Anti-STAT2 antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody (AB233177)

Paraffin-embedded human stomach tissue stained for STAT2 using ab233177 at 20 μg/ml in immunohistochemical analysis. DAB staining.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody (AB233177)

Paraffin-embedded human kidney tissue stained for STAT2 using ab233177 at 20 μg/ml in immunohistochemical analysis. DAB staining.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody (AB233177)

Paraffin-embedded human stomach cancer tissue stained for STAT2 using ab233177 at 20 μg/ml in immunohistochemical analysis. DAB staining.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody (AB233177)

Formalin-fixed, paraffin-embedded human liver tissue stained for STAT2 using ab233177 at 20 μg/ml in immunohistochemical analysis. DAB staining.

• WB

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Western blot - Anti-STAT2 antibody (AB233177)

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Western blot - Anti-STAT2 antibody (ab233177) at 2 µg/mL

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HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 97 kDa

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• WB

Supplier Data

Western blot - Anti-STAT2 antibody (AB233177)

All lanes:

Western blot - Anti-STAT2 antibody (ab233177) at 2 µg/mL

All lanes:

Pig liver tissue lysate

Predicted band size: 97 kDa

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• WB

Supplier Data

Western blot - Anti-STAT2 antibody (AB233177)

All lanes:

Western blot - Anti-STAT2 antibody (ab233177) at 2 µg/mL

All lanes:

K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate

Predicted band size: 97 kDa

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• WB

Supplier Data

Western blot - Anti-STAT2 antibody (AB233177)

All lanes:

Western blot - Anti-STAT2 antibody (ab233177) at 2 µg/mL

All lanes:

Recombinant human STAT2 protein

Predicted band size: 97 kDa

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• WB

CiteAb

Western blot - Anti-STAT2 antibody (AB233177)

STAT2 western blot using anti-STAT2 antibody ab233177. Publication image and figure legend from Cao, H., Gao, R., et al., 2020, FEBS Open Bio, PubMed 31981447.

ab233177 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab233177 please see the product overview.

TRIM66 regulated STAT2 expression. (A) The correlation of TRIM66 and STAT2 in patients with prostate cancer was analyzed in TCGA prostate cancer dataset. (B) The mRNA expression of STAT2 in PC-3 cells transfected with TRIM66 shRNA was determined by RT-qPCR. (C) The protein expression of STAT2 and phosphorylated (p)-STAT2 in PC-3 cells transfected with TRIM66 shRNA was analyzed using western blot. (D) Luciferase reporter assay of STAT2 promoter in cells transfected with vector or shTRIM66. (E) The mRNA levels of STAT2 in PC-3 cells transfected with shSTAT2 or corresponding vector were confirmed by RT-qPCR. (F) Cell viabilities of PC-3 cells transfected with shSTAT2 or corresponding vector were determined by cell count assay. (G) Fluorescence-activated cell sorting analysis of the effects of STAT2 depletion on the cell cycle in PC-3 cells. (H) Transwell of migration and invasion assay of PC-3 cells transfected with shSTAT2 and corresponding vector. (I) The expression of EMT markers in PC-3 cells transfected with shSTAT2 and corresponding vector was determined by western blot. All of the experiments were biologically repeated at least three times. Data were expressed as mean ± SD and analyzed by Student's t-test. **p < 0.01, ***p < 0.001.

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