Mouse Monoclonal STAT5a antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Dog, African green monkey, Recombinant full length protein samples. Cited in 11 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human STAT5A.
Images
![Western blot - Anti-STAT5 antibody [9F7] (AB230670), expandable thumbnail](https://content.abcam.com/products/images/stat5-antibody-9f7-ab230670--western-blot-img3743.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STAT5 antibody [9F7] (AB230670)")
![Western blot - Anti-STAT5 antibody [9F7] (AB230670), expandable thumbnail](https://content.abcam.com/products/images/stat5-antibody-9f7-ab230670--western-blot-img33366.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STAT5 antibody [9F7] (AB230670)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [9F7] (AB230670), expandable thumbnail](https://content.abcam.com/products/images/stat5-antibody-9f7-ab230670--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88555.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [9F7] (AB230670)")
![Immunocytochemistry/ Immunofluorescence - Anti-STAT5 antibody [9F7] (AB230670), expandable thumbnail](https://content.abcam.com/products/images/stat5-antibody-9f7-ab230670--immunocytochemistry-immunofluorescence-img5353.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-STAT5 antibody [9F7] (AB230670)")
![Flow Cytometry (Intracellular) - Anti-STAT5 antibody [9F7] (AB230670), expandable thumbnail](https://content.abcam.com/products/images/stat5-antibody-9f7-ab230670--flow-cytometry-intracellular-img59057.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-STAT5 antibody [9F7] (AB230670)")
Publications
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- Immunity, inflammation and disease 11:e10712023Astragalus polysaccharide ameliorates insulin resistance in HepG2 cells through activating the STAT5/IGF-1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinxin Yue,Wei Hao,Min Wang,Yang FuPubMed 38018587
- Oncology reports 50:2023Targeting of STAT5 using the small molecule topotecan hydrochloride suppresses acute myeloid leukemia progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiahui Li,Bin Tang,Ying Miao,Guihong Li,Zhenliang SunPubMed 37830151
- Cell death & disease 14:5252023Methionine orchestrates the metabolism vulnerability in cisplatin resistant bladder cancer microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Yang,Yuxi Ou,Quan Zhou,Yingchun Liang,Weijian Li,Yiling Chen,Wensun Chen,Siqi Wu,Yifan Chen,Xiyu Dai,Xinan Chen,Tian Chen,Shengming Jin,Yufei Liu,Limin Zhang,Shenghua Liu,Yun Hu,Lujia Zou,Shanhua Mao,Haowen JiangPubMed 37582769
- International journal of biological sciences 19:3661-36772023A novel mouse model of heart failure with preserved ejection fraction after chronic kidney disease induced by retinol through JAK/STAT pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBowen Liu,Adilan Shalamu,Zhiqiang Pei,Liwei Liu,Zilun Wei,Yanan Qu,Shuai Song,Wei Luo,Zhen Dong,Xinyu Weng,Junbo GePubMed 37564202
- Frontiers in medicine 8:7967432022Shionone Attenuates Sepsis-Induced Acute Kidney Injury by Regulating Macrophage Polarization the ECM1/STAT5 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBiao Zhang,Yi Xue,Jin Zhao,Huojun Jiang,Jiaoli Zhu,Hao Yin,Yizhen Qiu,Aihao Hu,Lingqi Xu,Yi Song,Xin WangPubMed 35141243
- Bioengineered 13:3724-37382022Aging affects the biological activity of fibroblast growth factor (FGF) in gastric epithelial cell, which is partially rescued by uridine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaomei Zhang,Huifeng Zhang,Jingli Gong,Huan Yu,Di Wu,Junyu Hou,Minghui Li,Xin SunPubMed 35105283
- International journal of molecular sciences 22:2021Increased Expression of Thymic Stromal Lymphopoietin in Chronic Constriction Injury of Rat Nerve.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChieh-Hsin Wu,Chun-Ching Lu,Chao-Lan Huang,Ming-Kung Wu,Ying-Yi LuPubMed 34281158
- Experimental and therapeutic medicine 20:3343-33512020CCL26 regulates the proportion of CD4CD25FOXP3 Tregs and the production of inflammatory factors in peripheral blood mononuclear cells following acute ischemic stroke via the STAT5 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiqiang Dong et. al.PubMed 32855707
- Molecular medicine reports 21:2522-25322020CCL11 increases the proportion of CD4+CD25+Foxp3+ Treg cells and the production of IL‑2 and TGF‑β by CD4+ T cells via the STAT5 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRong Wang et. al.PubMed 32323817
- Molecular medicine reports 21:2267-22752020Rat bone mesenchymal stem cells exert antiproliferative effects on nicotine‑exposed T cells via iNOS production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyan Li et. al.PubMed 32186760
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human STAT5A. Database link P42229
Consider this alternative
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected | Expected |
| African green monkey | Expected | Tested | Expected | Expected |
| Dog | Expected | Tested | Expected | Expected |
| Recombinant full length protein | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species Dog | Dilution info - | Notes - |
Species African green monkey | Dilution info 10 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/2000 | Notes - |
Species Rat | Dilution info 1/500 - 1/2000 | Notes - |
Species Dog | Dilution info 1/500 - 1/2000 | Notes - |
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
Species African green monkey | Dilution info 1/500 - 1/2000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species Dog | Dilution info - | Notes - |
Species African green monkey | Dilution info 1/100 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog, African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
Alternative names
STAT5, STAT5A, Signal transducer and activator of transcription 5A
Recommended products
Mouse Monoclonal STAT5a antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Dog, African green monkey, Recombinant full length protein samples. Cited in 11 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human STAT5A.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human STAT5A. Database link P42229
- Clone number
- 9F7
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Signal Transducer and Activator of Transcription 5 commonly known as STAT5 is a member of the STAT protein family. It weighs approximately 94 kDa. STAT5 usually exists in two closely related forms STAT5a and STAT5b with both playing similar roles but having slightly different activity. This protein is widely expressed found in cells like lymphocytes epithelial cells and endothelial cells indicating its importance in various tissues.
- Biological function summary
STAT5 acts as a transcription factor that modulates gene expression by binding to specific DNA sequences. It often forms part of a larger complex when activated. This complex relocation to the nucleus triggers transcriptional activities essential for cell growth differentiation and survival. As it interacts with other proteins STAT5 plays an important role in cytokine signaling particularly in response to interleukins and growth hormones.
- Pathways
STAT5 is important in the JAK-STAT signaling pathway. This pathway is activated by cytokines and growth factors leading to STAT5 phosphorylation and its subsequent translocation into the nucleus. The interaction with Janus kinases (JAKs) facilitates this process. STAT5 also plays a significant role in the MAPK pathway linking it to various cellular responses and functions.
- Associated diseases and disorders
STAT5 has a strong association with oncological conditions like leukemia and lymphomas. Aberrant activation or dysregulation of STAT5 can lead to uncontrolled cell proliferation and survival contributing to cancer. Furthermore STAT5 is linked with autoimmune diseases with its malfunction affecting immune response and leading to disorders. In these conditions proteins like BCL-XL often interact with STAT5 influencing cell survival pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-STAT5 antibody [9F7] (ab230670), expandable thumbnail](https://content.abcam.com/products/images/stat5-antibody-9f7-ab230670--western-blot-img3743.jpg "Western blot - Anti-STAT5 antibody [9F7] (ab230670)")
Western blot - Anti-STAT5 antibody [9F7] (ab230670)
All lanes: Western blot - Anti-STAT5 antibody [9F7] (ab230670) at 1/500 dilution
Lane 1: Untransfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg
Lane 2: STAT5-transfected HEK-293T cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 91 kDa
![Western blot - Anti-STAT5 antibody [9F7] (ab230670), expandable thumbnail](https://content.abcam.com/products/images/stat5-antibody-9f7-ab230670--western-blot-img33366.jpg "Western blot - Anti-STAT5 antibody [9F7] (ab230670)")
Western blot - Anti-STAT5 antibody [9F7] (ab230670)
All lanes: Western blot - Anti-STAT5 antibody [9F7] (ab230670) at 1/500 dilution
Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) cell extract at 35 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg
Lane 3: SVT2 cell extract at 35 µg
Lane 4: A549 (human lung carcinoma cell line) cell extract at 35 µg
Lane 5: COS-7 (African green monkey kidney fibroblast-like cell line) cell extract at 35 µg
Lane 6: Jurkat (human T cell leukemia cell line from peripheral blood) cell extract at 35 µg
Lane 7: MDCK (canine kidney cell line) cell extract at 35 µg
Lane 8: PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg
Lane 9: MCF7 (human breast adenocarcinoma cell line) cell extract at 35 µg
Developed using the ECL technique.
Predicted band size: 91 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [9F7] (ab230670), expandable thumbnail](https://content.abcam.com/products/images/stat5-antibody-9f7-ab230670--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88555.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [9F7] (ab230670)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [9F7] (ab230670)
Formalin-fixed, paraffin-embedded human testis tissue stained for STAT5 with ab230670 at 10 μg/ml in immunohistochemical analysis.
![Immunocytochemistry/ Immunofluorescence - Anti-STAT5 antibody [9F7] (ab230670), expandable thumbnail](https://content.abcam.com/products/images/stat5-antibody-9f7-ab230670--immunocytochemistry-immunofluorescence-img5353.jpg "Immunocytochemistry/ Immunofluorescence - Anti-STAT5 antibody [9F7] (ab230670)")
Immunocytochemistry/ Immunofluorescence - Anti-STAT5 antibody [9F7] (ab230670)
COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with STAT5 and stained for STAT5 (green) using ab230670 at 1/100 dilution.
![Flow Cytometry (Intracellular) - Anti-STAT5 antibody [9F7] (ab230670), expandable thumbnail](https://content.abcam.com/products/images/stat5-antibody-9f7-ab230670--flow-cytometry-intracellular-img59057.jpg "Flow Cytometry (Intracellular) - Anti-STAT5 antibody [9F7] (ab230670)")
Flow Cytometry (Intracellular) - Anti-STAT5 antibody [9F7] (ab230670)
Intracellular flow cytometric analysis ofHeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling STAT% with ab230670 at 1/100 dilution (red) compared with a negative control (blue).
![Flow Cytometry (Intracellular) - Anti-STAT5 antibody [9F7] (ab230670), expandable thumbnail](https://content.abcam.com/products/images/stat5-antibody-9f7-ab230670--flow-cytometry-intracellular-img73255.jpg "Flow Cytometry (Intracellular) - Anti-STAT5 antibody [9F7] (ab230670)")
Flow Cytometry (Intracellular) - Anti-STAT5 antibody [9F7] (ab230670)
Intracellular flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling STAT% with ab230670 at 1/100 dilution (red) compared with a negative control (blue).
![Flow Cytometry (Intracellular) - Anti-STAT5 antibody [9F7] (ab230670), expandable thumbnail](https://content.abcam.com/products/images/stat5-antibody-9f7-ab230670--flow-cytometry-intracellular-img73255.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-STAT5 antibody [9F7] (ab230670)")
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