AB227687

Anti-STAT5 antibody [SP283] - C-terminal

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal STAT5a antibody. C-terminal. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications.

View Alternative Names

STAT5, STAT5A, Signal transducer and activator of transcription 5A

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Formalin-fixed paraffin-embedded human bladder transitional cell carcinoma tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Formalin-fixed paraffin-embedded human bladder transitional cell carcinoma tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Formalin-fixed paraffin-embedded human skin squamous cell carcinoma tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Formalin-fixed paraffin-embedded human colon adenocarcinoma tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Formalin-fixed paraffin-embedded human cervical squamous cell carcinoma tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Formalin-fixed paraffin-embedded human endometrial adenocarcinoma tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Formalin-fixed paraffin-embedded human bladder tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Formalin-fixed paraffin-embedded human ovarian adenocarcinoma tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Formalin-fixed paraffin-embedded human B-Cell lymphoma tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Formalin-fixed paraffin-embedded human colon adenocarcinoma tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.

Carrier free

Anti-STAT5 antibody [SP283] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP283

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Boil tissue section in EDTA buffer (Cat# DEBX-050), pH 8.0 for 10 minfollowed by cooling at room temperature for 20 min.</p><p>Incubate with ab227687 for 10 minutes at room temperature.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Signal Transducer and Activator of Transcription 5 commonly known as STAT5 is a member of the STAT protein family. It weighs approximately 94 kDa. STAT5 usually exists in two closely related forms STAT5a and STAT5b with both playing similar roles but having slightly different activity. This protein is widely expressed found in cells like lymphocytes epithelial cells and endothelial cells indicating its importance in various tissues.

Biological function summary

STAT5 acts as a transcription factor that modulates gene expression by binding to specific DNA sequences. It often forms part of a larger complex when activated. This complex relocation to the nucleus triggers transcriptional activities essential for cell growth differentiation and survival. As it interacts with other proteins STAT5 plays an important role in cytokine signaling particularly in response to interleukins and growth hormones.

Pathways

STAT5 is important in the JAK-STAT signaling pathway. This pathway is activated by cytokines and growth factors leading to STAT5 phosphorylation and its subsequent translocation into the nucleus. The interaction with Janus kinases (JAKs) facilitates this process. STAT5 also plays a significant role in the MAPK pathway linking it to various cellular responses and functions.

STAT5 has a strong association with oncological conditions like leukemia and lymphomas. Aberrant activation or dysregulation of STAT5 can lead to uncontrolled cell proliferation and survival contributing to cancer. Furthermore STAT5 is linked with autoimmune diseases with its malfunction affecting immune response and leading to disorders. In these conditions proteins like BCL-XL often interact with STAT5 influencing cell survival pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Carries out a dual function : signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.

See full target information STAT5A

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:441 PubMed38909034

2024

Overexpression of TBX3 suppresses tumorigenesis in experimental and human cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Deng,Xinjun Lu,Xue Wang,Binyong Liang,Hongwei Xu,Doris Yang,Guofei Cui,Andrew Yonemura,Honor Paine,Yi Zhou,Yi Zhang,Maria Maddalena Simile,Francesco Urigo,Matthias Evert,Diego F Calvisi,Benjamin L Green,Xin Chen

Frontiers in immunology 11:1415 PubMed32733471

2020

Csf2 Attenuated Sepsis-Induced Acute Kidney Injury by Promoting Alternative Macrophage Transition.

Applications

Unspecified application

Species

Unspecified reactive species

Yiming Li,Pan Zhai,Yawen Zheng,Jing Zhang,John A Kellum,Zhiyong Peng

Cell death & disease 11:508 PubMed32641713

2020

Oxidized ATM promotes breast cancer stem cell enrichment through energy metabolism reprogram-mediated acetyl-CoA accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Yang,Meixi Peng,Yixuan Hou,Yilu Qin,Xueying Wan,Pengpeng Zhu,Shuiqing Liu,Liping Yang,Huan Zeng,Ting Jin,Yuxiang Qiu,Qiao Li,Manran Liu

Cancers 11: PubMed31783691

2019

Enhanced Hsa-miR-181d/p-STAT3 and Hsa-miR-181d/p-STAT5A Ratios Mediate the Anticancer Effect of Garcinol in -Addicted Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Heng-Wei Liu,Peter Mingjui Lee,Oluwaseun Adebayo Bamodu,Yu-Kai Su,Iat-Hang Fong,Chi-Tai Yeh,Ming-Hsien Chien,I-Hung Kan,Chien-Min Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-STAT5 antibody [SP283] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise