Anti-STAT3 Antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 Antibody (AB327249)

Immunohistochemical staining of human skeletal muscle using ab327249 at 1 : 50–1 : 200 dilution, showing weak nuclear positivity in myocytes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 Antibody (AB327249)

Immunohistochemical staining of human cervix, uterine using ab327249 at 1 : 50–1 : 200 dilution, showing moderate nuclear positivity in squamous epithelial cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 Antibody (AB327249)

Immunohistochemical staining of human tonsil using ab327249 at 1 : 50–1 : 200 dilution, showing strong cytoplasmic positivity in squamous epithelial cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 Antibody (AB327249)

Immunohistochemical staining of human fallopian tube using ab327249 at 1 : 50–1 : 200 dilution, showing moderate cytoplasmic and nuclear positivity in glandular cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 Antibody (AB327249)

Immunohistochemical staining of human spleen using ab327249 at 1 : 50–1 : 200 dilution, showing moderate cytoplasmic positivity in cells in red pulp.

• ChIP

Supplier Data

ChIP - Anti-STAT3 Antibody (AB327249)

ChIP-Exo-Seq composite graph for ab327249 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

Supplier Data

Western blot - Anti-STAT3 Antibody (AB327249)

Western blot analysis in human cell lines A-549 and PC-3 using ab327249 at 0.04–0.4 µg/ml. Corresponding STAT3 RNA-seq data are presented for the same cell lines. Loading control : Anti-PPIB antibody.

All lanes:

Western blot - Anti-STAT3 Antibody (ab327249)

Lane 2:

Human cell lines A-549

Lane 3:

PC-3

Predicted band size: 88.07 kDa

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