Rabbit Recombinant Monoclonal STAT3 phospho S727 antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, IHC-P, Dot, WB, ICC/IF, IP and reacts with Human, Rat, Mouse, Recombinant full length protein - Human samples. Cited in 1 publication.
Images
![Dot Blot - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (AB219593), expandable thumbnail](https://content.abcam.com/products/images/stat3-phospho-s727-antibody-e121-31-bsa-and-azide-free-ab219593--dot-blot-img6026.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (AB219593)")
![Immunocytochemistry/ Immunofluorescence - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (AB219593), expandable thumbnail](https://content.abcam.com/products/images/stat3-phospho-s727-antibody-e121-31-bsa-and-azide-free-ab219593--immunocytochemistry-immunofluorescence-img120133.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (AB219593)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (AB219593), expandable thumbnail](https://content.abcam.com/products/images/stat3-phospho-s727-antibody-e121-31-bsa-and-azide-free-ab219593--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74205.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (AB219593)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (AB219593), expandable thumbnail](https://content.abcam.com/products/images/stat3-phospho-s727-antibody-e121-31-bsa-and-azide-free-ab219593--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66943.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (AB219593)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (AB219593), expandable thumbnail](https://content.abcam.com/products/images/stat3-phospho-s727-antibody-e121-31-bsa-and-azide-free-ab219593--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32899.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (AB219593)")
Publications
Filter by
- Experimental and therapeutic medicine 14:3583-35882017Protective effect of ulinastatin on severe pulmonary infection under immunosuppression and its molecular mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenshuai Liu et. al.PubMed 29042952
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ChIC/CUT&RUN-seq | IHC-P | Dot | WB | ICC/IF | IP | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected | Tested | Expected | Expected |
| Cow | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Macaque monkey | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
ChIC/CUT&RUN-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Cow, Macaque monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes For unpurified, use 1/50. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Cow, Macaque monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Cow, Macaque monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls. |
Species Rat | Dilution info - | Notes Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls. |
Species Human | Dilution info - | Notes Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Cow, Macaque monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Cow, Macaque monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Cow, Macaque monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
4 products for Alternative Version
Target data
Function
Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194700, PubMed:15653507, PubMed:16285960, PubMed:17344214, PubMed:18242580, PubMed:18782771, PubMed:22306293, PubMed:23084476, PubMed:28262505, PubMed:32929201, PubMed:38404237). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:15653507, PubMed:16285960, PubMed:17344214, PubMed:18782771, PubMed:28262505, PubMed:32929201). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:12873986). Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes (PubMed:12359225). Activated by IL31 through IL31RA (PubMed:15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg): acetylation promotes its transcription activity and cell differentiation while deacetylation and oxidation of lysine residues by LOXL3 inhibits differentiation (PubMed:28065600, PubMed:28262505). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed:23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity). Following JAK/STAT signaling activation and as part of a complex with NFATC3 and NFATC4, binds to the alpha-beta E4 promoter region of CRYAB and activates transcription in cardiomyocytes (By similarity).
Alternative names
APRF, STAT3, Signal transducer and activator of transcription 3, Acute-phase response factor
Recommended products
Rabbit Recombinant Monoclonal STAT3 phospho S727 antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, IHC-P, Dot, WB, ICC/IF, IP and reacts with Human, Rat, Mouse, Recombinant full length protein - Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- E121-31
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab219593 is the carrier-free version of Anti-STAT3 (phospho S727) antibody [E121-31] ab32143.
Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Signal Transducer and Activator of Transcription 3 (STAT3) also called p-stat3 or phospho-STAT3 is a critical transcription factor involved in various cellular processes. The molecular weight of STAT3 is approximately 92 kDa. This protein is expressed broadly across many tissues and cell types. Mechanically upon cytokine or growth factor stimulation STAT3 undergoes phosphorylation resulting in dimerization. This phosphorylated form often referred to as phospho-Stat3 or p-STAT3 translocates to the nucleus where it binds specific DNA sequences to modulate gene transcription.
- Biological function summary
STAT3 is an important player in regulating cell growth and apoptosis. It functions not only as a transcription factor but also as part of a larger protein complex that operates within the cell nucleus to influence gene expression. This multifaceted role enables STAT3 to control the expression of genes related to cell proliferation survival and differentiation impacting various biological processes. These functions highlight its importance in tissue homeostasis and response to extracellular signals.
- Pathways
STAT3 is actively involved in the JAK-STAT signaling pathway a principal route for many cytokines and growth factors and the MAPK pathway. STAT3 interacts with proteins such as JAK kinases which phosphorylate Stat3 and initiate the STAT3 signaling cascade. Additionally it closely associates with the protein Raf1 in the MAPK pathway linking external signals to transcriptional responses. These pathways play a significant role in immune response inflammation and growth signaling.
- Associated diseases and disorders
Researchers have implicated STAT3 in cancer and autoimmune diseases. Persistent activation of STAT3 is often observed in various cancers including breast and lung cancer promoting oncogenesis through altered gene expression. Additionally abnormal STAT3 signaling is associated with autoimmune conditions such as rheumatoid arthritis. The interplay between STAT3 and proteins like IL-6 in these diseases highlights its potential as a therapeutic target for intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Dot Blot - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593), expandable thumbnail](https://content.abcam.com/products/images/stat3-phospho-s727-antibody-e121-31-bsa-and-azide-free-ab219593--dot-blot-img6026.jpg "Dot Blot - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)")
Dot Blot - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)
Dot Blot analysis of Lane 1: STAT3 (pS727) phospho peptide and Lane 2: STAT3 non-phospho peptide labeling STAT3 (phospho S727) with Anti-STAT3 (phospho S727) antibody [E121-31] ab32143 at 1/1000 dilution (0.009 μg/ml). 5% NFDM /TBST was used as the diluting and blocking buffer and concentration. Goat Anti-Rabbit IgG H&L (HRP) ab97051, Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100,000 dilution. Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT3 (phospho S727) antibody [E121-31] ab32143).
![Immunocytochemistry/ Immunofluorescence - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593), expandable thumbnail](https://content.abcam.com/products/images/stat3-phospho-s727-antibody-e121-31-bsa-and-azide-free-ab219593--immunocytochemistry-immunofluorescence-img120133.jpg "Immunocytochemistry/ Immunofluorescence - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)")
Immunocytochemistry/ Immunofluorescence - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)
Immunocytochemical/Immunofluorescence analysis of untreated, EGF treated and EGF + LP treated A431 cells labelling STAT3 (phospho S727) with Anti-STAT3 (phospho S727) antibody [E121-31] ab32143 (left) and STAT3 with Anti-STAT3 antibody [E121-21] ab32500 (right) both at a dilution of 1/500.
Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/1000) was used as the secondary antibody (green). DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889)(1/200) was used as a counterstain (red).
The green staining was increased and translocated from the cytoplasm into the nucleus in the EGF (Recombinant human EGF protein (Animal Free) ab9697 100ng/ml, 10min) treated A431 cells when compared with A431 cells without treatment. After LP treatment, the green signal was decreased. For the pan antibody, there was no great difference after EGF (100ng/ml, 10min) or EGF (100ng/ml, 10min) + LP treatment.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT3 (phospho S727) antibody [E121-31] ab32143).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593), expandable thumbnail](https://content.abcam.com/products/images/stat3-phospho-s727-antibody-e121-31-bsa-and-azide-free-ab219593--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74205.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)
Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified Anti-STAT3 (phospho S727) antibody [E121-31] ab32143 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT3 (phospho S727) antibody [E121-31] ab32143).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593), expandable thumbnail](https://content.abcam.com/products/images/stat3-phospho-s727-antibody-e121-31-bsa-and-azide-free-ab219593--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66943.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)
Immunohistochemical staining of paraffin embedded mouse liver with purified Anti-STAT3 (phospho S727) antibody [E121-31] ab32143 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT3 (phospho S727) antibody [E121-31] ab32143).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593), expandable thumbnail](https://content.abcam.com/products/images/stat3-phospho-s727-antibody-e121-31-bsa-and-azide-free-ab219593--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32899.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)
Immunohistochemical staining of paraffin embedded human astrocytoma with purified Anti-STAT3 (phospho S727) antibody [E121-31] ab32143 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT3 (phospho S727) antibody [E121-31] ab32143).
![Immunocytochemistry/ Immunofluorescence - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593), expandable thumbnail](https://content.abcam.com/products/images/stat3-phospho-s727-antibody-e121-31-bsa-and-azide-free-ab219593--immunocytochemistry-immunofluorescence-img118166.jpg "Immunocytochemistry/ Immunofluorescence - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)")
Immunocytochemistry/ Immunofluorescence - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)
Purified Anti-STAT3 (phospho S727) antibody [E121-31] ab32143 staining STAT3 (phospho S727) in A431 cells by Immunocytochemistry/ Immunofluorescence. 4% PFA-fixed, 0.1% Triton X-100 permeabilized A431 (Human epidermoid carcinoma) cells labelled with Anti-STAT3 (phospho S727) antibody [E121-31] ab32143 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on A431 cell line. The red staining is Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (mouse mAb), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT3 (phospho S727) antibody [E121-31] ab32143).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593), expandable thumbnail](https://content.abcam.com/products/images/stat3-phospho-s727-antibody-e121-31-bsa-and-azide-free-ab219593--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117970.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)
IHC-P analysis of brain astrocytoma using unpurified Anti-STAT3 (phospho S727) antibody [E121-31] ab32143 at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT3 (phospho S727) antibody [E121-31] ab32143).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![ChIC/CUT&RUN sequencing - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593), expandable thumbnail](https://content.abcam.com/products/images/stat3-phospho-s727-antibody-e121-31-bsa-and-azide-free-ab219593--chic-cut-run-sequencing-img419264.jpg "ChIC/CUT&RUN sequencing - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)")
ChIC/CUT&RUN sequencing - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT3 (phospho S727) antibody [E121-31] ab32143).
ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HepG2 cells (starved overnight and treated with 100ng/ml IL-6 for 30min) and 5 µg of Anti-STAT3 (phospho S727) antibody [E121-31] ab32143 [E121-31]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
Additional screenshots of mapped reads can be found in the Protocol booklet in the Support and downloads section. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
![Immunocytochemistry/ Immunofluorescence - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593), expandable thumbnail](https://content.abcam.com/products/images/stat3-phospho-s727-antibody-e121-31-bsa-and-azide-free-ab219593--immunocytochemistry-immunofluorescence-img118166.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593), expandable thumbnail](https://content.abcam.com/products/images/stat3-phospho-s727-antibody-e121-31-bsa-and-azide-free-ab219593--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117970.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)")
![ChIC/CUT&RUN sequencing - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593), expandable thumbnail](https://content.abcam.com/products/images/stat3-phospho-s727-antibody-e121-31-bsa-and-azide-free-ab219593--chic-cut-run-sequencing-img419264.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-STAT3 (phospho S727) antibody [E121-31] - BSA and Azide free (ab219593)")
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