AB277618

Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal STAT2 phospho Y690 antibody. Carrier free. Suitable for IP, ChIP, Dot, WB and reacts with Human, Synthetic peptide samples.

View Alternative Names

Signal transducer and activator of transcription 2, p113, STAT2

4 Images

ChIP - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade - BSA and Azide free (AB277618)

ChIP

Lab

ChIP - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade - BSA and Azide free (AB277618)

This data was developed using ab191601, the same antibody clone in a different buffer formulation.

Chromatin was prepared from A431(human epidermoid carcinoma cell line)(starved overnight, treated with IFN-α (10ng/ml 0.5 h)) and A431 (starved overnight) (non-treated) according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 μg of chromatin, 5 μg of ab191601 (Red), or 5 μg of rabbit normal IgG ab172730 (Gray) and 25 μl of Protein A/G Dynabeads. The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

Primers are from paper PMID : 30240626.

*https : //www.abcam.com/resources?keywords=X%20ChIP%20protocol

Unknown

Immunoprecipitation - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade - BSA and Azide free (AB277618)

This data was developed using ab191601, the same antibody clone in a different buffer formulation.

STAT2 (phospho Y690) was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia) (treated with 2500 U/ml IFN alpha for 30 mins) whole cell lysate with ab191601 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab191601 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/1000 dilution.

Lane 1 : K-562 (human chronic myelogenous leukemia) (treated as above) whole cell lysate 10 ug

Lane 2 : ab191601 IP in K-562 (treated as above) whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab191601 in K-562 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade (<a href='/en-us/products/primary-antibodies/stat2-phospho-y690-antibody-epr18549-42-chip-grade-ab191601'>ab191601</a>)

Predicted band size: 97 kDa

Observed band size: 97 kDa

false

Lab

Western blot - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade - BSA and Azide free (AB277618)

This data was developed using ab191601, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 123 seconds.

All lanes:

Western blot - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade (<a href='/en-us/products/primary-antibodies/stat2-phospho-y690-antibody-epr18549-42-chip-grade-ab191601'>ab191601</a>) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma epithelial cell) (starved overnight, then 10 ng/ml IFN-alpha treated for 30 minutes), whole cell lysate at 20 µg

Lane 2:

A431 (starved overnight), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 97 kDa

Observed band size: 113 kDa,97 kDa

false

Unknown

Dot Blot - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade - BSA and Azide free (AB277618)

This data was developed using ab191601, the same antibody clone in a different buffer formulation.

Dot blot analysis of STAT2 (phospho Y690) using ab191601 at 1/1000 dilution (1.4 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

Lane 1 : STAT2 (phospho Y690) phospho peptide
Lane 2 : STAT2 non-phospho peptide

Exposure time : 30 seconds.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18549-42

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Dot, WB, ChIP, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab277618 is the carrier-free version of ab191601.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Signal Transducer and Activator of Transcription 2 commonly known as STAT2 is a protein with a molecular mass of approximately 97 kDa. It functions mainly as a transcription factor and is an essential mediator of the interferon (IFN) signaling pathway. STAT2 is expressed in various tissues including immune cells where it responds to external stimuli. It works by translocating to the nucleus upon phosphorylation subsequently influencing the expression of genes involved in immune response.

Biological function summary

Members of the STAT protein family including STAT2 play a central role in mediating immune responses. STAT2 often forms a complex with STAT1 and other proteins such as IRF9 to initiate the transcription of interferon-stimulated genes (ISGs). This complex known as the ISGF3 complex facilitates the cellular response to viral infections by promoting the expression of antiviral proteins that help modify the host cellular environment to resist viral replication.

Pathways

The involvement of STAT2 is closely associated with the JAK-STAT signaling pathway and the interferon signaling pathway. In these pathways it interacts with proteins like JAK1 TYK2 and STAT1 playing a part in transmitting signals from the cell surface to the nucleus. Such pathways are fundamental to regulating the body's immune response to pathogens and managing cell growth and apoptosis in various cells.

STAT2 alterations have been linked to autoimmune diseases and viral infections. Disruptions in STAT2 function can impair the body's ability to combat viruses effectively making individuals more susceptible to infections. STAT2 mutations or dysregulations might also connect to disorders like Systemic Lupus Erythematosus where immune system malfunctions occur. The interactions of STAT2 with other proteins like STAT1 and IRF9 in disease contexts underline its importance in maintaining immune system balance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Signal transducer and activator of transcription that mediates signaling by type I interferons (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state (PubMed : 23391734, PubMed : 9020188). In addition, has also a negative feedback regulatory role in the type I interferon signaling by recruiting USP18 to the type I IFN receptor subunit IFNAR2 thereby mitigating the response to type I IFNs (PubMed : 28165510). Acts as a regulator of mitochondrial fission by modulating the phosphorylation of DNM1L at 'Ser-616' and 'Ser-637' which activate and inactivate the GTPase activity of DNM1L respectively (PubMed : 23391734, PubMed : 26122121, PubMed : 9020188).

See full target information STAT2 phospho Y690

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade - BSA and Azide free

ChIP - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade - BSA and Azide free (AB277618)

Immunoprecipitation - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade - BSA and Azide free (AB277618)

Western blot - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade - BSA and Azide free (AB277618)

Dot Blot - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade - BSA and Azide free (AB277618)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Sample Prep & Detection Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise