Anti-STAT6 antibody [23/Stat6] - BSA and Azide free

• WB

Lab

Western blot - Anti-STAT6 antibody [23/Stat6] - BSA and Azide free (AB282127)

This data was developed using 282107, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 92 seconds

All lanes:

Western blot - Anti-STAT6 antibody [23/Stat6] (<a href='/en-us/products/primary-antibodies/stat6-antibody-23-stat6-ab282107'>ab282107</a>) at 1/1000 dilution

Lane 1:

Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 3:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysat at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 94 kDa

false

• WB

Lab

Western blot - Anti-STAT6 antibody [23/Stat6] - BSA and Azide free (AB282127)

This data was developed using 282107, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS

Lanes 1-2 : Merged signal (red and green). Green - ab282107 observed at 94kDa. Red - loading control ab181602 (Rabbit monoclonal [EPR16891] to GAPDH) observed at 36 kDa.

Lanes 1-2 : ab282107 Anti-Stat6 antibody was shown to react with STAT6 in HAP1 cells in Western blot. Loss of signal was observed when STAT6 knockout sample was used. Wild-type and STAT6 knockout samples were subjected to SDS-PAGE.

ab282107 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated at 4℃ overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800RD) preadsorbed (ab216772) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-STAT6 antibody [23/Stat6] (<a href='/en-us/products/primary-antibodies/stat6-antibody-23-stat6-ab282107'>ab282107</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

Lane 2:

STAT6 knockout HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Mouse IgG H&L (IRDye® 800CW) (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-800cw-preadsorbed-ab216772'>ab216772</a>) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/10000 dilution

Predicted band size: 94 kDa

false