Rabbit Polyclonal STAT6 phospho Y641 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 37 publications. Immunogen corresponding to Synthetic Peptide within Human STAT6 phospho Y641.
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- Science advances 8:eabo43632022A highly selective JAK3 inhibitor is developed for treating rheumatoid arthritis by suppressing γc cytokine-related JAK-STAT signal.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChengjuan Chen,Yuan Yin,Gaona Shi,Yu Zhou,Shuai Shao,Yazi Wei,Lei Wu,Dayong Zhang,Lan Sun,Tiantai ZhangPubMed 35984890
- Molecular systems biology 18:e108552022Drug-microenvironment perturbations reveal resistance mechanisms and prognostic subgroups in CLL.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeter-Martin Bruch,Holly Ar Giles,Carolin Kolb,Sophie A Herbst,Tina Becirovic,Tobias Roider,Junyan Lu,Sebastian Scheinost,Lena Wagner,Jennifer Huellein,Ivan Berest,Mark Kriegsmann,Katharina Kriegsmann,Christiane Zgorzelski,Peter Dreger,Judith B Zaugg,Carsten Müller-Tidow,Thorsten Zenz,Wolfgang Huber,Sascha DietrichPubMed 35959629
- Cellular and molecular gastroenterology and hepatology 13:1449-14672022CD73 Epithelial Progenitor Cells That Contribute to Homeostasis and Renewal Are Depleted in Eosinophilic Esophagitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakeo Hara,Yuta Kasagi,Joshua Wang,Masaru Sasaki,Bailey Aaron,Adam Karami,Masataka Shimonosono,Rieko Shimonosono,Hisatsugu Maekawa,Lauren Dolinsky,Benjamin Wilkins,Jeremy Klein,Jane Wei,Kathryn Nunes,Kristle Lynch,Jonathan M Spergel,Kathryn E Hamilton,Melanie A Ruffner,Tatiana A Karakasheva,Kelly A Whelan,Hiroshi Nakagawa,Amanda B MuirPubMed 35108658
- Molecular oncology 16:2214-22342021Anti-Chi3L1 antibody suppresses lung tumor growth and metastasis through inhibition of M2 polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi Eun Yu,In Jun Yeo,Dong Ju Son,Jaesuk Yun,Sang-Bae Han,Jin Tae HongPubMed 34861103
- Proceedings of the National Academy of Sciences of the United States of America 118:2021Interleukins 4 and 13 drive lipid abnormalities in skin cells through regulation of sex steroid hormone synthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenlu Zhang,Mahendran Chinnappan,Courtney A Prestwood,Marshall Edwards,Methinee Artami,Bonne M Thompson,Kaitlyn M Eckert,Goncalo Vale,Christos C Zouboulis,Jeffrey G McDonald,Tamia A Harris-TryonPubMed 34521750
- Biomolecules 11:2021JAK2 Phosphorylation Signals and Their Associated Cytokines Involved in Chronic Rhinosinusitis with Nasal Polyps and Correlated with Disease Severity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Tsai Lin,Wei-Chih Chen,Ming-Hsien Tsai,Jing-Ying Chen,Chih-Yen Chien,Shun-Chen HuangPubMed 34356683
- Cancer immunology research 8:1426-14392020Blocking P2X7-Mediated Macrophage Polarization Overcomes Treatment Resistance in Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuliang Qin,Xiaoyu Zhang,Binghe Tan,Su Zhang,Chengcong Yin,Qi Xue,Zhen Zhang,Hua Ren,Jinlian Chen,Mingyao Liu,Min Qian,Bing DuPubMed 32933967
- Drug design, development and therapy 14:3683-36952020LncRNA SNHG4 Attenuates Inflammatory Responses by Sponging miR-449c-5p and Up-Regulating STAT6 in Microglial During Cerebral Ischemia-Reperfusion Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuo Zhang et. al.PubMed 32982175
- Scientific reports 10:139152020Adoptive transfer of bone marrow-derived dendritic cells (BMDCs) alleviates OVA-induced allergic airway inflammation in asthmatic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKan Xu et. al.PubMed 32807859
- Journal of neuroinflammation 17:2402020Thrombomodulin facilitates peripheral nerve regeneration through regulating M1/M2 switching.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTzu-Chieh Huang et. al.PubMed 32799887
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human STAT6 phospho Y641. The exact immunogen used to generate this antibody is proprietary information. Database link P42226
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
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6 products for Alternative Product
Target data
Function
Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.
Alternative names
Signal transducer and activator of transcription 6, IL-4 Stat, STAT6
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Rabbit Polyclonal STAT6 phospho Y641 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 37 publications. Immunogen corresponding to Synthetic Peptide within Human STAT6 phospho Y641.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human STAT6 phospho Y641. The exact immunogen used to generate this antibody is proprietary information. Database link P42226
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
STAT6 also known as Signal Transducer and Activator of Transcription 6 is a transcription factor with a mass of around 94 kDa. This protein is an important component in the signaling pathways that respond to cytokines especially those of the IL-4 and IL-13 receptors. STAT6 mainly expresses in immune cells including T cells B cells and macrophages. It exists in the cytoplasm until the cytokine stimulation prompts its phosphorylation and translocation to the nucleus where it influences gene expression.
- Biological function summary
STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.
- Pathways
STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.
- Associated diseases and disorders
STAT6 has associations with asthma and certain types of cancer such as colorectal cancer. In asthma STAT6 influences the production of IgE and eosinophilic inflammation often connected to allergic responses. Meanwhile STAT6 promotes tumorigenesis in some cancers by regulating genes responsible for cell survival and proliferation. It connects with proteins like IL-4 and IL-13 in these disorders which suggests potential therapeutic targets for treatment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-STAT6 (phospho Y641) antibody (ab28829)
Loading control is Actin
Lanes 1 - 2: STAT6 antibody
Lanes 3 - 4: Western blot - Anti-STAT6 (phospho Y641) antibody (ab28829) at 1/1000 dilution
Lanes 1 and 3: K562 cells treated with 100 ng/mL IL4 at 20 µg
Lanes 2 and 4: K562 untreated cells at 20 µg
SecondaryAll lanes: Dylight 800 secondary antibody at 1/10000 dilution
Predicted band size: 94 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody (ab28829)
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-STAT6 (phospho Y641) antibody (ab28829) at 1/100 dilution.
Western blot - Anti-STAT6 (phospho Y641) antibody (ab28829)
All lanes: Western blot - Anti-STAT6 (phospho Y641) antibody (ab28829)
Predicted band size: 94 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody (ab28829)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling STAT6 (phospho Y641) with ab28829. Right pane - blocked with the phospho peptide.
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody (ab28829)
ab28829 at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum. A heat mediated antigen retrieval step was performed prior to incubation with the antibody. Goat Anti-Rabbit IgG H&L (Biotin) ab6720 (Goat polyclonal to Rabbit IgG H&L (biotin))
was used as the secondary antibody.
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