AB88540

Anti-STAT6 antibody

Name: Anti-STAT6 antibody (ab88540) | Abcam
Brand: Abcam Limited
SKU: AB88540
Price: 545 USD
Availability: InStock

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(3 Publications)

Mouse Polyclonal STAT6 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human STAT6.

View Alternative Names

Signal transducer and activator of transcription 6, IL-4 Stat, STAT6

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody (AB88540)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody (AB88540)

Immunofluorescence of ab88540 on HeLa cell with antibody concentration at 10 ug/ml

Unknown

Western blot - Anti-STAT6 antibody (AB88540)

All lanes:

Western blot - Anti-STAT6 antibody (ab88540) at 1 µg/mL

Lane 1:

STAT6 transfected 293T cell lysate at 50 µg

Lane 2:

Non-transfected 293T cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 94 kDa

Observed band size: 100 kDa

false

Unknown

Western blot - Anti-STAT6 antibody (AB88540)

All lanes:

Western blot - Anti-STAT6 antibody (ab88540) at 1 µg/mL

All lanes:

Human pancreas tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

Predicted band size: 94 kDa

Observed band size: 100 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human STAT6.

P42226

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STAT6 also known as Signal Transducer and Activator of Transcription 6 is a transcription factor with a mass of around 94 kDa. This protein is an important component in the signaling pathways that respond to cytokines especially those of the IL-4 and IL-13 receptors. STAT6 mainly expresses in immune cells including T cells B cells and macrophages. It exists in the cytoplasm until the cytokine stimulation prompts its phosphorylation and translocation to the nucleus where it influences gene expression.

Biological function summary

STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.

Pathways

STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.

STAT6 has associations with asthma and certain types of cancer such as colorectal cancer. In asthma STAT6 influences the production of IgE and eosinophilic inflammation often connected to allergic responses. Meanwhile STAT6 promotes tumorigenesis in some cancers by regulating genes responsible for cell survival and proliferation. It connects with proteins like IL-4 and IL-13 in these disorders which suggests potential therapeutic targets for treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Carries out a dual function : signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

See full target information STAT6

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Immunology 166:47-67 PubMed35143048

2022

Staphylococcus aureus controls eicosanoid and specialized pro-resolving mediator production via lipoteichoic acid.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Miek,Paul M Jordan,Kerstin Günther,Simona Pace,Timo Beyer,David Kowalak,Verena Hoerr,Bettina Löffler,Lorena Tuchscherr,Charles N Serhan,Jana Gerstmeier,Oliver Werz

Journal of immunology (Baltimore, Md. : 1950) 203:1031-1043 PubMed31300512

2019

Vacuolar (H)-ATPase Critically Regulates Specialized Proresolving Mediator Pathways in Human M2-like Monocyte-Derived Macrophages and Has a Crucial Role in Resolution of Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhigang Rao,Simona Pace,Paul M Jordan,Rossella Bilancia,Fabiana Troisi,Friedemann Börner,Nico Andreas,Thomas Kamradt,Dirk Menche,Antonietta Rossi,Charles N Serhan,Jana Gerstmeier,Oliver Werz

Frontiers in physiology 10:143 PubMed30863317

2019

Dynamin Binding Protein Is Required for Kidney Development.

Applications

Unspecified application

Species

Unspecified reactive species

Bridget D DeLay,Tanya A Baldwin,Rachel K Miller

View all publications

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Product promise

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