AB248206

Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal STAT5a phospho S726 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 1 publication.

View Alternative Names

STAT5, STAT5A, Signal transducer and activator of transcription 5A

3 Images

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Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (AB248206)

This data was developed using ab128896, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (<a href='/en-us/products/primary-antibodies/stat5a-phospho-s726-antibody-epr19142-ab128896'>ab128896</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysates: untreated at 10 µg

Lane 2:

SH-SY5Y cell lysates: treated with Okadaic acid and Calyculin A at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 90 kDa

Observed band size: 90 kDa

false

Unknown

Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (AB248206)

This data was developed using ab128896, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (<a href='/en-us/products/primary-antibodies/stat5a-phospho-s726-antibody-epr19142-ab128896'>ab128896</a>) at 1/5000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma) treated with Okadaic acid and Calyculin A whole cell lysates at 10 µg

Lane 2:

Untreated SH-SY5Y (human neuroblastoma) whole cell lysates at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 90 kDa

Observed band size: 90 kDa

false

Exposure time: 3min

Unknown

Dot Blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (AB248206)

This data was developed using ab128896, the same antibody clone in a different buffer formulation.

Primary antibody dilution : 1/1000

Secondary antibody : goat anti-rabbit IgG, (H+L), peroxidase conjugated

Secondary antibody dilution : 1/2500

Blocking & dilution buffer : 5% NFDM/TBST

Lane 1 sample : STAT5a (pS726) phospho peptide

Lane 2 sample : STAT5a non-phospho peptide

Exposure time : 10 seconds

Unconjugated

Anti-STAT5a (phospho S726) antibody [EPR1914(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1914(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FuncS" : {"fullname" : "Functional Studies", "shortname":"FuncS"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FuncS-species-checked": "notRecommended", "FuncS-species-dilution-info": "", "FuncS-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FuncS-species-checked": "notRecommended", "FuncS-species-dilution-info": "", "FuncS-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FuncS-species-checked": "notRecommended", "FuncS-species-dilution-info": "", "FuncS-species-notes": "" }, "Synthetic peptide": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FuncS-species-checked": "notRecommended", "FuncS-species-dilution-info": "", "FuncS-species-notes": "" } } }

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Product details

ab248206 is the carrier-free version of ab128896.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STAT5a also known as Signal Transducer and Activator of Transcription 5A acts as a transcription factor in the cell. STAT5a weighs approximately 90 kDa and expresses in various tissues including the liver muscle and hematopoietic cells. It plays a central role in mediating cellular responses to cytokines and growth factors particularly those in the interleukin-2 (IL-2) family and growth hormone (GH).

Biological function summary

Many systems use STAT5a to regulate cell proliferation differentiation and survival. It often forms a complex with other proteins like STAT5b which is a closely related counterpart and both contribute to similar cellular processes. STAT5a can dimerize upon activation allowing it to move to the cell nucleus to influence gene expression directly.

Pathways

Researchers place STAT5a prominently in the JAK-STAT signaling pathway. This pathway is essential for transmitting information received from extracellular chemical signals to the cell nucleus resulting in the activation of genes controlling cellular functions. STAT5a works together with Janus kinases (JAKs) to mediate various biological responses including hormone signaling and immune function. It also interacts with proteins like IL-2 receptor subunits which are involved in many cytokine-driven processes.

Disturbances in STAT5a activity link specifically with conditions like leukemia and breast cancer. Overactivation or mutation can lead to uncontrolled cell growth and survival contributing to these diseases. In leukemia STAT5a frequently partners with other proteins like BCR-ABL which is known for its role in chronic myeloid leukemia influencing oncogenic pathways. In breast cancer alterations in STAT5a signaling may interact with estrogen and progesterone receptors affecting tumor progression and response to therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Carries out a dual function : signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.

See full target information STAT5A phospho S726

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:1025 PubMed39164573

2024

CD58 defines regulatory macrophages within the tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Wu,Xiaoni Zhan,Meixi Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free

Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (AB248206)

Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (AB248206)

Dot Blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (AB248206)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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