AB235934

Anti-STAT5b antibody

Name: Anti-STAT5b antibody (ab235934) | Abcam
Brand: Abcam Limited
SKU: AB235934
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal STAT5b antibody. Suitable for ChIP, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human STAT5B aa 1-300.

View Alternative Names

Signal transducer and activator of transcription 5B, STAT5B

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody (AB235934)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody (AB235934)

Paraffin-embedded human breast cancer tissue stained for STAT5b using ab235934 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody (AB235934)

Paraffin-embedded human placenta tissue stained for STAT5b using ab235934 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody (AB235934)

Paraffin-embedded human testis tissue stained for STAT5b using ab235934 at 1/100 dilution in immunohistochemical analysis.

ChIP

Unknown

ChIP - Anti-STAT5b antibody (AB235934)

ChIP analysis on chromatin prepared from MCF7 (human breast adenocarcinoma cell line) cells. 1.1 x 10⁶ cells were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4μg ab235934 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the AGT promoter.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ChIP

applications

Immunogen

Recombinant Fragment Protein within Human STAT5B aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P51692

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "Use 4 μg for 1.1 x 106 cells.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STAT5b also known as Signal Transducer and Activator of Transcription 5B is a 92-kDa protein involved in signaling processes within the cell. Mechanically it functions as a transcription factor and shuttles between the cytoplasm and nucleus modulating gene expression according to extracellular signals. Upon cytokine binding STAT5b undergoes phosphorylation allowing it to form dimers and translocate to the nucleus. It is expressed in many tissues including the liver immune cells and mammary glands reflecting its role in diverse physiological processes.

Biological function summary

STAT5b plays an important role in regulating cell growth differentiation and apoptosis. It often acts as part of the STAT protein family binding to specific DNA sequences to activate transcription. STAT5b particularly influences immune function and hematopoiesis driving proliferation and differentiation of lymphoid and myeloid cells. Its activity affects glucose homeostasis growth hormone signaling and lactation highlighting its importance in various physiological states and developmental processes.

Pathways

STAT5b integrates into the JAK-STAT signaling pathway a critical route for conveying extracellular information to the nucleus. This pathway includes JAK kinases which activate STAT5b by phosphorylation upon cytokine-receptor engagement. STAT5b also participates in the growth hormone pathway interacting with proteins such as Growth Hormone Receptor and other STAT family members like STAT3. Through these interactions STAT5b helps regulate gene expression associated with growth and differentiation in response to hormonal signals.

STAT5b links to several conditions such as growth hormone insensitivity and leukemia. In growth disorders defects in STAT5b can impair normal growth hormone signal transduction often involving interplay with proteins like the Growth Hormone Receptor. In leukemias aberrant activation or expression of STAT5b contributes to uncontrolled cellular proliferation often accompanied by deregulation of the JAK-STAT pathway. Research into STAT5b offers insights into potential therapeutic targets for these and other related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Carries out a dual function : signal transduction and activation of transcription (PubMed : 29844444). Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation.

See full target information STAT5B

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 16:79 PubMed37085900

2023

Gab2 plays a carcinogenic role in ovarian cancer by regulating CrkII.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Yin,Li Zhang,Yong Li,Can Zhang,Aiqin He

EMBO molecular medicine 14:e15200 PubMed36341492

2022

Blocking STAT3/5 through direct or upstream kinase targeting in leukemic cutaneous T-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Helena Sorger,Saptaswa Dey,Pablo Augusto Vieyra-Garcia,Daniel Pölöske,Andrea R Teufelberger,Elvin D de Araujo,Abootaleb Sedighi,Ricarda Graf,Benjamin Spiegl,Isaac Lazzeri,Till Braun,Ines Garces de Los Fayos Alonso,Michaela Schlederer,Gerald Timelthaler,Petra Kodajova,Christine Pirker,Marta Surbek,Michael Machtinger,Thomas Graier,Isabella Perchthaler,Yi Pan,Regina Fink-Puches,Lorenzo Cerroni,Jennifer Ober,Moritz Otte,Jana D Albrecht,Gary Tin,Ayah Abdeldayem,Pimyupa Manaswiyoungkul,Olasunkanmi O Olaoye,Martin L Metzelder,Anna Orlova,Walter Berger,Marion Wobser,Jan P Nicolay,Fiona André,Van Anh Nguyen,Heidi A Neubauer,Roman Fleck,Olaf Merkel,Marco Herling,Ellen Heitzer,Patrick T Gunning,Lukas Kenner,Richard Moriggl,Peter Wolf

The Journal of allergy and clinical immunology 144:1025-1035.e7 PubMed31251950

2019

Thymic stromal lymphopoietin epigenetically upregulates Fc receptor γ subunit-related receptors on antigen-presenting cells and induces T2/T17 polarization through dectin-2.

Applications

Unspecified application

Species

Unspecified reactive species

Yunsheng Liang,Bihui Yu,Junchen Chen,Haijin Wu,Yingping Xu,Bin Yang,Qianjin Lu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-STAT5b antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody (AB235934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody (AB235934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody (AB235934)

ChIP - Anti-STAT5b antibody (AB235934)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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