JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB98338

Anti-STAT5 (phospho Y694) antibody

Be the first to review this product! Submit a review

|

(5 Publications)

Rabbit Polyclonal STAT5a phospho Y694 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

STAT5, STAT5A, Signal transducer and activator of transcription 5A

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-STAT5 (phospho Y694) antibody (AB98338)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-STAT5 (phospho Y694) antibody (AB98338)

ICC/IF image of ab98338 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab98338 at 5μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Western blot - Anti-STAT5 (phospho Y694) antibody (AB98338)
  • WB

Unknown

Western blot - Anti-STAT5 (phospho Y694) antibody (AB98338)

All lanes:

Western blot - Anti-STAT5 (phospho Y694) antibody (ab98338) at 1 µg/mL

Lane 1:

EGF-Stimulated A431 Whole Cell Lysate at 10 µg

Lane 2:

EGF-Stimulated A431 Whole Cell Lysate at 10 µg with immunizing peptide

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 91 kDa

Observed band size: 185 kDa,91 kDa

true

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB126832

Anti-STAT5 antibody

Western blot - Anti-STAT5 antibody (AB126832)

  • 0
  • 0
Primary Antibodies

AB32364

Anti-STAT5 (phospho Y694) antibody [E208]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (AB32364)

  • 4
  • 11
Primary Antibodies

AB219607

Anti-STAT5 (phospho Y694) antibody [E208] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] - BSA and Azide free (AB219607)

  • 0
  • 0
Primary Antibodies

AB230670

Anti-STAT5 antibody [9F7]

Flow Cytometry (Intracellular) - Anti-STAT5 antibody [9F7] (AB230670)

  • 0
  • 0
Primary Antibodies

AB227687

Anti-STAT5 antibody [SP283] - C-terminal

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 antibody [SP283] - C-terminal (AB227687)

  • 0
  • 0

Complementary Products

Assay Kits

AB126429

Human STAT5 (Tyr694) In-Cell ELISA Kit

In-Cell ELISA - Human STAT5 (Tyr694) In-Cell ELISA Kit (AB126429)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32520

Anti-STAT6 antibody [YE361]

Recombinant|Advanced Validation|RabMAb|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (AB32520)

  • 5
  • 1
Primary Antibodies

AB178941

Anti-STAT5b antibody [EPR16671]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-STAT5b antibody [EPR16671] (AB178941)

  • 5
  • 4
Primary Antibodies

AB68153

Anti-STAT3 antibody [EPR787Y]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Western blot - Anti-STAT3 antibody [EPR787Y] (AB68153)

  • 4
  • 10
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Signal Transducer and Activator of Transcription 5 commonly known as STAT5 is a member of the STAT protein family. It weighs approximately 94 kDa. STAT5 usually exists in two closely related forms STAT5a and STAT5b with both playing similar roles but having slightly different activity. This protein is widely expressed found in cells like lymphocytes epithelial cells and endothelial cells indicating its importance in various tissues.
Biological function summary

STAT5 acts as a transcription factor that modulates gene expression by binding to specific DNA sequences. It often forms part of a larger complex when activated. This complex relocation to the nucleus triggers transcriptional activities essential for cell growth differentiation and survival. As it interacts with other proteins STAT5 plays an important role in cytokine signaling particularly in response to interleukins and growth hormones.

Pathways

STAT5 is important in the JAK-STAT signaling pathway. This pathway is activated by cytokines and growth factors leading to STAT5 phosphorylation and its subsequent translocation into the nucleus. The interaction with Janus kinases (JAKs) facilitates this process. STAT5 also plays a significant role in the MAPK pathway linking it to various cellular responses and functions.

STAT5 has a strong association with oncological conditions like leukemia and lymphomas. Aberrant activation or dysregulation of STAT5 can lead to uncontrolled cell proliferation and survival contributing to cancer. Furthermore STAT5 is linked with autoimmune diseases with its malfunction affecting immune response and leading to disorders. In these conditions proteins like BCL-XL often interact with STAT5 influencing cell survival pathways.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Carries out a dual function : signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
See full target information STAT5A phospho Y694
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Veterinary sciences 12: PubMed40284805

2025

Effects of Ladder-Climbing Exercise on Mammary Cancer: Data from a Chemically Induced Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Silva,Tiago Azevedo,Inês Aires,Catarina Medeiros,Maria J Neuparth,Fernanda Seixas,Rita Ferreira,Ana I Faustino-Rocha,Paula A Oliveira,José Alberto Duarte

Biochemistry and biophysics reports 39:101773 PubMed39044768

2024

Smoking-induced suppression of β-casein in milk is associated with an increase in miR-210-5p expression in mammary epithelia.

Applications

Unspecified application

Species

Unspecified reactive species

Takeshi Chiba,Akira Takaguri,Toshiyasu Mikuma,Toshimi Kimura,Tomoji Maeda

Frontiers in medicine 8:796743 PubMed35141243

2022

Shionone Attenuates Sepsis-Induced Acute Kidney Injury by Regulating Macrophage Polarization the ECM1/STAT5 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Biao Zhang,Yi Xue,Jin Zhao,Huojun Jiang,Jiaoli Zhu,Hao Yin,Yizhen Qiu,Aihao Hu,Lingqi Xu,Yi Song,Xin Wang

General and comparative endocrinology 296:113545 PubMed32622934

2020

Acute sleep deprivation leads to growth hormone (GH) resistance in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xintong Lyu,Guohua Wang,Zhuang Pi,Lan Wu

Journal of experimental & clinical cancer research 38:465 PubMed31718693

2019

Long non-coding RNA RP11-468E2.5 curtails colorectal cancer cell proliferation and stimulates apoptosis via the JAK/STAT signaling pathway by targeting STAT5 and STAT6.

Applications

Unspecified application

Species

Unspecified reactive species

Li Jiang,Xu-Hai Zhao,Yin-Ling Mao,Jun-Feng Wang,Hui-Jun Zheng,Qing-Shan You
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com