AB277765

Anti-STAT6 antibody [21HCLC]

Recombinant
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(1 Publication)

Rabbit Recombinant Multiclonal STAT6 antibody. Suitable for ChIP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human STAT6 aa 600-650.

View Alternative Names

Signal transducer and activator of transcription 6, IL-4 Stat, STAT6

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ChIP - Anti-STAT6 antibody [21HCLC] (AB277765)

ChIP

Supplier Data

ChIP - Anti-STAT6 antibody [21HCLC] (AB277765)

ChIP- qPCR analysis of STAT6 was performed with 3 μg/mL of the STAT6 Recombinant Rabbit Polyclonal Antibody (ab277765) on sheared chromatin from 2 million HeLa cells treated with IFN-gamma (50 ng/mL) for 1h using the MAGnify Chromatin Immunoprecipitation System. Normal rabbit IgG (3 μg/mL) was used as a negative IP control. The purified DNA from each ChIP sample was analyzed by StepOnePlus Real-Time PCR System with primers for the promoter of active AP-1 and COX2 gene, used as positive control targets, and the inactive SAT2 satellite repeat, used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

Supplier Data

Western blot - Anti-STAT6 antibody [21HCLC] (AB277765)

Western blot analysis of STAT6 in whole cell extracts of serum-starved HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with IFN1a (150 ng/mL, 15 min) using a STAT6 Recombinant Rabbit Polyclonal Antibody (ab277765) at a dilution of 1 μg/mL.

All lanes:

Western blot - Anti-STAT6 antibody [21HCLC] (ab277765) at 1 µg/mL

All lanes:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with IFN1a (150 ng/mL, 15 min)

Predicted band size: 94 kDa

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Supplier Data

Western blot - Anti-STAT6 antibody [21HCLC] (AB277765)

All lanes:

Western blot - Anti-STAT6 antibody [21HCLC] (ab277765) at 1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Lane 3:

A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG-HRP at 1/5000 dilution

Predicted band size: 94 kDa

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Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

21HCLC

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ChIP, WB

applications

Immunogen

Synthetic Peptide within Human STAT6 aa 600-650. The exact immunogen used to generate this antibody is proprietary information.

P42226

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p>3 μg</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STAT6 also known as Signal Transducer and Activator of Transcription 6 is a transcription factor with a mass of around 94 kDa. This protein is an important component in the signaling pathways that respond to cytokines especially those of the IL-4 and IL-13 receptors. STAT6 mainly expresses in immune cells including T cells B cells and macrophages. It exists in the cytoplasm until the cytokine stimulation prompts its phosphorylation and translocation to the nucleus where it influences gene expression.

Biological function summary

STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.

Pathways

STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.

STAT6 has associations with asthma and certain types of cancer such as colorectal cancer. In asthma STAT6 influences the production of IgE and eosinophilic inflammation often connected to allergic responses. Meanwhile STAT6 promotes tumorigenesis in some cancers by regulating genes responsible for cell survival and proliferation. It connects with proteins like IL-4 and IL-13 in these disorders which suggests potential therapeutic targets for treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Carries out a dual function : signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

See full target information STAT6

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Endokrynologia Polska 73:121-130 PubMed34855195

2021

Biological functions of nuclear-localized insulin receptor (IR) on A549 lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ren Qiu,Tian Miao,Lin Lin,Zhang Wei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-STAT6 antibody [21HCLC]

ChIP - Anti-STAT6 antibody [21HCLC] (AB277765)

Western blot - Anti-STAT6 antibody [21HCLC] (AB277765)

Western blot - Anti-STAT6 antibody [21HCLC] (AB277765)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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