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Rabbit Recombinant Monoclonal STAT6 antibody. Suitable for WB, ICC/IF and reacts with Human samples.

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Images

Western blot - Anti-STAT6 antibody [EPR1912] (AB134940), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR1912] (AB134940), expandable thumbnail
  • Western blot - Anti-STAT6 antibody [EPR1912] (AB134940), expandable thumbnail
  • Western blot - Anti-STAT6 antibody [EPR1912] (AB134940), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IF
Human
Not recommended
Tested
Not recommended
Tested

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

Alternative names

Signal transducer and activator of transcription 6, IL-4 Stat, STAT6

Recommended products

Rabbit Recombinant Monoclonal STAT6 antibody. Suitable for WB, ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR1912
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

STAT6 also known as Signal Transducer and Activator of Transcription 6 is a transcription factor with a mass of around 94 kDa. This protein is an important component in the signaling pathways that respond to cytokines especially those of the IL-4 and IL-13 receptors. STAT6 mainly expresses in immune cells including T cells B cells and macrophages. It exists in the cytoplasm until the cytokine stimulation prompts its phosphorylation and translocation to the nucleus where it influences gene expression.

Biological function summary

STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.

Pathways

STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.

Associated diseases and disorders

STAT6 has associations with asthma and certain types of cancer such as colorectal cancer. In asthma STAT6 influences the production of IgE and eosinophilic inflammation often connected to allergic responses. Meanwhile STAT6 promotes tumorigenesis in some cancers by regulating genes responsible for cell survival and proliferation. It connects with proteins like IL-4 and IL-13 in these disorders which suggests potential therapeutic targets for treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-STAT6 antibody [EPR1912] (ab134940), expandable thumbnail

    Western blot - Anti-STAT6 antibody [EPR1912] (ab134940)

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
    Lane 2: STAT6 knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)
    Lane 4: Raji whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab134940 observed at 94 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab134940 was shown to recognize STAT6 in wild-type HAP1 cells as signal was lost at the expected MW in STAT6 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and STAT6 knockout samples were subjected to SDS-PAGE. ab134940 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-STAT6 antibody [EPR1912] (ab134940)

    Predicted band size: 94 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR1912] (ab134940), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR1912] (ab134940)

    Immunofluorescence analysis of Daudi cells, labelling STAT6 using ab134940 at a 1/100 dilution (green). Cell nuclei are shown in blue.

  • Western blot - Anti-STAT6 antibody [EPR1912] (ab134940), expandable thumbnail

    Western blot - Anti-STAT6 antibody [EPR1912] (ab134940)

    All lanes: Western blot - Anti-STAT6 antibody [EPR1912] (ab134940) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: Human lymph node lysate at 10 µg

    Lane 3: Daudi cell lysate at 10 µg

    Lane 4: Raji cell lysate at 10 µg

    Secondary

    All lanes: Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 94 kDa

  • Western blot - Anti-STAT6 antibody [EPR1912] (ab134940), expandable thumbnail

    Western blot - Anti-STAT6 antibody [EPR1912] (ab134940)

    STAT6 Western blot staining of Wild-type A549 using rabbit Anti-STAT6 antibody

    Western blot: Recombinant Anti-STAT6 antibody [EPR1912] ab134940 staining at 1/5000 dilution, shown in green; Mouse anti-CANX (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 100 kDa in Wild-type A549 cell lysates with no signal observed at this size in STAT6 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

    All lanes: Western blot - Anti-STAT6 antibody [EPR1912] (ab134940) at 1/5000 dilution

    Lane 1: Wild-type A549 at 20 µg

    Lane 2: Western blot - Human STAT6 knockout A549 cell line (Human STAT6 knockout A549 cell line ab286716) at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 94 kDa

    Observed band size: 100 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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