AB307836

Anti-STAT1 (phospho Y701) antibody [14/P-STAT1] - BSA and Azide free

Recombinant
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Mouse Recombinant Monoclonal STAT1 phospho Y701 antibody. Carrier free. Suitable for WB, Dot and reacts with Human, Synthetic peptide - Human samples.

View Alternative Names

Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1

2 Images

Supplier Data

Western blot - Anti-STAT1 (phospho Y701) antibody [14/P-STAT1] - BSA and Azide free (AB307836)

This image was produced using ab307835, the same clone but in a different formulation. In Western blot, Anti-STAT1 alpha antibody (ab92506) staining at 1/1000 dilution. Anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Blocking buffer : 5% NFDM/TBST. Exposure time : 48 seconds.

All lanes:

Western blot - Anti-STAT1 (phospho Y701) antibody [14/P-STAT1] (<a href='/en-us/products/primary-antibodies/stat1-phospho-y701-antibody-14-p-stat1-ab307835'>ab307835</a>) at 1/1000 dilution

Lane 1:

Untreated A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

A431 treated with 100ng/ml Epidermal Growth Factor (EGF) for 30 min whole cell lysate (untreated membrane) at 20 µg

Lane 3:

A431 treated with 100ng/ml Epidermal Growth Factor (EGF) for 30 min whole cell lysate (phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 87 kDa

false

Exposure time: 48s

Supplier Data

Dot Blot - Anti-STAT1 (phospho Y701) antibody [14/P-STAT1] - BSA and Azide free (AB307836)

This image was produced using ab307835, the same clone but in a different formulation. Dot blot analysis of STAT1 using ab307835 at 1 : 1000 followed by a Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1 : 5000 dilution. Exposure time : 180 seconds Blocking and diluting buffer and concentration : 5% NFDM/TBST Lane 1 : STAT1 (phospho Y701) peptide Lane 2 : STAT1 non-phospho peptide

Unconjugated

Anti-STAT1 (phospho Y701) antibody [14/P-STAT1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

14/P-STAT1

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Synthetic peptide - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Signal Transducer and Activator of Transcription 1 (STAT1) is a protein of about 91 kDa commonly known simply as STAT1. It belongs to the STAT protein family which is involved in gene regulation. STAT1 expresses widely in many tissues including the immune system where it plays a major role. As an important player in cellular responses to cytokines and growth factors STAT1 becomes activated through phosphorylation often referred to as phospho-STAT1. This phosphorylation promotes dimerization an important step for its function.

Biological function summary

The STAT1 protein influences cell survival and immune responses. It functions as a transcription factor and participates in the interferon signaling pathway. In this setting it forms a complex with other STAT proteins such as STAT2 and STAT3 to regulate gene expression. By binding to specific DNA sequences in the nucleus STAT1 alters transcription in response to extracellular signals. It contributes to antiviral defense mechanisms and antiproliferative activities in various cells.

Pathways

The STAT1 protein plays essential roles in the JAK-STAT signaling pathway and interferon signaling pathway. It operates closely with proteins such as JAK1 and TYK2 which are involved in the phosphorylation process activating STAT1. During this activation STAT1 helps initiate cellular responses to interferons a type of signaling protein released during viral infections. The JAK-STAT pathway facilitates various physiological processes including immune responses and cell growth regulation.

STAT1 dysregulation often connects to autoimmune diseases and infectious diseases. Elevated STAT1 activity associates with disorders like systemic lupus erythematosus where an imbalanced immune response occurs. Phosphorylation of STAT1 can also relate to chronic infections in cases where proper immune response activation becomes impaired. In certain cancers STAT1 interaction with proteins like SOCS1 might contribute to tumor immune evasion highlighting its vital role in pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors (PubMed : 12764129, PubMed : 12855578, PubMed : 15322115, PubMed : 23940278, PubMed : 34508746, PubMed : 35568036, PubMed : 9724754). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed : 28753426, PubMed : 35568036). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state (PubMed : 28753426, PubMed : 35568036). In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed : 26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state (PubMed : 8156998). Becomes activated in response to KITLG/SCF and KIT signaling (PubMed : 15526160). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 19088846). Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylated at Thr-749 by IKBKB which promotes binding of STAT1 to the 5'-TTTGAGGC-3' sequence in the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 (PubMed : 32209697). Phosphorylation at Thr-749 also promotes binding of STAT1 to the 5'-TTTGAGTC-3' sequence in the IL12B promoter and activation of IL12B transcription (PubMed : 32209697). Involved in food tolerance in small intestine : associates with the Gasdermin-D, p13 cleavage product (13 kDa GSDMD) and promotes transcription of CIITA, inducing type 1 regulatory T (Tr1) cells in upper small intestine (By similarity).

See full target information STAT1 phospho Y701

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-STAT1 (phospho Y701) antibody [14/P-STAT1] - BSA and Azide free

Western blot - Anti-STAT1 (phospho Y701) antibody [14/P-STAT1] - BSA and Azide free (AB307836)

Dot Blot - Anti-STAT1 (phospho Y701) antibody [14/P-STAT1] - BSA and Azide free (AB307836)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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