable thumbnail
  • Dot Blot - Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free (AB215820), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free (AB215820), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIC/CUT&RUN-seqDotWBICC/IFIHC-P
Human
Tested
Expected
Expected
Not recommended
Expected
Mouse
Predicted
Predicted
Expected
Not recommended
Predicted
Rat
Expected
Tested
Expected
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors (PubMed:12764129, PubMed:12855578, PubMed:15322115, PubMed:23940278, PubMed:34508746, PubMed:35568036, PubMed:9724754). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426, PubMed:35568036). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state (PubMed:28753426, PubMed:35568036). In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state (PubMed:8156998). Becomes activated in response to KITLG/SCF and KIT signaling (PubMed:15526160). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:19088846). Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylated at Thr-749 by IKBKB which promotes binding of STAT1 to the 5'-TTTGAGGC-3' sequence in the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 (PubMed:32209697). Phosphorylation at Thr-749 also promotes binding of STAT1 to the 5'-TTTGAGTC-3' sequence in the IL12B promoter and activation of IL12B transcription (PubMed:32209697). Involved in food tolerance in small intestine: associates with the Gasdermin-D, p13 cleavage product (13 kDa GSDMD) and promotes transcription of CIITA, inducing type 1 regulatory T (Tr1) cells in upper small intestine (By similarity).

Alternative names

Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1

Recommended products

Rabbit Recombinant Monoclonal STAT1 phospho S727 antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, Dot, WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR3146
Purification technique
Affinity purification Protein A
Specificity

A phospho specific peptide corresponding to residues surrounding Serine 727 of human Stat-1 was used as an immunogen. This antibody only detects Stat-1 phosphorylated at Serine 727.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab215820 is the carrier-free version of Anti-STAT1 (phospho S727) antibody [EPR3146] ab109461.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Signal Transducer and Activator of Transcription 1 (STAT1) is a protein of about 91 kDa commonly known simply as STAT1. It belongs to the STAT protein family which is involved in gene regulation. STAT1 expresses widely in many tissues including the immune system where it plays a major role. As an important player in cellular responses to cytokines and growth factors STAT1 becomes activated through phosphorylation often referred to as phospho-STAT1. This phosphorylation promotes dimerization an important step for its function.

Biological function summary

The STAT1 protein influences cell survival and immune responses. It functions as a transcription factor and participates in the interferon signaling pathway. In this setting it forms a complex with other STAT proteins such as STAT2 and STAT3 to regulate gene expression. By binding to specific DNA sequences in the nucleus STAT1 alters transcription in response to extracellular signals. It contributes to antiviral defense mechanisms and antiproliferative activities in various cells.

Pathways

The STAT1 protein plays essential roles in the JAK-STAT signaling pathway and interferon signaling pathway. It operates closely with proteins such as JAK1 and TYK2 which are involved in the phosphorylation process activating STAT1. During this activation STAT1 helps initiate cellular responses to interferons a type of signaling protein released during viral infections. The JAK-STAT pathway facilitates various physiological processes including immune responses and cell growth regulation.

Associated diseases and disorders

STAT1 dysregulation often connects to autoimmune diseases and infectious diseases. Elevated STAT1 activity associates with disorders like systemic lupus erythematosus where an imbalanced immune response occurs. Phosphorylation of STAT1 can also relate to chronic infections in cases where proper immune response activation becomes impaired. In certain cancers STAT1 interaction with proteins like SOCS1 might contribute to tumor immune evasion highlighting its vital role in pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free (ab215820), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free (ab215820)

    Immunohistochemical staining of paraffin embedded rat colon with purified Anti-STAT1 (phospho S727) antibody [EPR3146] ab109461 at a working dilution of 1/200. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT1 (phospho S727) antibody [EPR3146] ab109461).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free (ab215820), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free (ab215820)

    Immunohistochemical staining of paraffin embedded mouse colon with purified Anti-STAT1 (phospho S727) antibody [EPR3146] ab109461 at a working dilution of 1/200. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT1 (phospho S727) antibody [EPR3146] ab109461).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free (ab215820), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free (ab215820)

    Immunohistochemical staining of paraffin embedded human breast carcinoma with purified Anti-STAT1 (phospho S727) antibody [EPR3146] ab109461 at a working dilution of 1/200. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT1 (phospho S727) antibody [EPR3146] ab109461).

  • Dot Blot - Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free (ab215820), expandable thumbnail

    Dot Blot - Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free (ab215820)

    Dot Blot analysis of Lane 1: STAT1 (pS727) phospho peptide and Lane 2: STAT1 non-phospho peptide, labeling STAT1 (phospho S727) with Anti-STAT1 (phospho S727) antibody [EPR3146] ab109461 at 1/1000 dilution. 5% NFDM/TBST was used as the blocking and diluting buffer. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at 1/100000 dilution. Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT1 (phospho S727) antibody [EPR3146] ab109461).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free (ab215820), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free (ab215820)

    Unpurified Anti-STAT1 (phospho S727) antibody [EPR3146] ab109461, at a 1/100 dilution, staining STAT1 (phospho S727) in paraffin embedded Human stomach adenocarcinoma tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT1 (phospho S727) antibody [EPR3146] ab109461).

  • ChIC/CUT&RUN sequencing - Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free (ab215820), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free (ab215820)

    ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/µL, 2 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) treated with IFN gamma (50ng/ml 1h) cells and 5µg of Anti-STAT1 (phospho S727) antibody [EPR3146] ab109461 [EPR3146]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.

    Additional screenshots of mapped reads can be found in the Protocol booklet in the Support and downloads section.

    The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT1 (phospho S727) antibody [EPR3146] ab109461).

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