Anti-STAT5 (phospho Y694) antibody [E208] (ab32364) is a rabbit monoclonal antibody that is used to detect STAT5 in Western Blot, IHC-P, ELISA. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 70 publications
-Over 70 publications
Images
![ELISA - Anti-STAT5 (phospho Y694) antibody [E208] (AB32364), expandable thumbnail](https://content.abcam.com/products/images/stat5-phospho-y694-antibody-e208-ab32364--elisa-img76989.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-STAT5 (phospho Y694) antibody [E208] (AB32364)")
![Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (AB32364), expandable thumbnail](https://content.abcam.com/products/images/stat5-phospho-y694-antibody-e208-ab32364--western-blot-img12315.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (AB32364)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (AB32364), expandable thumbnail](https://content.abcam.com/products/images/stat5-phospho-y694-antibody-e208-ab32364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127076.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (AB32364)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (AB32364), expandable thumbnail](https://content.abcam.com/products/images/stat5-phospho-y694-antibody-e208-ab32364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99242.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (AB32364)")
Publications
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- Immunity, inflammation and disease 11:e10712023Astragalus polysaccharide ameliorates insulin resistance in HepG2 cells through activating the STAT5/IGF-1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinxin Yue,Wei Hao,Min Wang,Yang FuPubMed 38018587
- Pathogens (Basel, Switzerland) 12:2023-Knockout Induces Abnormal Metabolic Phenotype via the Activation of Hepatic STAT5/PDK4 Signaling in Diabetic Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYilang Li,Jiaxuan Cai,Yinan Liu,Conglin Li,Xiaoqing Chen,Wing-Leung Wong,Wenyue Jiang,Yuan Qin,Guiping Zhang,Ning Hou,Wenchang YuanPubMed 38003764
- Oncology reports 50:2023Targeting of STAT5 using the small molecule topotecan hydrochloride suppresses acute myeloid leukemia progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiahui Li,Bin Tang,Ying Miao,Guihong Li,Zhenliang SunPubMed 37830151
- Cell death & disease 14:5252023Methionine orchestrates the metabolism vulnerability in cisplatin resistant bladder cancer microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Yang,Yuxi Ou,Quan Zhou,Yingchun Liang,Weijian Li,Yiling Chen,Wensun Chen,Siqi Wu,Yifan Chen,Xiyu Dai,Xinan Chen,Tian Chen,Shengming Jin,Yufei Liu,Limin Zhang,Shenghua Liu,Yun Hu,Lujia Zou,Shanhua Mao,Haowen JiangPubMed 37582769
- International journal of biological sciences 19:3661-36772023A novel mouse model of heart failure with preserved ejection fraction after chronic kidney disease induced by retinol through JAK/STAT pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBowen Liu,Adilan Shalamu,Zhiqiang Pei,Liwei Liu,Zilun Wei,Yanan Qu,Shuai Song,Wei Luo,Zhen Dong,Xinyu Weng,Junbo GePubMed 37564202
- EMBO reports 24:e566892023STAT5b is a key effector of NRG-1/ERBB4-mediated myocardial growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatri Vaparanta,Anne Jokilammi,Ilkka Paatero,Johannes A Merilahti,Juho Heliste,Karthik Amudhala Hemanthakumar,Riikka Kivelä,Kari Alitalo,Pekka Taimen,Klaus EleniusPubMed 37009825
- Cell transplantation 32:96368972211494442023CD27-Expressing Xenoantigen-Expanded Human Regulatory T Cells Are Efficient in Suppressing Xenogeneic Immune Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Cao,Xiaoqian Ma,Juan Zhang,Min Yang,Zhenhu He,Cejun Yang,Sang Li,Pengfei Rong,Wei WangPubMed 36644879
- Frontiers in oncology 12:10179472022Disrupting PTPRJ transmembrane-mediated oligomerization counteracts oncogenic receptor tyrosine kinase FLT3 ITD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie Schwarz,Sophie Rizzo,Walter Espinoza Paz,Anne Kresinsky,Damien Thévenin,Jörg P MüllerPubMed 36452504
- Haematologica 107:2589-26002022Interplay between hypertriglyceridemia and acute promyelocytic leukemia mediated by the cooperation of peroxisome proliferator-activated receptor-α with the PML/RAR α fusion protein on super-enhancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShishuang Wu,Shufen Li,Peng Jin,Yi Zhang,Li Chen,Wen Jin,Junmin Li,Kankan WangPubMed 35546300
- Journal of medicinal chemistry 65:8380-84002022Insights on JAK2 Modulation by Potent, Selective, and Cell-Permeable Pseudokinase-Domain Ligands.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria-Elena Liosi,Joseph A Ippolito,Sean P Henry,Stefan G Krimmer,Ana S Newton,Kara J Cutrona,Rene A Olivarez,Jyotidarsini Mohanty,Joseph Schlessinger,William L JorgensenPubMed 35653642
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | ELISA | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Expected | Tested | Not recommended | Tested |
| Mouse | Not recommended | Predicted | Predicted | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Synthetic peptide | Dilution info - | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Synthetic peptide | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes Unsuitable for mouse. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Unsuitable for mouse. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
Alternative names
STAT5, STAT5A, Signal transducer and activator of transcription 5A
Recommended products
Anti-STAT5 (phospho Y694) antibody [E208] (ab32364) is a rabbit monoclonal antibody that is used to detect STAT5 in Western Blot, IHC-P, ELISA. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 70 publications
-Over 70 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- E208
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-STAT5 (phospho Y694) antibody [E208] (ab32364) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), ELISA in Human samples.
What is the molecular weight of STAT5?
Anti-STAT5 (phospho Y694) [E208] (ab32364) specifically detects a band for STAT5 (UniProt: P42229) at a molecular weight of 90kDa.
Trusted by the scientific community
Anti-STAT5 (phospho Y694) [E208] (ab32364) was first used in a scientific publication in 2006 and has been cited over 70 times in peer-reviewed journals.
Reviewed by scientists
Anti-STAT5 (phospho Y694) [E208] (ab32364) has over 10 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [E208] also available for your convenience:
ab32364, Carrier free - Anti-STAT5 (phospho Y694) antibody [E208] - BSA and Azide free ab219607, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-STAT5 (phospho Y694) antibody [E208] ab310959, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-STAT5 (phospho Y694) antibody [E208] ab311076, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-STAT5 (phospho Y694) antibody [E208] ab311665, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-STAT5 (phospho Y694) antibody [E208] ab312939, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-STAT5 (phospho Y694) antibody [E208] ab313150
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Signal Transducer and Activator of Transcription 5 commonly known as STAT5 is a member of the STAT protein family. It weighs approximately 94 kDa. STAT5 usually exists in two closely related forms STAT5a and STAT5b with both playing similar roles but having slightly different activity. This protein is widely expressed found in cells like lymphocytes epithelial cells and endothelial cells indicating its importance in various tissues.
- Biological function summary
STAT5 acts as a transcription factor that modulates gene expression by binding to specific DNA sequences. It often forms part of a larger complex when activated. This complex relocation to the nucleus triggers transcriptional activities essential for cell growth differentiation and survival. As it interacts with other proteins STAT5 plays an important role in cytokine signaling particularly in response to interleukins and growth hormones.
- Pathways
STAT5 is important in the JAK-STAT signaling pathway. This pathway is activated by cytokines and growth factors leading to STAT5 phosphorylation and its subsequent translocation into the nucleus. The interaction with Janus kinases (JAKs) facilitates this process. STAT5 also plays a significant role in the MAPK pathway linking it to various cellular responses and functions.
- Associated diseases and disorders
STAT5 has a strong association with oncological conditions like leukemia and lymphomas. Aberrant activation or dysregulation of STAT5 can lead to uncontrolled cell proliferation and survival contributing to cancer. Furthermore STAT5 is linked with autoimmune diseases with its malfunction affecting immune response and leading to disorders. In these conditions proteins like BCL-XL often interact with STAT5 influencing cell survival pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![ELISA - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364), expandable thumbnail](https://content.abcam.com/products/images/stat5-phospho-y694-antibody-e208-ab32364--elisa-img76989.jpg "ELISA - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)")
ELISA - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
Serially diluted ab32364 was bound to immobilised phospho peptide (P) - or control peptides ((C), 1 microgram x mL-1). The antibody was detected by HRP-labelled goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080; diluted 1: 50,000) and signal was developed with TMB substrate
![Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364), expandable thumbnail](https://content.abcam.com/products/images/stat5-phospho-y694-antibody-e208-ab32364--western-blot-img12315.jpg "Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)")
Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364) at 1/1000 dilution
Lane 1: A431 cell lysate, untreated at 10 µg
Lane 2: A431 cell lysate, treated with Epidermal growth factor at 10 µg
SecondaryAll lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 91 kDa
Observed band size: 90 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364), expandable thumbnail](https://content.abcam.com/products/images/stat5-phospho-y694-antibody-e208-ab32364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127076.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma using unpurified ab32364 at a 1/50 dilution.
![Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364), expandable thumbnail](https://content.abcam.com/products/images/stat5-phospho-y694-antibody-e208-ab32364--western-blot-img71097.gif "Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)")
Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
All lanes: Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364) at 1/1000 dilution
Lane 1: A431 cell lysate, untreated
Lane 2: A431 cell lysate, treated with EGF
Lane 3: A431 cell lysate, treated with phosphatase
Predicted band size: 91 kDa
Observed band size: 90 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364), expandable thumbnail](https://content.abcam.com/products/images/stat5-phospho-y694-antibody-e208-ab32364--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99242.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human infiltrating duct carcinoma of breast tissue labelling STAT5 (phospho Y694) with purified ab32364 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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