Anti-STAT5a (phospho S780) antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a (phospho S780) antibody (AB30649)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ab30649. The picture on the right is blocked with the phospho peptide.

• WB

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Western blot - Anti-STAT5a (phospho S780) antibody (AB30649)

All lanes:

Western blot - Anti-STAT5a (phospho S780) antibody (ab30649)

Lane 1:

HeLa cells extract

Lane 2:

HeLa cells extract with synthesized non-phosphopeptide

Lane 3:

HeLa cells extract with synthesized phosphopeptide

Predicted band size: 90 kDa

Observed band size: 91 kDa

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• WB

Supplier Data

Western blot - Anti-STAT5a (phospho S780) antibody (AB30649)

Loading control : beta Actin.

All lanes:

Western blot - Anti-STAT5a (phospho S780) antibody (ab30649) at 1/1000 dilution

Lane 1:

Jurkat untreated lysate at 20 µg

Lane 2:

Jurkat treated with 125 ng/mL PMA for 30 minutes lysate at 20 µg

Lane 3:

293T untreated lysate at 20 µg

Lane 4:

293T treated with 125 ng/mL PMA for 30 minutes lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution

Predicted band size: 90 kDa

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• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-STAT5a (phospho S780) antibody (AB30649)

ICC/IF image of ab30649 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30649, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.