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AB106095

Anti-STAT5a (phospho Y694) antibody [6.F1]

4

(1 Review)

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(2 Publications)

Mouse Monoclonal STAT5a phospho Y694 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Stat5a phospho Y694.

View Alternative Names

STAT5, STAT5A, Signal transducer and activator of transcription 5A

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a (phospho Y694) antibody [6.F1] (AB106095)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a (phospho Y694) antibody [6.F1] (AB106095)

ab106095 at 20μg/ml staining STAT5a in Formalin-fixed, Paraffin-embedded Human breast tissue by Immunohistochemistry.
The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Western blot - Anti-STAT5a (phospho Y694) antibody [6.F1] (AB106095)
  • WB

Unknown

Western blot - Anti-STAT5a (phospho Y694) antibody [6.F1] (AB106095)

All lanes:

Western blot - Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095) at 1/1000 dilution

Lane 1:

NK92 cell lysate, untreated

Lane 2:

NK92 cell lysate, treated with 1Ku of IL2 (30min)

Secondary

All lanes:

Gt-a-Mouse IgG DyLight™800 conjugated at 1/20000 dilution

Predicted band size: 105 kDa,11 kDa,112 kDa,115 kDa,122 kDa,140 kDa,15 kDa,17 kDa,18 kDa,201 kDa,24 kDa,26 kDa,29 kDa,32 kDa,351 kDa,36 kDa,38 kDa,39 kDa,43 kDa,47 kDa,48 kDa,52 kDa,54 kDa,57 kDa,58 kDa,67 kDa,69 kDa,71 kDa,73 kDa,85 kDa,88 kDa,90 kDa

Observed band size: 100 kDa,105 kDa,110 kDa,116 kDa,174 kDa,18 kDa,180 kDa,200-220 kDa,24 kDa,26 kDa,30 kDa,35 kDa,36 kDa,370 kDa,39 kDa,40 kDa,42 kDa,47 kDa,48 kDa,50 kDa,52 kDa,58 kDa,67 kDa,73 kDa,75 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-STAT5a (phospho Y694) antibody [6.F1] (AB106095)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-STAT5a (phospho Y694) antibody [6.F1] (AB106095)

ab106095 staining STAT5a in 3T3 cells by Immunofluorescence (red). Green staining represents tubulin.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6.F1

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Mouse Stat5a phospho Y694. The exact immunogen used to generate this antibody is proprietary information.

P42230

Specificity

A BLAST analysis was used to suggest cross-reactivity with Stat5a from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5a from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "20-40 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Phospho STAT5 pY694 was purified from concentrated tissue culture supernate by Protein A chromatography.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STAT5a also known as Signal Transducer and Activator of Transcription 5A acts as a transcription factor in the cell. STAT5a weighs approximately 90 kDa and expresses in various tissues including the liver muscle and hematopoietic cells. It plays a central role in mediating cellular responses to cytokines and growth factors particularly those in the interleukin-2 (IL-2) family and growth hormone (GH).
Biological function summary

Many systems use STAT5a to regulate cell proliferation differentiation and survival. It often forms a complex with other proteins like STAT5b which is a closely related counterpart and both contribute to similar cellular processes. STAT5a can dimerize upon activation allowing it to move to the cell nucleus to influence gene expression directly.

Pathways

Researchers place STAT5a prominently in the JAK-STAT signaling pathway. This pathway is essential for transmitting information received from extracellular chemical signals to the cell nucleus resulting in the activation of genes controlling cellular functions. STAT5a works together with Janus kinases (JAKs) to mediate various biological responses including hormone signaling and immune function. It also interacts with proteins like IL-2 receptor subunits which are involved in many cytokine-driven processes.

Disturbances in STAT5a activity link specifically with conditions like leukemia and breast cancer. Overactivation or mutation can lead to uncontrolled cell growth and survival contributing to these diseases. In leukemia STAT5a frequently partners with other proteins like BCR-ABL which is known for its role in chronic myeloid leukemia influencing oncogenic pathways. In breast cancer alterations in STAT5a signaling may interact with estrogen and progesterone receptors affecting tumor progression and response to therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Carries out a dual function : signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
See full target information STAT5A phospho Y694
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22336 PubMed35522243

2022

Canonical and noncanonical regulatory roles for JAK2 in the pathogenesis of rheumatoid arthritis-associated interstitial lung disease and idiopathic pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shaohua Wang,Mengfei Liu,Xiujuan Li,Jie Zhang,Faping Wang,Chujie Zhang,Anja Roden,Jay H Ryu,Kenneth J Warrington,Jie Sun,Eric L Matteson,Daniel J Tschumperlin,Robert Vassallo

Cytotechnology 67:223-35 PubMed24468831

2014

Modulation of heparan sulfate biosynthesis by sodium butyrate in recombinant CHO cells.

Applications

Unspecified application

Species

Unspecified reactive species

Payel Datta,Bo Yang,Robert J Linhardt,Susan T Sharfstein
View all publications
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