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Rabbit Recombinant Monoclonal STAT5a antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 38 publications.


Images

Immunoprecipitation - Anti-STAT5a antibody [E289] (AB32043), expandable thumbnail
  • Western blot - Anti-STAT5a antibody [E289] (AB32043), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-STAT5a antibody [E289] (AB32043), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT5a antibody [E289] (AB32043), expandable thumbnail
  • Western blot - Anti-STAT5a antibody [E289] (AB32043), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Expected
Rat
Expected
Expected
Expected
Expected
Expected

Tested
Tested

Species

Human

Dilution info

1/20

Notes

For unpurified use at 1/80.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

1/20

Notes

For unpurified use at 1/80.

Species

Rat

Dilution info

1/20

Notes

For unpurified use at 1/80.

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Rat

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/500

Notes

-

Expected
Expected

Species

Mouse

Dilution info

1/100 - 1/500

Notes

-

Species

Rat

Dilution info

1/100 - 1/500

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.For unpurified use at 1/10.

Expected
Expected

Species

Mouse

Dilution info

1/100

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.For unpurified use at 1/10.

Species

Rat

Dilution info

1/100

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.For unpurified use at 1/10.

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

1/1000

Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.

Alternative names

Signal transducer and activator of transcription 5A, STAT5, STAT5A

Recommended products

Rabbit Recombinant Monoclonal STAT5a antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 38 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

E289

Purification technique

Affinity purification Protein A

Specificity

The antibody recognises Stat5a. It does not cross-react with other Stat family members.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

STAT5a also known as Signal Transducer and Activator of Transcription 5A acts as a transcription factor in the cell. STAT5a weighs approximately 90 kDa and expresses in various tissues including the liver muscle and hematopoietic cells. It plays a central role in mediating cellular responses to cytokines and growth factors particularly those in the interleukin-2 (IL-2) family and growth hormone (GH).

Biological function summary

Many systems use STAT5a to regulate cell proliferation differentiation and survival. It often forms a complex with other proteins like STAT5b which is a closely related counterpart and both contribute to similar cellular processes. STAT5a can dimerize upon activation allowing it to move to the cell nucleus to influence gene expression directly.

Pathways

Researchers place STAT5a prominently in the JAK-STAT signaling pathway. This pathway is essential for transmitting information received from extracellular chemical signals to the cell nucleus resulting in the activation of genes controlling cellular functions. STAT5a works together with Janus kinases (JAKs) to mediate various biological responses including hormone signaling and immune function. It also interacts with proteins like IL-2 receptor subunits which are involved in many cytokine-driven processes.

Associated diseases and disorders

Disturbances in STAT5a activity link specifically with conditions like leukemia and breast cancer. Overactivation or mutation can lead to uncontrolled cell growth and survival contributing to these diseases. In leukemia STAT5a frequently partners with other proteins like BCR-ABL which is known for its role in chronic myeloid leukemia influencing oncogenic pathways. In breast cancer alterations in STAT5a signaling may interact with estrogen and progesterone receptors affecting tumor progression and response to therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Immunoprecipitation - Anti-STAT5a antibody [E289] (ab32043), expandable thumbnail

    Immunoprecipitation - Anti-STAT5a antibody [E289] (ab32043)

    ab32043 (purified) at 1:20 dilution (0.6ug) immunoprecipitating in TF-1 whole cell lysate. TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10ug
    Lane 2 (+): ab32043 & TF-1 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab32043 in TF-1 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-STAT5a antibody [E289] (ab32043)

    Predicted band size: 90 kDa

    Observed band size: 92 kDa

  • Western blot - Anti-STAT5a antibody [E289] (ab32043), expandable thumbnail

    Western blot - Anti-STAT5a antibody [E289] (ab32043)

    All lanes: Western blot - Anti-STAT5a antibody [E289] (ab32043) at 1/20000 dilution

    All lanes: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 90 kDa

    Observed band size: 92 kDa

  • Flow Cytometry (Intracellular) - Anti-STAT5a antibody [E289] (ab32043), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-STAT5a antibody [E289] (ab32043)

    Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling STAT5a with purified ab32043 at 1/100 dilution (1 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-STAT5a antibody [E289] (ab32043), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-STAT5a antibody [E289] (ab32043)

    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (human T cell leukemia T lymphocyte) cells labeling STAT5a with purified ab32043 at 1:100 (1.2 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-STAT5a antibody [E289] (ab32043), expandable thumbnail

    Western blot - Anti-STAT5a antibody [E289] (ab32043)

    All lanes: Western blot - Anti-STAT5a antibody [E289] (ab32043) at 1/1000 dilution

    Lane 1: Mouse brain lysates at 15 µg

    Lane 2: Rat brain lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 90 kDa

    Observed band size: 92 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a antibody [E289] (ab32043), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a antibody [E289] (ab32043)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling STAT5a with purified ab32043 at 1:1000 dilution (0.12 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

  • Immunocytochemistry/ Immunofluorescence - Anti-STAT5a antibody [E289] (ab32043), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-STAT5a antibody [E289] (ab32043)

    Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling STAT5a with unpurified ab32043 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Western blot - Anti-STAT5a antibody [E289] (ab32043), expandable thumbnail

    Western blot - Anti-STAT5a antibody [E289] (ab32043)

    All lanes: Western blot - Anti-STAT5a antibody [E289] (ab32043) at 1/1000 dilution

    All lanes: A431 cell lysate

    Predicted band size: 90 kDa

    Observed band size: 92 kDa

  • Flow Cytometry (Intracellular) - Anti-STAT5a antibody [E289] (ab32043), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-STAT5a antibody [E289] (ab32043)

    Overlay histogram showing Jurkat cells stained with unpurified ab32043 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32043, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Western blot - Anti-STAT5a antibody [E289] (ab32043), expandable thumbnail

    Western blot - Anti-STAT5a antibody [E289] (ab32043)

    All lanes: Western blot - Anti-STAT5a antibody [E289] (ab32043) at 1/1000 dilution

    All lanes: Western blot - Recombinant Human STAT5a protein (Recombinant Human STAT5a protein ab84627) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 90 kDa

    Exposure time: 20s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a antibody [E289] (ab32043), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a antibody [E289] (ab32043)

    Unpurified ab32043 at 1/250 dilution, staining human squamous lung carcinoma by Immunohistochemistry, paraffin-embedded tissue. Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a antibody [E289] (ab32043), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a antibody [E289] (ab32043)

    Tissue Microarrays stained for "Anti-STAT5a antibody [E289]" using "ab32043"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with ab32043 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

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