Rabbit Polyclonal STAT5b phospho S731 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human STAT5B phospho S731 aa 650-750.
Images
Publications
Filter by
- Proceedings of the National Academy of Sciences of the United States of America 119:e22136281192022Deletion of gene causes early growth retardation and insulin resistance in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui-Young Lee,Hye Rim Jang,Hui Li,Varman T Samuel,Karrie D Dudek,Anna B Osipovich,Mark A Magnuson,Jeffrey Sklar,Gerald I ShulmanPubMed 36442127
- EBioMedicine 86:1043122022Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShihui Xu,Xiaobing Yang,Qingzhou Chen,Zhuoliang Liu,Ying Chen,Xiaotian Yao,An Xiao,Jianwei Tian,Liling Xie,Miaomiao Zhou,Zheng Hu,Fengxin Zhu,Xin Xu,Fanfan Hou,Jing NiePubMed 36335669
- International journal of oncology 61:2022STAT5b is a marker of poor prognosis, rather than a therapeutic target in glioblastomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNadège Dubois,Sharon Berendsen,Katherine Tan,Laurent Schoysmans,Wim Spliet,Tatjana Seute,Vincent Bours,Pierre A RobePubMed 36069226
- Cell communication and signaling : CCS 18:1072020MKL-1 is a coactivator for STAT5b, the regulator of Treg cell development and function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Xiang et. al.PubMed 32646440
- Journal of Cancer 11:2222-22332020The Role of MiR-5094 as a Proliferation Suppressor during Cellular Radiation Response via Downregulating STAT5b.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNan Ding et. al.PubMed 32127949
- Cancer management and research 11:6625-66352019HOXA10 deteriorates gastric cancer through activating JAK1/STAT3 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenchao Chen et. al.PubMed 31406476
- Endocrinology 158:3954-39732017Metabolic, Reproductive, and Neurologic Abnormalities in Agpat1-Null Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnil K Agarwal,Katie Tunison,Jasbir S Dalal,Sneha S Nagamma,F Kent Hamra,Shireesha Sankella,Xinli Shao,Richard J Auchus,Abhimanyu GargPubMed 28973305
- PLoS pathogens 7:e10020882011Productive parvovirus B19 infection of primary human erythroid progenitor cells at hypoxia is regulated by STAT5A and MEK signaling but not HIFα.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human STAT5B phospho S731 aa 650-750. The exact immunogen used to generate this antibody is proprietary information. Database link P51692
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Carries out a dual function: signal transduction and activation of transcription (PubMed:29844444). Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation.
Alternative names
Signal transducer and activator of transcription 5B, STAT5B
Recommended products
Rabbit Polyclonal STAT5b phospho S731 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human STAT5B phospho S731 aa 650-750.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human STAT5B phospho S731 aa 650-750. The exact immunogen used to generate this antibody is proprietary information. Database link P51692
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 6 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
ab52211 detects endogenous levels of STAT5B but only when phosphorylated at serine 731 (human) or serine 730 (mouse and rat).
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
STAT5b also known as Signal Transducer and Activator of Transcription 5B is a 92-kDa protein involved in signaling processes within the cell. Mechanically it functions as a transcription factor and shuttles between the cytoplasm and nucleus modulating gene expression according to extracellular signals. Upon cytokine binding STAT5b undergoes phosphorylation allowing it to form dimers and translocate to the nucleus. It is expressed in many tissues including the liver immune cells and mammary glands reflecting its role in diverse physiological processes.
- Biological function summary
STAT5b plays an important role in regulating cell growth differentiation and apoptosis. It often acts as part of the STAT protein family binding to specific DNA sequences to activate transcription. STAT5b particularly influences immune function and hematopoiesis driving proliferation and differentiation of lymphoid and myeloid cells. Its activity affects glucose homeostasis growth hormone signaling and lactation highlighting its importance in various physiological states and developmental processes.
- Pathways
STAT5b integrates into the JAK-STAT signaling pathway a critical route for conveying extracellular information to the nucleus. This pathway includes JAK kinases which activate STAT5b by phosphorylation upon cytokine-receptor engagement. STAT5b also participates in the growth hormone pathway interacting with proteins such as Growth Hormone Receptor and other STAT family members like STAT3. Through these interactions STAT5b helps regulate gene expression associated with growth and differentiation in response to hormonal signals.
- Associated diseases and disorders
STAT5b links to several conditions such as growth hormone insensitivity and leukemia. In growth disorders defects in STAT5b can impair normal growth hormone signal transduction often involving interplay with proteins like the Growth Hormone Receptor. In leukemias aberrant activation or expression of STAT5b contributes to uncontrolled cellular proliferation often accompanied by deregulation of the JAK-STAT pathway. Research into STAT5b offers insights into potential therapeutic targets for these and other related conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-STAT5b (phospho S731) antibody (ab52211)
All lanes: Western blot - Anti-STAT5b (phospho S731) antibody (ab52211) at 1/500 dilution
Lane 1: Extracts from RAW264.7 cells treated with EGF (200ng/ml, 30min)
Lane 2: Extracts from RAW264.7 cells treated with EGF (200ng/ml, 30min), ab52211 was pre-incubated with immunising (blocking) peptide
Predicted band size: 89 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b (phospho S731) antibody (ab52211)
Paraffin-embedded human breast carcinoma tissue stained for STAT5b (phospho S731), using ab52211 (1/100). The right hand panel represents a negative control where ab52211 was pre-incubated with the immunising (blocking) peptide.
Immunocytochemistry/ Immunofluorescence - Anti-STAT5b (phospho S731) antibody (ab52211)
ICC/IF image of ab52211 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52211, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com