AB52211

Anti-STAT5b (phospho S731) antibody

Name: Anti-STAT5b (phospho S731) antibody (ab52211) | Abcam
Brand: Abcam Limited
SKU: AB52211
Price: 535 USD
Availability: InStock

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(10 Publications)

Rabbit Polyclonal STAT5b phospho S731 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human STAT5B phospho S731 aa 650-750.

View Alternative Names

Signal transducer and activator of transcription 5B, STAT5B

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b (phospho S731) antibody (AB52211)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b (phospho S731) antibody (AB52211)

Paraffin-embedded human breast carcinoma tissue stained for STAT5b (phospho S731), using ab52211 (1/100). The right hand panel represents a negative control where ab52211 was pre-incubated with the immunising (blocking) peptide.

Immunocytochemistry/ Immunofluorescence - Anti-STAT5b (phospho S731) antibody (AB52211)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-STAT5b (phospho S731) antibody (AB52211)

ICC/IF image of ab52211 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52211, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-STAT5b (phospho S731) antibody (AB52211)

All lanes:

Western blot - Anti-STAT5b (phospho S731) antibody (ab52211) at 1/500 dilution

Lane 1:

Extracts from RAW264.7 cells treated with EGF (200ng/ml, 30min)

Lane 2:

Extracts from RAW264.7 cells treated with EGF (200ng/ml, 30min), ab52211 was pre-incubated with immunising (blocking) peptide

Predicted band size: 89 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human STAT5B phospho S731 aa 650-750. The exact immunogen used to generate this antibody is proprietary information.

P51692

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab52211 detects endogenous levels of STAT5B but only when phosphorylated at serine 731 (human) or serine 730 (mouse and rat).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 6 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STAT5b also known as Signal Transducer and Activator of Transcription 5B is a 92-kDa protein involved in signaling processes within the cell. Mechanically it functions as a transcription factor and shuttles between the cytoplasm and nucleus modulating gene expression according to extracellular signals. Upon cytokine binding STAT5b undergoes phosphorylation allowing it to form dimers and translocate to the nucleus. It is expressed in many tissues including the liver immune cells and mammary glands reflecting its role in diverse physiological processes.

Biological function summary

STAT5b plays an important role in regulating cell growth differentiation and apoptosis. It often acts as part of the STAT protein family binding to specific DNA sequences to activate transcription. STAT5b particularly influences immune function and hematopoiesis driving proliferation and differentiation of lymphoid and myeloid cells. Its activity affects glucose homeostasis growth hormone signaling and lactation highlighting its importance in various physiological states and developmental processes.

Pathways

STAT5b integrates into the JAK-STAT signaling pathway a critical route for conveying extracellular information to the nucleus. This pathway includes JAK kinases which activate STAT5b by phosphorylation upon cytokine-receptor engagement. STAT5b also participates in the growth hormone pathway interacting with proteins such as Growth Hormone Receptor and other STAT family members like STAT3. Through these interactions STAT5b helps regulate gene expression associated with growth and differentiation in response to hormonal signals.

STAT5b links to several conditions such as growth hormone insensitivity and leukemia. In growth disorders defects in STAT5b can impair normal growth hormone signal transduction often involving interplay with proteins like the Growth Hormone Receptor. In leukemias aberrant activation or expression of STAT5b contributes to uncontrolled cellular proliferation often accompanied by deregulation of the JAK-STAT pathway. Research into STAT5b offers insights into potential therapeutic targets for these and other related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Carries out a dual function : signal transduction and activation of transcription (PubMed : 29844444). Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation.

See full target information STAT5B phospho S731

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 20:835 PubMed40999516

2025

Neuregulin 4 inhibits synovial macrophage pro-inflammatory polarization via ErbB4/Stat5b/NF-κB signaling to alleviate osteoarthritis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Daoliang Jiang,Xiaoli Xu,Wenjing Yang,Mengjia Tang,Yuping Zhang,Lingfeng Shi,Jiongyu Hu

Iranian journal of basic medical sciences 27:685-694 PubMed38645489

2024

Quercetin of huoxuehuayu tongluo decoction and azithromycin combination therapy effectively improves rat tubal factor infertility by inhibiting inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Shao,Nansu Wang,Yan Yan,Yali Tan,Qin Wu,Lei Lei,Mingming Wang,Ling Liu

Proceedings of the National Academy of Sciences of the United States of America 119:e2213628119 PubMed36442127

2022

Deletion of gene causes early growth retardation and insulin resistance in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Young Lee,Hye Rim Jang,Hui Li,Varman T Samuel,Karrie D Dudek,Anna B Osipovich,Mark A Magnuson,Jeffrey Sklar,Gerald I Shulman

EBioMedicine 86:104312 PubMed36335669

2022

Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shihui Xu,Xiaobing Yang,Qingzhou Chen,Zhuoliang Liu,Ying Chen,Xiaotian Yao,An Xiao,Jianwei Tian,Liling Xie,Miaomiao Zhou,Zheng Hu,Fengxin Zhu,Xin Xu,Fanfan Hou,Jing Nie

International journal of oncology 61: PubMed36069226

2022

STAT5b is a marker of poor prognosis, rather than a therapeutic target in glioblastomas.

Applications

Unspecified application

Species

Unspecified reactive species

Nadège Dubois,Sharon Berendsen,Katherine Tan,Laurent Schoysmans,Wim Spliet,Tatjana Seute,Vincent Bours,Pierre A Robe

Cell communication and signaling : CCS 18:107 PubMed32646440

2020

MKL-1 is a coactivator for STAT5b, the regulator of Treg cell development and function.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Xiang,Jun Wang,Jia Peng Li,Wei Guo,Feng Huang,Hui Min Zhang,Han Han Li,Zhou Tong Dai,Zi Jian Zhang,Hui Li,Le Yuan Bao,Chao Jiang Gu,Kun Chen,Tong Cun Zhang,Xing Hua Liao

Journal of Cancer 11:2222-2233 PubMed32127949

2020

The Role of MiR-5094 as a Proliferation Suppressor during Cellular Radiation Response via Downregulating STAT5b.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Ding,Junrui Hua,Jinpeng He,Dong Lu,Wenjun Wei,Yanan Zhang,Heng Zhou,Liying Zhang,Yongqi Liu,Guangming Zhou,Jufang Wang

Cancer management and research 11:6625-6635 PubMed31406476

2019

HOXA10 deteriorates gastric cancer through activating JAK1/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenchao Chen,Gang Wu,Yuanzeng Zhu,Wei Zhang,Han Zhang,Yang Zhou,Peichun Sun

Endocrinology 158:3954-3973 PubMed28973305

2017

Metabolic, Reproductive, and Neurologic Abnormalities in Agpat1-Null Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Anil K Agarwal,Katie Tunison,Jasbir S Dalal,Sneha S Nagamma,F Kent Hamra,Shireesha Sankella,Xinli Shao,Richard J Auchus,Abhimanyu Garg

PLoS pathogens 7:e1002088 PubMed21698228

2011

Productive parvovirus B19 infection of primary human erythroid progenitor cells at hypoxia is regulated by STAT5A and MEK signaling but not HIFα.

Applications

Species

Human

Aaron Yun Chen,Steve Kleiboeker,Jianming Qiu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-STAT5b (phospho S731) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b (phospho S731) antibody (AB52211)

Immunocytochemistry/ Immunofluorescence - Anti-STAT5b (phospho S731) antibody (AB52211)

Western blot - Anti-STAT5b (phospho S731) antibody (AB52211)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise