Skip to main content

Rabbit Recombinant Monoclonal STAT2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Sandwich ELISA - Anti-STAT2 antibody [EPR29255-522] - BSA and Azide free (Detector) (AB322478), expandable thumbnail
  • Sandwich ELISA - Anti-STAT2 antibody [EPR29255-522] - BSA and Azide free (Detector) (AB322478), expandable thumbnail
  • Sandwich ELISA - Anti-STAT2 antibody [EPR29255-522] - BSA and Azide free (Detector) (AB322478), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Signal transducer and activator of transcription that mediates signaling by type I interferons (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state (PubMed:9020188, PubMed:23391734). In addition, has also a negative feedback regulatory role in the type I interferon signaling by recruiting USP18 to the type I IFN receptor subunit IFNAR2 thereby mitigating the response to type I IFNs (PubMed:28165510). Acts as a regulator of mitochondrial fission by modulating the phosphorylation of DNM1L at 'Ser-616' and 'Ser-637' which activate and inactivate the GTPase activity of DNM1L respectively (PubMed:26122121, PubMed:23391734, PubMed:9020188).

Alternative names

Signal transducer and activator of transcription 2, p113, STAT2

Rabbit Recombinant Monoclonal STAT2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Alternative names

Signal transducer and activator of transcription 2, p113, STAT2

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR29255-522

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab322478 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human STAT2. The recommended pair for sandwich ELISA is:
Capture: Anti-STAT2 antibody [EPR29255-538] - BSA and Azide free (Capture) ab322477, Human STAT2 Capture Antibody (unconjugated)
Detector: ab322478, Human STAT2 Detector Antibody (unconjugated)
The reference range value is 7.81-500 pg/mL.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Anti-STAT2 antibody [EPR29255-522] - BSA and Azide free (Detector) (ab322478), expandable thumbnail

    Sandwich ELISA - Anti-STAT2 antibody [EPR29255-522] - BSA and Azide free (Detector) (ab322478)

    Sandwich ELISA of Human STAT2 Antibody Pair - BSA and Azide free ab322476 with the capture antibody Anti-STAT2 antibody [EPR29255-538] - BSA and Azide free (Capture) ab322477 dilution at 2 ug/mL and detector antibody ab322478 dilution at 0.5 ug/mL.

  • Sandwich ELISA - Anti-STAT2 antibody [EPR29255-522] - BSA and Azide free (Detector) (ab322478), expandable thumbnail

    Sandwich ELISA - Anti-STAT2 antibody [EPR29255-522] - BSA and Azide free (Detector) (ab322478)

    Interpolated concentration of native STAT2 was measured at different sample concentrations. Undiluted samples are as follows: Hela cell extract (500 µg/mL) and K562 cell extract (1000 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (Antibody Diluent CP2 ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Anti-STAT2 antibody [EPR29255-522] - BSA and Azide free (Detector) (ab322478), expandable thumbnail

    Sandwich ELISA - Anti-STAT2 antibody [EPR29255-522] - BSA and Azide free (Detector) (ab322478)

    Interpolated concentrations of native STAT2 in human control wild type HeLa (human cervical adenocarcinoma epithelial cell) and STAT2 knockout HeLa cell based on 500 µg/mL extract loads. The concentrations of STAT2 were measured in duplicate and interpolated from the STAT2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean STAT2 concentration was determined to be 105.9 pg/mL in wild type HeLa extract (Human wild-type HeLa cell line Human wild-type HeLa cell line ab255928) and undetectable in STAT2 knockout HeLa extract (Human STAT2 knockout HeLa cell line Human STAT2 knockout HeLa cell line ab261819).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com