Rabbit Recombinant Monoclonal STAT4 phospho Y693 antibody. Suitable for WB, Dot, Flow Cyt (Intra), IP and reacts with Human, Transfected cell lysate - Human, Synthetic peptide samples.
Images
![Western blot - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (AB313630), expandable thumbnail](https://content.abcam.com/products/images/stat4-phospho-y693-antibody-epr27081-49-ab313630--western-blot-img418388.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (AB313630)")
![Western blot - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (AB313630), expandable thumbnail](https://content.abcam.com/products/images/stat4-phospho-y693-antibody-epr27081-49-ab313630--western-blot-img418387.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (AB313630)")
![Immunoprecipitation - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (AB313630), expandable thumbnail](https://content.abcam.com/products/images/stat4-phospho-y693-antibody-epr27081-49-ab313630--immunoprecipitation-img418423.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (AB313630)")
![Dot Blot - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (AB313630), expandable thumbnail](https://content.abcam.com/products/images/stat4-phospho-y693-antibody-epr27081-49-ab313630--dot-blot-img418509.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (AB313630)")
![Flow Cytometry (Intracellular) - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (AB313630), expandable thumbnail](https://content.abcam.com/products/images/stat4-phospho-y693-antibody-epr27081-49-ab313630--flow-cytometry-intracellular-img418499.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (AB313630)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Dot | Flow Cyt (Intra) | IP | IHC-P | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell lysate - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Transfected cell lysate - Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide, Mouse, Rat | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Synthetic peptide, Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Synthetic peptide, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Human, Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell lysate - Human, Synthetic peptide, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Transcriptional regulator mainly expressed in hematopoietic cells that plays a critical role in cellular growth, differentiation and immune response (PubMed:10961885, PubMed:37256972, PubMed:8943379). Plays a key role in the differentiation of T-helper 1 cells and the production of interferon-gamma (PubMed:12213961, PubMed:35614130). Participates also in multiple neutrophil functions including chemotaxis and production of the neutrophil extracellular traps (By similarity). After IL12 binding to its receptor IL12RB2, STAT4 interacts with the intracellular domain of IL12RB2 and becomes tyrosine phosphorylated (PubMed:10415122, PubMed:7638186). Phosphorylated STAT4 then homodimerizes and migrates to the nucleus where it can recognize STAT target sequences present in IL12 responsive genes. Although IL12 appears to be the predominant activating signal, STAT4 can also be phosphorylated and activated in response to IFN-gamma stimulation via JAK1 and TYK2 and in response to different interleukins including IL23, IL2 and IL35 (PubMed:11114383, PubMed:34508746). Transcription activation of IFN-gamma gene is mediated by interaction with JUN that forms a complex that efficiently interacts with the AP-1-related sequence of the IFN-gamma promoter (By similarity). In response to IFN-alpha/beta signaling, acts as a transcriptional repressor and suppresses IL5 and IL13 mRNA expression during response to T-cell receptor (TCR) activation (PubMed:26990433).
Alternative names
Signal transducer and activator of transcription 4, STAT4
Recommended products
Rabbit Recombinant Monoclonal STAT4 phospho Y693 antibody. Suitable for WB, Dot, Flow Cyt (Intra), IP and reacts with Human, Transfected cell lysate - Human, Synthetic peptide samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR27081-49
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Signal Transducer and Activator of Transcription 4 (STAT4) is a critical member of the STAT protein family. It weighs approximately 85 kDa. STAT4 primarily functions as a transcription factor activated by phosphorylation through Janus kinases (JAKs) in response to cytokine signaling. This protein facilitates the translocation into the nucleus where it promotes the transcription of specific genes. STAT4 expression occurs mainly in T cells and natural killer (NK) cells indicating its role in the immune response.
- Biological function summary
STAT4 is integral to the differentiation of naïve T cells into T helper 1 (Th1) cells. These Th1 cells play a central role in orchestrating immune responses against intracellular pathogens. STAT4 activation requires two distinct steps involving JAK kinases and subsequent binding to DNA regulatory elements of target genes. STAT4 does not function as part of a large protein complex but rather operates as a dimer following phosphorylation.
- Pathways
STAT4 participates strongly in the JAK-STAT signaling pathway fundamental for immune cell maturation and response. The JAK-STAT pathway regulates several biological processes including proliferation and inflammation. STAT4 works closely with STAT1 in promoting Th1 differentiation while also interacting with other proteins like IL-12 to modulate immune activity. These interactions emphasize its influence in immune responses and could affect inflammatory pathways.
- Associated diseases and disorders
STAT4 exhibits associations with autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant STAT4 activity can lead to dysregulated immune responses making it a significant target in these disorders. In the context of rheumatoid arthritis altered STAT4 signaling enhances inflammation through its connection with other immune proteins like IL-23. Understanding STAT4's role in these diseases may offer avenues for therapeutic interventions and better management of immune-related conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (ab313630), expandable thumbnail](https://content.abcam.com/products/images/stat4-phospho-y693-antibody-epr27081-49-ab313630--western-blot-img418388.jpg "Western blot - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (ab313630)")
Western blot - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (ab313630)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The antibody detects Human STAT4 (phospho Y693).
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
In Western blot, anti-STAT4 antibody (Anti-STAT4 antibody [EPR25128-48] ab284408) staining at 1/1000 dilution.
In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (ab313630) at 1/1000 dilution
Lane 1: 293T cells transfected with a Human STAT4 expression vector containing a His-tag were treated with treated with 100 mM Calyculin A for 30 minutes whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a Human STAT4 (Y693F mutation) expression vector containing a His-tag were treated with 100 mM Calyculin A for 30 minutes whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 85 kDa
Exposure time: 180s
![Western blot - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (ab313630), expandable thumbnail](https://content.abcam.com/products/images/stat4-phospho-y693-antibody-epr27081-49-ab313630--western-blot-img418387.jpg "Western blot - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (ab313630)")
Western blot - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (ab313630)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
In Western blot, anti-STAT4 antibody (Anti-STAT4 antibody [EPR25128-48] ab284408) staining at 1/1000 dilution.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (ab313630) at 1/1000 dilution
Lane 1: Untreated No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag) whole cell lysate (untreated membrane) at 20 µg
Lane 2: No-GFP-CD16.NK-92 treated with /mL IL-12 for 5 hours whole cell lysate (untreated membrane) at 20 µg
Lane 3: Untreated No-GFP-CD16.NK-92 whole cell lysate (phosphatase treated membrane) at 20 µg
Lane 4: No-GFP-CD16.NK-92 treated with /mL IL-12 for 5 hours whole cell lysate (phosphatase treated membrane) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 85 kDa
Exposure time: 180s
![Immunoprecipitation - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (ab313630), expandable thumbnail](https://content.abcam.com/products/images/stat4-phospho-y693-antibody-epr27081-49-ab313630--immunoprecipitation-img418423.jpg "Immunoprecipitation - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (ab313630)")
Immunoprecipitation - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (ab313630)
STAT4 (phospho Y693) was immunoprecipitated from 0.35 mg No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag) treated with 50 ng/mL IL-12 for 5 hours whole cell lysate with ab313630 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313630 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag) treated with 50 ng/mL IL-12 for 5 hours whole cell lysate
Lane 2: ab313630 IP in No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag) treated with 50 ng/mL IL-12 for 5 hours whole cell lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab313630 in No-GFP-CD16.NK-92 treated with 50 ng/mL IL-12 for 5 hours whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 secondsAll lanes: Immunoprecipitation - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (ab313630) at 1/30 dilution
All lanes: No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag) treated with 50 ng/mL IL-12 for 5 hours whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Exposure time: 180s
![Dot Blot - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (ab313630), expandable thumbnail](https://content.abcam.com/products/images/stat4-phospho-y693-antibody-epr27081-49-ab313630--dot-blot-img418509.jpg "Dot Blot - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (ab313630)")
Dot Blot - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (ab313630)
Dot blot analysis of STAT4 (phospho Y693) using ab313630 at 1:1000 (0.476 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane 1: STAT4 (phospho Y693) peptide a
Lane 2: STAT4 (phospho Y693) peptide b
Lane 3: STAT4 non-phospho peptide c
Exposure time: 180 seconds
Blocking and diluting buffer and concentration: 5% NFDM/TBST![Flow Cytometry (Intracellular) - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (ab313630), expandable thumbnail](https://content.abcam.com/products/images/stat4-phospho-y693-antibody-epr27081-49-ab313630--flow-cytometry-intracellular-img418499.jpg "Flow Cytometry (Intracellular) - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (ab313630)")
Flow Cytometry (Intracellular) - Anti-STAT4 (phospho Y693) antibody [EPR27081-49] (ab313630)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag) treated with 50ng/ml IL-12 for 5h (Red) / Untreated No-GFP-CD16.NK-92 (Green) cells labelling STAT4 (phospho Y693) with ab313630 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
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