JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB53132

Anti-STAT2 (phospho Y690) antibody

4

(2 Reviews)

|

(29 Publications)

Rabbit Polyclonal STAT2 phospho Y690 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 29 publications. Immunogen corresponding to Synthetic Peptide within Human STAT2 pY690 aa 650-750.

View Alternative Names

Signal transducer and activator of transcription 2, p113, STAT2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 (phospho Y690) antibody (AB53132)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 (phospho Y690) antibody (AB53132)

ab53132, at 1/50 dilution, staining STAT2 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.

Western blot - Anti-STAT2 (phospho Y690) antibody (AB53132)
  • WB

Unknown

Western blot - Anti-STAT2 (phospho Y690) antibody (AB53132)

All lanes:

Western blot - Anti-STAT2 (phospho Y690) antibody (ab53132) at 1/300 dilution

Lane 1:

HeLa cell extract treated with IFN (2500U/ml, 30mins)

Lane 2:

HeLa cell extract treated with IFN (2500U/ml, 30mins) with immunising peptide

Predicted band size: 97 kDa

Observed band size: 98 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human STAT2 pY690 aa 650-750. The exact immunogen used to generate this antibody is proprietary information.

P52630

style
left-column

Alternative Products

Primary Antibodies

AB245394

Anti-STAT2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody (AB245394)

  • 0
  • 0
Primary Antibodies

AB124283

Anti-STAT2 antibody

Immunoprecipitation - Anti-STAT2 antibody (AB124283)

  • 0
  • 0
Primary Antibodies

AB233177

Anti-STAT2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody (AB233177)

  • 2
  • 1
Primary Antibodies

AB32367

Anti-STAT2 antibody [Y141]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-STAT2 antibody [Y141] (AB32367)

  • 5
  • 1
Primary Antibodies

AB239811

Anti-STAT2 antibody [Y141] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-STAT2 antibody [Y141] - BSA and Azide free (AB239811)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB109461

Anti-STAT1 (phospho S727) antibody [EPR3146]

20ul selling size|Recombinant|Advanced Validation|RabMAb|KO Validated

Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)

  • 4
  • 1
Primary Antibodies

AB303500

Anti-TYK2 antibody [EPR24628-106]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-TYK2 antibody [EPR24628-106] (AB303500)

  • 0
  • 0
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab53132 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Signal Transducer and Activator of Transcription 2 commonly known as STAT2 is a protein with a molecular mass of approximately 97 kDa. It functions mainly as a transcription factor and is an essential mediator of the interferon (IFN) signaling pathway. STAT2 is expressed in various tissues including immune cells where it responds to external stimuli. It works by translocating to the nucleus upon phosphorylation subsequently influencing the expression of genes involved in immune response.
Biological function summary

Members of the STAT protein family including STAT2 play a central role in mediating immune responses. STAT2 often forms a complex with STAT1 and other proteins such as IRF9 to initiate the transcription of interferon-stimulated genes (ISGs). This complex known as the ISGF3 complex facilitates the cellular response to viral infections by promoting the expression of antiviral proteins that help modify the host cellular environment to resist viral replication.

Pathways

The involvement of STAT2 is closely associated with the JAK-STAT signaling pathway and the interferon signaling pathway. In these pathways it interacts with proteins like JAK1 TYK2 and STAT1 playing a part in transmitting signals from the cell surface to the nucleus. Such pathways are fundamental to regulating the body's immune response to pathogens and managing cell growth and apoptosis in various cells.

STAT2 alterations have been linked to autoimmune diseases and viral infections. Disruptions in STAT2 function can impair the body's ability to combat viruses effectively making individuals more susceptible to infections. STAT2 mutations or dysregulations might also connect to disorders like Systemic Lupus Erythematosus where immune system malfunctions occur. The interactions of STAT2 with other proteins like STAT1 and IRF9 in disease contexts underline its importance in maintaining immune system balance.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Signal transducer and activator of transcription that mediates signaling by type I interferons (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state (PubMed : 23391734, PubMed : 9020188). In addition, also has a negative feedback regulatory role in the type I interferon signaling by recruiting USP18 to the type I IFN receptor subunit IFNAR2 thereby mitigating the response to type I IFNs (PubMed : 28165510). Acts as a regulator of mitochondrial fission by modulating the phosphorylation of DNM1L at 'Ser-616' and 'Ser-637' which activate and inactivate the GTPase activity of DNM1L respectively (PubMed : 23391734, PubMed : 26122121, PubMed : 9020188).
See full target information STAT2 pY690
style
left-column

Publications (29)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 13:5218-5235 PubMed38058799

2023

TP53TG1/STAT axis is involved in the development of colon cancer through affecting PD-L1 expression and immune escape mechanism of tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wenmin Ji,Wenyan Wang,Yanfang Wei

EBioMedicine 86:104312 PubMed36335669

2022

Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shihui Xu,Xiaobing Yang,Qingzhou Chen,Zhuoliang Liu,Ying Chen,Xiaotian Yao,An Xiao,Jianwei Tian,Liling Xie,Miaomiao Zhou,Zheng Hu,Fengxin Zhu,Xin Xu,Fanfan Hou,Jing Nie

Cancer communications (London, England) 42:245-265 PubMed35234370

2022

The Fibrillin-1/VEGFR2/STAT2 signaling axis promotes chemoresistance via modulating glycolysis and angiogenesis in ovarian cancer organoids and cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ziliang Wang,Wei Chen,Ling Zuo,Midie Xu,Yong Wu,Jiami Huang,Xu Zhang,Yongheng Li,Jing Wang,Jing Chen,Husheng Wang,Huizhen Sun

PLoS pathogens 17:e1009860 PubMed34432853

2021

Robust innate immune responses at the placenta during early gestation may limit in utero HIV transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Erica L Johnson,Dominika Swieboda,Amanda Olivier,Elizabeth Ann L Enninga,Rana Chakraborty

Experimental and therapeutic medicine 22:875 PubMed34194553

2021

Curcumin inhibits the viability, migration and invasion of papillary thyroid cancer cells by regulating the miR-301a-3p/STAT3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liang,Deyu Kong,Yi Zhang,Siqi Li,Yan Li,Liying Dong,Ningxin Zhang,Junfeng Ma

Oncology reports 45:217-229 PubMed33200228

2020

Combination of Huanglian Jiedu Decoction and erlotinib delays growth and improves sensitivity of EGFR‑mutated NSCLC cells in vitro and in vivo via STAT3/Bcl‑2 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowen Zhou,Bojia Liu,Qing Ning,Zhi Xia,Rongling Zhong,Li Zhang,Lei Wu

EMBO reports 21:e50051 PubMed32715615

2020

Autoimmunity gene IRGM suppresses cGAS-STING and RIG-I-MAVS signaling to control interferon response.

Applications

Unspecified application

Species

Unspecified reactive species

Kautilya Kumar Jena,Subhash Mehto,Parej Nath,Nishant Ranjan Chauhan,Rinku Sahu,Kollori Dhar,Saroj Kumar Das,Srinivasa Prasad Kolapalli,Krushna C Murmu,Ashish Jain,Sivaram Krishna,Bhabani Sankar Sahoo,Soma Chattopadhyay,Tor Erik Rusten,Punit Prasad,Swati Chauhan,Santosh Chauhan

Acta neuropathologica 140:121-142 PubMed32562018

2020

Congenic expression of poly-GA but not poly-PR in mice triggers selective neuron loss and interferon responses found in C9orf72 ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine D LaClair,Qihui Zhou,Meike Michaelsen,Benedikt Wefers,Monika S Brill,Aleksandar Janjic,Birgit Rathkolb,Daniel Farny,Mikolaj Cygan,Martin Hrabe de Angelis,Wolfgang Wurst,Manuela Neumann,Wolfgang Enard,Thomas Misgeld,Thomas Arzberger,Dieter Edbauer

Cell death and differentiation 27:71-84 PubMed31076632

2019

Global deletion of Optineurin results in altered type I IFN signaling and abnormal bone remodeling in a model of Paget's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sing-Wai Wong,Bo-Wen Huang,Xiangxiang Hu,Eui Ho Kim,Joseph P Kolb,Ricardo J Padilla,Peng Xue,Lufei Wang,Thomas H Oguin,Patricia A Miguez,Henry C Tseng,Ching-Chang Ko,Jennifer Martinez

Cell biology international 43:605-612 PubMed30958598

2019

Role of autophagy in inhibiting the proliferation of A549 cells by type III interferon.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Gao,Xiaoqin Yu,Bo Zhang,Min Kong,Yong Fang,Yu Cai,Chengchu Zhu,Jun Zhao,Jicheng Li
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com