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AB230670

Anti-STAT5 antibody [9F7]

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(16 Publications)

Mouse Monoclonal STAT5a antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Dog, African green monkey, Recombinant full length protein samples. Cited in 16 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human STAT5A.

View Alternative Names

STAT5, STAT5A, Signal transducer and activator of transcription 5A

6 Images
Flow Cytometry (Intracellular) - Anti-STAT5 antibody [9F7] (AB230670)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-STAT5 antibody [9F7] (AB230670)

Intracellular flow cytometric analysis ofHeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling STAT% with ab230670 at 1/100 dilution (red) compared with a negative control (blue).

Flow Cytometry (Intracellular) - Anti-STAT5 antibody [9F7] (AB230670)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-STAT5 antibody [9F7] (AB230670)

Intracellular flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling STAT% with ab230670 at 1/100 dilution (red) compared with a negative control (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [9F7] (AB230670)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [9F7] (AB230670)

Formalin-fixed, paraffin-embedded human testis tissue stained for STAT5 with ab230670 at 10 μg/ml in immunohistochemical analysis.

Western blot - Anti-STAT5 antibody [9F7] (AB230670)
  • WB

Supplier Data

Western blot - Anti-STAT5 antibody [9F7] (AB230670)

All lanes:

Western blot - Anti-STAT5 antibody [9F7] (ab230670) at 1/500 dilution

Lane 1:

Untransfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Lane 2:

STAT5-transfected HEK-293T cell lysate at 5 µg

Predicted band size: 91 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-STAT5 antibody [9F7] (AB230670)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STAT5 antibody [9F7] (AB230670)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with STAT5 and stained for STAT5 (green) using ab230670 at 1/100 dilution.

Western blot - Anti-STAT5 antibody [9F7] (AB230670)
  • WB

Supplier Data

Western blot - Anti-STAT5 antibody [9F7] (AB230670)

All lanes:

Western blot - Anti-STAT5 antibody [9F7] (ab230670) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell extract at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

Lane 3:

SVT2 cell extract at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) cell extract at 35 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extract at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

Lane 7:

MDCK (canine kidney cell line) cell extract at 35 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) cell extract at 35 µg

Predicted band size: 91 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9F7

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Dog, Human, African green monkey

Applications

WB, IHC-P, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

Recombinant Full Length Protein corresponding to Human STAT5A.

P42229

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant full length protein": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Signal Transducer and Activator of Transcription 5 commonly known as STAT5 is a member of the STAT protein family. It weighs approximately 94 kDa. STAT5 usually exists in two closely related forms STAT5a and STAT5b with both playing similar roles but having slightly different activity. This protein is widely expressed found in cells like lymphocytes epithelial cells and endothelial cells indicating its importance in various tissues.
Biological function summary

STAT5 acts as a transcription factor that modulates gene expression by binding to specific DNA sequences. It often forms part of a larger complex when activated. This complex relocation to the nucleus triggers transcriptional activities essential for cell growth differentiation and survival. As it interacts with other proteins STAT5 plays an important role in cytokine signaling particularly in response to interleukins and growth hormones.

Pathways

STAT5 is important in the JAK-STAT signaling pathway. This pathway is activated by cytokines and growth factors leading to STAT5 phosphorylation and its subsequent translocation into the nucleus. The interaction with Janus kinases (JAKs) facilitates this process. STAT5 also plays a significant role in the MAPK pathway linking it to various cellular responses and functions.

STAT5 has a strong association with oncological conditions like leukemia and lymphomas. Aberrant activation or dysregulation of STAT5 can lead to uncontrolled cell proliferation and survival contributing to cancer. Furthermore STAT5 is linked with autoimmune diseases with its malfunction affecting immune response and leading to disorders. In these conditions proteins like BCL-XL often interact with STAT5 influencing cell survival pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Carries out a dual function : signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
See full target information STAT5A
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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1503971 PubMed40206088

2025

Neuroprotective effects of arctigenin on cerebral ischemia-reperfusion injury in rats via the EPO/EPOR-JAK2-STAT5 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Xu,Yuting Chen,Lingling Zhang,Wei Lu,Xu Chen,Ting Wang,Wenjie Wang

Respiratory research 25:428 PubMed39633343

2024

T follicular helper cell is essential for M2 macrophage polarization and pulmonary vascular remodeling in hypoxia-induced pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Li,Pingping Liu,Hao Zhu,Huan Yang,Jun Zha,Huiling Yao,Shaoze Zhang,Jin Huang,Guang Li,Gang Jiang,Yongliang Jiang,Aiguo Dai

Scientific reports 14:29278 PubMed39587118

2024

EIF4A3-induced hsa_circ_0127071 promotes human glomerular mesangial cells senescence via JAK2/STAT5 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Chen,Xinwang Zhu,Da Sun,Li Yao,Shuang Yang,Lining Wang

Immunity, inflammation and disease 11:e1071 PubMed38018587

2023

Astragalus polysaccharide ameliorates insulin resistance in HepG2 cells through activating the STAT5/IGF-1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxin Yue,Wei Hao,Min Wang,Yang Fu

Oncology reports 50: PubMed37830151

2023

Targeting of STAT5 using the small molecule topotecan hydrochloride suppresses acute myeloid leukemia progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahui Li,Bin Tang,Ying Miao,Guihong Li,Zhenliang Sun

Cell death & disease 14:525 PubMed37582769

2023

Methionine orchestrates the metabolism vulnerability in cisplatin resistant bladder cancer microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Yang,Yuxi Ou,Quan Zhou,Yingchun Liang,Weijian Li,Yiling Chen,Wensun Chen,Siqi Wu,Yifan Chen,Xiyu Dai,Xinan Chen,Tian Chen,Shengming Jin,Yufei Liu,Limin Zhang,Shenghua Liu,Yun Hu,Lujia Zou,Shanhua Mao,Haowen Jiang

International journal of biological sciences 19:3661-3677 PubMed37564202

2023

A novel mouse model of heart failure with preserved ejection fraction after chronic kidney disease induced by retinol through JAK/STAT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bowen Liu,Adilan Shalamu,Zhiqiang Pei,Liwei Liu,Zilun Wei,Yanan Qu,Shuai Song,Wei Luo,Zhen Dong,Xinyu Weng,Junbo Ge

Frontiers in oncology 12:1069875 PubMed36518312

2022

Hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta gene as a tumour suppressor in stomach adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Li,Jian-Bo Xiong,Zhi-Gang Jie,Hui Xiong

Frontiers in medicine 8:796743 PubMed35141243

2022

Shionone Attenuates Sepsis-Induced Acute Kidney Injury by Regulating Macrophage Polarization the ECM1/STAT5 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Biao Zhang,Yi Xue,Jin Zhao,Huojun Jiang,Jiaoli Zhu,Hao Yin,Yizhen Qiu,Aihao Hu,Lingqi Xu,Yi Song,Xin Wang

Bioengineered 13:3724-3738 PubMed35105283

2022

Aging affects the biological activity of fibroblast growth factor (FGF) in gastric epithelial cell, which is partially rescued by uridine.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomei Zhang,Huifeng Zhang,Jingli Gong,Huan Yu,Di Wu,Junyu Hou,Minghui Li,Xin Sun
View all publications
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