Rabbit Recombinant Monoclonal STAT5a phospho S726 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples.
Images
![Dot Blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (AB248206), expandable thumbnail](https://content.abcam.com/products/images/stat5a-phospho-s726-antibody-epr19142-bsa-and-azide-free-ab248206--dot-blot-img21350.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (AB248206)")
![Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (AB248206), expandable thumbnail](https://content.abcam.com/products/images/stat5a-phospho-s726-antibody-epr19142-bsa-and-azide-free-ab248206--western-blot-img21397.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (AB248206)")
![Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (AB248206), expandable thumbnail](https://content.abcam.com/products/images/stat5a-phospho-s726-antibody-epr19142-bsa-and-azide-free-ab248206--western-blot-img129215.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (AB248206)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Dot | WB | IHC-P | FuncS | |
|---|---|---|---|---|---|
| Human | Not recommended | Expected | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Predicted | Predicted | Not recommended | Not recommended |
| Rat | Not recommended | Predicted | Predicted | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
FuncS
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
Alternative names
STAT5, STAT5A, Signal transducer and activator of transcription 5A
Recommended products
Rabbit Recombinant Monoclonal STAT5a phospho S726 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR1914(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248206 is the carrier-free version of Anti-STAT5a (phospho S726) antibody [EPR1914(2)] ab128896.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
STAT5a also known as Signal Transducer and Activator of Transcription 5A acts as a transcription factor in the cell. STAT5a weighs approximately 90 kDa and expresses in various tissues including the liver muscle and hematopoietic cells. It plays a central role in mediating cellular responses to cytokines and growth factors particularly those in the interleukin-2 (IL-2) family and growth hormone (GH).
- Biological function summary
Many systems use STAT5a to regulate cell proliferation differentiation and survival. It often forms a complex with other proteins like STAT5b which is a closely related counterpart and both contribute to similar cellular processes. STAT5a can dimerize upon activation allowing it to move to the cell nucleus to influence gene expression directly.
- Pathways
Researchers place STAT5a prominently in the JAK-STAT signaling pathway. This pathway is essential for transmitting information received from extracellular chemical signals to the cell nucleus resulting in the activation of genes controlling cellular functions. STAT5a works together with Janus kinases (JAKs) to mediate various biological responses including hormone signaling and immune function. It also interacts with proteins like IL-2 receptor subunits which are involved in many cytokine-driven processes.
- Associated diseases and disorders
Disturbances in STAT5a activity link specifically with conditions like leukemia and breast cancer. Overactivation or mutation can lead to uncontrolled cell growth and survival contributing to these diseases. In leukemia STAT5a frequently partners with other proteins like BCR-ABL which is known for its role in chronic myeloid leukemia influencing oncogenic pathways. In breast cancer alterations in STAT5a signaling may interact with estrogen and progesterone receptors affecting tumor progression and response to therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Dot Blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206), expandable thumbnail](https://content.abcam.com/products/images/stat5a-phospho-s726-antibody-epr19142-bsa-and-azide-free-ab248206--dot-blot-img21350.jpg "Dot Blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)")
Dot Blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)
This data was developed using Anti-STAT5a (phospho S726) antibody [EPR1914(2)] ab128896, the same antibody clone in a different buffer formulation.Primary antibody dilution: 1/1000 Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated Secondary antibody dilution: 1/2500 Blocking & dilution buffer: 5% NFDM/TBST Lane 1 sample: STAT5a (pS726) phospho peptide Lane 2 sample: STAT5a non-phospho peptide Exposure time: 10 seconds
![Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206), expandable thumbnail](https://content.abcam.com/products/images/stat5a-phospho-s726-antibody-epr19142-bsa-and-azide-free-ab248206--western-blot-img21397.jpg "Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)")
Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)
This data was developed using Anti-STAT5a (phospho S726) antibody [EPR1914(2)] ab128896, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (Anti-STAT5a (phospho S726) antibody [EPR1914(2)] ab128896) at 1/5000 dilution
Lane 1: SH-SY5Y (human neuroblastoma) treated with Okadaic acid and Calyculin A whole cell lysates at 10 µg
Lane 2: Untreated SH-SY5Y (human neuroblastoma) whole cell lysates at 10 µg
SecondaryAll lanes: Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa
Exposure time: 3min
![Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206), expandable thumbnail](https://content.abcam.com/products/images/stat5a-phospho-s726-antibody-epr19142-bsa-and-azide-free-ab248206--western-blot-img129215.jpg "Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)")
Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (ab248206)
This data was developed using Anti-STAT5a (phospho S726) antibody [EPR1914(2)] ab128896, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (Anti-STAT5a (phospho S726) antibody [EPR1914(2)] ab128896) at 1/1000 dilution
Lane 1: SH-SY5Y cell lysates: untreated at 10 µg
Lane 2: SH-SY5Y cell lysates: treated with Okadaic acid and Calyculin A at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa
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