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Rabbit Recombinant Monoclonal STAT6 antibody. Suitable for ChIC/CUT&RUN-seq, IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 68 publications.


Images

Immunoprecipitation - Anti-STAT6 antibody [YE361] (AB32520), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (AB32520), expandable thumbnail
  • Western blot - Anti-STAT6 antibody [YE361] (AB32520), expandable thumbnail
  • Western blot - Anti-STAT6 antibody [YE361] (AB32520), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (AB32520), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIC/CUT&RUN-seqIHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Expected
Tested
Tested
Tested
Mouse
Expected
Tested
Tested
Tested
Expected
Expected

Tested
Tested

Species

Human

Dilution info

5 µg

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Human

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/20 - 1/80

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/2000

Notes

-

Species

Human

Dilution info

1/1000 - 1/2000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

14 products for Alternative Product

Target data

Function

Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

Alternative names

Signal transducer and activator of transcription 6, IL-4 Stat, STAT6

Recommended products

Rabbit Recombinant Monoclonal STAT6 antibody. Suitable for ChIC/CUT&RUN-seq, IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 68 publications.

Alternative names

Signal transducer and activator of transcription 6, IL-4 Stat, STAT6

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

YE361

Purification technique

Affinity purification Protein A

Specificity

This antibody does not cross-react with other Stat family members.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

STAT6 also known as Signal Transducer and Activator of Transcription 6 is a transcription factor with a mass of around 94 kDa. This protein is an important component in the signaling pathways that respond to cytokines especially those of the IL-4 and IL-13 receptors. STAT6 mainly expresses in immune cells including T cells B cells and macrophages. It exists in the cytoplasm until the cytokine stimulation prompts its phosphorylation and translocation to the nucleus where it influences gene expression.

Biological function summary

STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.

Pathways

STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.

Associated diseases and disorders

STAT6 has associations with asthma and certain types of cancer such as colorectal cancer. In asthma STAT6 influences the production of IgE and eosinophilic inflammation often connected to allergic responses. Meanwhile STAT6 promotes tumorigenesis in some cancers by regulating genes responsible for cell survival and proliferation. It connects with proteins like IL-4 and IL-13 in these disorders which suggests potential therapeutic targets for treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

  • Immunoprecipitation - Anti-STAT6 antibody [YE361] (ab32520), expandable thumbnail

    Immunoprecipitation - Anti-STAT6 antibody [YE361] (ab32520)

    ab32520 (purified) at 1/20 immunoprecipitating STAT6 in 10 μg NIH/3T3 (mouse embyro fibroblast cell line; Lanes 1 and 2, observed at 100 kDa). Lane 3 - PBS. For western blotting, HRP Veriblot for IP (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-STAT6 antibody [YE361] (ab32520)

    Predicted band size: 94 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520)

    Immunohistochemical staining of paraffin embedded human kidney with purified ab32520 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-STAT6 antibody [YE361] (ab32520), expandable thumbnail

    Western blot - Anti-STAT6 antibody [YE361] (ab32520)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-STAT6 antibody [YE361] (ab32520) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embyro fibroblast cell line) cell lysate at 10 µg

    Lane 2: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size: 94 kDa

    Observed band size: 100 kDa

  • Western blot - Anti-STAT6 antibody [YE361] (ab32520), expandable thumbnail

    Western blot - Anti-STAT6 antibody [YE361] (ab32520)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-STAT6 antibody [YE361] (ab32520) at 1/20000 dilution

    All lanes: Raji (human Burkitt's lymphoma cell line) cell lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size: 94 kDa

    Observed band size: 100 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labelling STAT6 with unpurified ab32520 at a dilution of 1/1000. HRP goat anti-rabbit (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used at a dilution of 1/500. The antigen retrieval solution was Tris-EDTA buffer, pH 9.0.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma tissue labelling STAT6 with unpurified ab32520 at 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [YE361] (ab32520), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [YE361] (ab32520)

    Immunocytochemistry/Immunofluorescence analyis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling STAT6 with unpurified ab32520 at 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human transitional cell carcinoma of bladder tissue sections labelling STAT6 with unpurified ab32520 at a dilution of 1/1000. HRP goat anti-rabbit (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used at a dilution of 1/500. The antigen retrieval solution was Tris-EDTA buffer, pH 9.0.

  • Western blot - Anti-STAT6 antibody [YE361] (ab32520), expandable thumbnail

    Western blot - Anti-STAT6 antibody [YE361] (ab32520)

    All lanes: Western blot - Anti-STAT6 antibody [YE361] (ab32520) at 1/1000 dilution

    All lanes: NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

    Predicted band size: 94 kDa

    Observed band size: 100 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labelling STAT6 with unpurified ab32520 at a dilution of 1/1000. HRP goat anti-rabbit (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used at a dilution of 1/500. The antigen retrieval solution was Tris-EDTA buffer, pH 9.0.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labelling STAT6 with unpurified ab32520 at a dilution of 1/1000. HRP goat anti-rabbit (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used at a dilution of 1/500. The antigen retrieval solution was Tris-EDTA buffer, pH 9.0.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human solitary fibrous tumor tissue sections labelling STAT6 with unpurified ab32520 at a dilution of 1/1000. HRP goat anti-rabbit (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used at a dilution of 1/500. The antigen retrieval solution was Tris-EDTA buffer, pH 9.0.

  • ChIC/CUT&RUN sequencing - Anti-STAT6 antibody [YE361] (ab32520), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-STAT6 antibody [YE361] (ab32520)

    ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) cells and 5µg of ab32520 [YE361]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.

    Additional screenshots of mapped reads can be found in the Protocol booklet in the Support and downloads section.

    The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (ab32520)

    Immunohistochemical analysis of formalin fixed paraffin embedded human kidney labelling STAT6 with ab32520 at a concentration of 0.79µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 min with DISCOVERY cell conditioning solution (CC1)  100°C, pH 8.5. ab32520 was incubated at 37°C for 16 min. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-STAT6 antibody [YE361] (ab32520), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-STAT6 antibody [YE361] (ab32520)

    Intracellular Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling STAT6 with purified ab32520 at 1/30 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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