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AB226942

Anti-STAT3 antibody

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(6 Publications)

Rabbit Polyclonal STAT3 antibody. Suitable for WB, ICC/IF and reacts with Zebrafish, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human STAT3.

View Alternative Names

APRF, STAT3, Signal transducer and activator of transcription 3, Acute-phase response factor

4 Images
Western blot - Anti-STAT3 antibody (AB226942)
  • WB

Supplier Data

Western blot - Anti-STAT3 antibody (AB226942)

Samples were separated by 7.5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-STAT3 antibody (ab226942) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 88 kDa

false

Western blot - Anti-STAT3 antibody (AB226942)
  • WB

Supplier Data

Western blot - Anti-STAT3 antibody (AB226942)

All lanes:

Western blot - Anti-STAT3 antibody (ab226942) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

HeLa whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Secondary

All lanes:

Goat Anti Rabbit IgG (HRP)

Predicted band size: 88 kDa

false

Western blot - Anti-STAT3 antibody (AB226942)
  • WB

Supplier Data

Western blot - Anti-STAT3 antibody (AB226942)

All lanes:

Western blot - Anti-STAT3 antibody (ab226942) at 1/1000 dilution

Lane 1:

Wild Type (WT) HeLa cell extract at 30 µg

Lane 2:

STAT3 knockout (KO) HeLa cell extract at 30 µg

Secondary

All lanes:

Goat anti rabbit IgG (HRP)

Predicted band size: 88 kDa

false

Western blot - Anti-STAT3 antibody (AB226942)
  • WB

Supplier Data

Western blot - Anti-STAT3 antibody (AB226942)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-STAT3 antibody (ab226942) at 1/1000 dilution

Lane 1:

Zebrafish brain whole tissue lysate at 30 µg

Lane 2:

Zebrafish eye whole tissue lysate at 30 µg

Lane 3:

Whole zebrafish tissue lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 88 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Zebrafish

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human STAT3. The exact immunogen used to generate this antibody is proprietary information.

P40763

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Signal Transducer and Activator of Transcription 3 (STAT3) also called p-stat3 or phospho-STAT3 is a critical transcription factor involved in various cellular processes. The molecular weight of STAT3 is approximately 92 kDa. This protein is expressed broadly across many tissues and cell types. Mechanically upon cytokine or growth factor stimulation STAT3 undergoes phosphorylation resulting in dimerization. This phosphorylated form often referred to as phospho-Stat3 or p-STAT3 translocates to the nucleus where it binds specific DNA sequences to modulate gene transcription.
Biological function summary

STAT3 is an important player in regulating cell growth and apoptosis. It functions not only as a transcription factor but also as part of a larger protein complex that operates within the cell nucleus to influence gene expression. This multifaceted role enables STAT3 to control the expression of genes related to cell proliferation survival and differentiation impacting various biological processes. These functions highlight its importance in tissue homeostasis and response to extracellular signals.

Pathways

STAT3 is actively involved in the JAK-STAT signaling pathway a principal route for many cytokines and growth factors and the MAPK pathway. STAT3 interacts with proteins such as JAK kinases which phosphorylate Stat3 and initiate the STAT3 signaling cascade. Additionally it closely associates with the protein Raf1 in the MAPK pathway linking external signals to transcriptional responses. These pathways play a significant role in immune response inflammation and growth signaling.

Researchers have implicated STAT3 in cancer and autoimmune diseases. Persistent activation of STAT3 is often observed in various cancers including breast and lung cancer promoting oncogenesis through altered gene expression. Additionally abnormal STAT3 signaling is associated with autoimmune conditions such as rheumatoid arthritis. The interplay between STAT3 and proteins like IL-6 in these diseases highlights its potential as a therapeutic target for intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed : 10688651, PubMed : 12359225, PubMed : 12873986, PubMed : 15194700, PubMed : 15653507, PubMed : 16285960, PubMed : 17344214, PubMed : 18242580, PubMed : 18782771, PubMed : 22306293, PubMed : 23084476, PubMed : 28262505, PubMed : 32929201, PubMed : 38404237). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed : 15653507, PubMed : 16285960, PubMed : 17344214, PubMed : 18782771, PubMed : 28262505, PubMed : 32929201). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 12873986). Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes (PubMed : 12359225). Activated by IL31 through IL31RA (PubMed : 15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg) : acetylation promotes its transcription activity and cell differentiation while deacetylation and oxidation of lysine residues by LOXL3 inhibits differentiation (PubMed : 28065600, PubMed : 28262505). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed : 17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed : 18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed : 23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity). Following JAK/STAT signaling activation and as part of a complex with NFATC3 and NFATC4, binds to the alpha-beta E4 promoter region of CRYAB and activates transcription in cardiomyocytes (By similarity).
See full target information STAT3
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Circulation 151:299-317 PubMed39429196

2024

Arterial-Lymphatic-Like Endothelial Cells Appear in Hereditary Hemorrhagic Telangiectasia 2 and Contribute to Vascular Leakage and Arteriovenous Malformations.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Xiuju Wu,Yan Zhao,Daoqin Zhang,Li Zhang,Xinjiang Cai,Jaden Ji,Zheng Jing,Kristina I Boström,Yucheng Yao

Cell discovery 9:33 PubMed36977660

2023

Nuclear PD-L1 promotes EGR1-mediated angiogenesis and accelerates tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Yu,Ai Zhuang,Xiang Gu,Yu Hua,Ludi Yang,Shengfang Ge,Jing Ruan,Peiwei Chai,Renbing Jia,Xianqun Fan

BMC nephrology 23:413 PubMed36575400

2022

Ntrk1 promotes mesangial cell proliferation and inflammation in rat glomerulonephritis model by activating the STAT3 and p38/ERK MAPK signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Xiongjun Dong,Yingchun Tang

Cell death & disease 13:417 PubMed35487888

2022

ICAM-1 promotes cancer progression by regulating SRC activity as an adapter protein in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Ji Lim,Jae-Hyeok Kang,Yeon-Ju Kim,Seungmo Kim,Su-Jae Lee

Experimental and therapeutic medicine 23:97 PubMed34976139

2022

MicroRNA-141-3p inhibits the progression of oral squamous cell carcinoma via targeting PBX1 through the JAK2/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mingguo Cao,Kebin Tian,Weifeng Sun,Jun Xu,Yu Tang,Shilian Wu

BMC pulmonary medicine 21:50 PubMed33535997

2021

LncRNA LINC01116 sponges miR-93-5p to promote cell invasion and migration in small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenzhou Liu,Feihai Liang,Guangyu Yang,Lei Xian
View all publications
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