AB217998

Anti-STAT6 antibody [EPR18278-222]

20ul selling size
RabMAb
Recombinant
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(3 Publications )

Rabbit Recombinant Monoclonal STAT6 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

Signal transducer and activator of transcription 6, IL-4 Stat, STAT6

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR18278-222] (AB217998)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR18278-222] (AB217998)

Immunofluorescent analysis of 100% methanol-fixed Raji (Human Burkitt's lymphoma cell line) cells labeling STAT6 with ab217998 at 1/500 dilution followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining on Raji cell line [PMID : 11781246] [PMID : 23575898].

The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR18278-222] (AB217998)

Immunofluorescent analysis of 100% methanol-fixed Daudi (Human Burkitt's lymphoma cell line) cells labeling STAT6 with ab217998 at 1/500 dilution followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining on Daudi cell line.

The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-STAT6 antibody [EPR18278-222] (AB217998)

STAT6 was immunoprecipitated from 0.35 mg of Raji (Human Burkitt's lymphoma cell line) whole cell lysate with ab217998 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab217998 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Raji whole cell lysate, 10 μg (Input).

Lane 2 : ab217998 IP in Raji whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab217998 in Raji whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

This antibody recognizes three isoforms of STAT6, isoform 1 (110kDa), isoform 2 (75kDa), isoform 3 (81kDa), respectively which is consistent with the Uniprot annotation.

All lanes:

Immunoprecipitation - Anti-STAT6 antibody [EPR18278-222] (ab217998)

Predicted band size: 94 kDa

Observed band size: 110 kDa,75 kDa,81 kDa

false

Supplier Data

Western blot - Anti-STAT6 antibody [EPR18278-222] (AB217998)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1,2 and 3 : 8 seconds; Lane 4 and 5 : 2 seconds; Lane 6 and 7 : 1 second.

The molecular weight observed is consistent with what has been described in the literature (PMID 21411736). This antibody recognizes three isoforms of STAT6, isoform 1 (110kDa), isoform 2 (75kDa), isoform 3 (81kDa), which is consistent with the Uniprot annotation.

All lanes:

Western blot - Anti-STAT6 antibody [EPR18278-222] (ab217998) at 1/1000 dilution

Lane 1:

Human thymus lysate at 20 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

P388D1 (Mouse lymphoma N106 O91monocyte; macrophage cell line) whole cell lysate at 20 µg

Lane 4:

Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 5:

THP-1 (Human monocytic leukemia cell line) whole cell lysate at 20 µg

Lane 6:

Daudi (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 7:

Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 94 kDa

Observed band size: 110 kDa,75 kDa,81 kDa

false

Supplier Data

Western blot - Anti-STAT6 antibody [EPR18278-222] (AB217998)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 10 seconds; Lane 2 : 3 seconds; Lane 3 : 5 seconds.

All lanes:

Western blot - Anti-STAT6 antibody [EPR18278-222] (ab217998) at 1/2000 dilution

Lane 1:

Mouse spleen tissue lysate at 10 µg

Lane 2:

Rat spleen tissue lysate at 10 µg

Lane 3:

A20 (Mouse reticulum sarcoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 94 kDa

Observed band size: 110 kDa,75 kDa,81 kDa

false

Carrier free

Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18278-222

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STAT6 also known as Signal Transducer and Activator of Transcription 6 is a transcription factor with a mass of around 94 kDa. This protein is an important component in the signaling pathways that respond to cytokines especially those of the IL-4 and IL-13 receptors. STAT6 mainly expresses in immune cells including T cells B cells and macrophages. It exists in the cytoplasm until the cytokine stimulation prompts its phosphorylation and translocation to the nucleus where it influences gene expression.

Biological function summary

STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.

Pathways

STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.

STAT6 has associations with asthma and certain types of cancer such as colorectal cancer. In asthma STAT6 influences the production of IgE and eosinophilic inflammation often connected to allergic responses. Meanwhile STAT6 promotes tumorigenesis in some cancers by regulating genes responsible for cell survival and proliferation. It connects with proteins like IL-4 and IL-13 in these disorders which suggests potential therapeutic targets for treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Carries out a dual function : signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

See full target information STAT6

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 12: PubMed36670936

2022

Specificity Protein 1-Mediated Promotion of CXCL12 Advances Endothelial Cell Metabolism and Proliferation in Pulmonary Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Evan R DeVallance,Christopher M Dustin,Daniel Simoes de Jesus,Imad Al Ghouleh,John C Sembrat,Eugenia Cifuentes-Pagano,Patrick J Pagano

Frontiers in aging neuroscience 14:883503 PubMed35663575

2022

Mesenchymal Stem Cells Improve Cognitive Impairment and Reduce Aβ Deposition Promoting AQP4 Polarity and Relieving Neuroinflammation in Rats With Chronic Hypertension-Induced Cerebral Small-Vessel Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Lu Liu,Fu Bing Ouyang,Liu Ting Hu,Pei Sun,Jing Yang,Yuan Jing Sun,Meng Shi Liao,Lin Fang Lan,Zhong Pei,Yu Hua Fan

BMC medicine 20:148 PubMed35422042

2022

Ketone body β-hydroxybutyrate ameliorates colitis by promoting M2 macrophage polarization through the STAT6-dependent signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chongyang Huang,Jun Wang,Hongbin Liu,Ruo Huang,Xinwen Yan,Mengyao Song,Gao Tan,Fachao Zhi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-STAT6 antibody [EPR18278-222]

Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR18278-222] (AB217998)

Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR18278-222] (AB217998)

Immunoprecipitation - Anti-STAT6 antibody [EPR18278-222] (AB217998)

Western blot - Anti-STAT6 antibody [EPR18278-222] (AB217998)

Western blot - Anti-STAT6 antibody [EPR18278-222] (AB217998)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise