JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB28829

Anti-STAT6 (phospho Y641) antibody

5

(12 Reviews)

|

(45 Publications)

Rabbit Polyclonal STAT6 phospho Y641 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 45 publications. Immunogen corresponding to Synthetic Peptide within Human STAT6 phospho Y641.

View Alternative Names

Signal transducer and activator of transcription 6, IL-4 Stat, STAT6

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody (AB28829)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody (AB28829)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling STAT6 (phospho Y641) with ab28829. Right pane - blocked with the phospho peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody (AB28829)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody (AB28829)

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-STAT6 (phospho Y641) antibody (ab28829) at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody (AB28829)
  • IHC-P

AbReview7347****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody (AB28829)

ab28829 at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum. A heat mediated antigen retrieval step was performed prior to incubation with the antibody. ab6720 (Goat polyclonal to Rabbit IgG H&L (biotin)) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Western blot - Anti-STAT6 (phospho Y641) antibody (AB28829)
  • WB

Supplier Data

Western blot - Anti-STAT6 (phospho Y641) antibody (AB28829)

Loading control is Actin

Lanes 1 - 2:

STAT6 antibody

Lanes 3 - 4:

Western blot - Anti-STAT6 (phospho Y641) antibody (ab28829) at 1/1000 dilution

Lanes 1 and 3:

K562 cells treated with 100 ng/mL IL4 at 20 µg

Lanes 2 and 4:

K562 untreated cells at 20 µg

Secondary

All lanes:

Dylight 800 secondary antibody at 1/10000 dilution

Predicted band size: 94 kDa

false

Western blot - Anti-STAT6 (phospho Y641) antibody (AB28829)
  • WB

Supplier Data

Western blot - Anti-STAT6 (phospho Y641) antibody (AB28829)

Western blot analysis of lysates from HeLa cells treated with IL-4, using STAT6 (Phospho-Tyr641) Antibody. The lane on the right is blocked with the phospho peptide.

All lanes:

Western blot - Anti-STAT6 (phospho Y641) antibody (ab28829)

Predicted band size: 94 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human STAT6 phospho Y641. The exact immunogen used to generate this antibody is proprietary information.

P42226

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STAT6 also known as Signal Transducer and Activator of Transcription 6 is a transcription factor with a mass of around 94 kDa. This protein is an important component in the signaling pathways that respond to cytokines especially those of the IL-4 and IL-13 receptors. STAT6 mainly expresses in immune cells including T cells B cells and macrophages. It exists in the cytoplasm until the cytokine stimulation prompts its phosphorylation and translocation to the nucleus where it influences gene expression.
Biological function summary

STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.

Pathways

STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.

STAT6 has associations with asthma and certain types of cancer such as colorectal cancer. In asthma STAT6 influences the production of IgE and eosinophilic inflammation often connected to allergic responses. Meanwhile STAT6 promotes tumorigenesis in some cancers by regulating genes responsible for cell survival and proliferation. It connects with proteins like IL-4 and IL-13 in these disorders which suggests potential therapeutic targets for treatment.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Carries out a dual function : signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.
See full target information STAT6 phospho Y641

Publications (45)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113517 PubMed41031375

2025

M1-like macrophages regulate T cell infiltration in colorectal cancer through P2X4 receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Zhou,Xintian Zhang,Yu Liang,Han Yao,Yichao Hou,Xingming Zhang,Leilei Du,Wenfeng Wang,Jianhua Wang,Xiangjun Meng

Cell reports 44:116342 PubMed41004340

2025

Neuropeptide adrenomedullin remodels stemness and macrophage dynamics in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Heba Ali,Fatima Khan,Wenjing Xuan,Yang Liu,Yuyun Huang,Donovan Whitfield,Lizhi Pang,Peiwen Chen

Molecular cancer 24:34 PubMed39876004

2025

Sorafenib enhanced the function of myeloid-derived suppressor cells in hepatocellular carcinoma by facilitating PPARα-mediated fatty acid oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Chunxiao Li,Liting Xiong,Yuhan Yang,Ping Jiang,Junjie Wang,Mengyuan Li,Shuhua Wei,Suqing Tian,Yuexuan Wang,Mi Zhang,Jie Tang

Nature immunology 24:1654-1670 PubMed37667051

2023

Kunitz-type protease inhibitor TFPI2 remodels stemness and immunosuppressive tumor microenvironment in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lizhi Pang,Madeline Dunterman,Songlin Guo,Fatima Khan,Yang Liu,Erfan Taefi,Atousa Bahrami,Changiz Geula,Wen-Hao Hsu,Craig Horbinski,Charles David James,Peiwen Chen

Science advances 8:eabo4363 PubMed35984890

2022

A highly selective JAK3 inhibitor is developed for treating rheumatoid arthritis by suppressing γc cytokine-related JAK-STAT signal.

Applications

Unspecified application

Species

Unspecified reactive species

Chengjuan Chen,Yuan Yin,Gaona Shi,Yu Zhou,Shuai Shao,Yazi Wei,Lei Wu,Dayong Zhang,Lan Sun,Tiantai Zhang

Molecular systems biology 18:e10855 PubMed35959629

2022

Drug-microenvironment perturbations reveal resistance mechanisms and prognostic subgroups in CLL.

Applications

Unspecified application

Species

Unspecified reactive species

Peter-Martin Bruch,Holly Ar Giles,Carolin Kolb,Sophie A Herbst,Tina Becirovic,Tobias Roider,Junyan Lu,Sebastian Scheinost,Lena Wagner,Jennifer Huellein,Ivan Berest,Mark Kriegsmann,Katharina Kriegsmann,Christiane Zgorzelski,Peter Dreger,Judith B Zaugg,Carsten Müller-Tidow,Thorsten Zenz,Wolfgang Huber,Sascha Dietrich

International journal of immunopathology and pharmacology 36:3946320221109529 PubMed35726645

2022

Activation of STAT6 by intranasal allergens correlated with the development of eosinophilic chronic rhinosinusitis in a mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Hongqi Wei,Longjiang Xu,Peng Sun,Hongyu Xing,Zhengwen Zhu,Jisheng Liu

Cellular and molecular gastroenterology and hepatology 13:1449-1467 PubMed35108658

2022

CD73 Epithelial Progenitor Cells That Contribute to Homeostasis and Renewal Are Depleted in Eosinophilic Esophagitis.

Applications

Unspecified application

Species

Unspecified reactive species

Takeo Hara,Yuta Kasagi,Joshua Wang,Masaru Sasaki,Bailey Aaron,Adam Karami,Masataka Shimonosono,Rieko Shimonosono,Hisatsugu Maekawa,Lauren Dolinsky,Benjamin Wilkins,Jeremy Klein,Jane Wei,Kathryn Nunes,Kristle Lynch,Jonathan M Spergel,Kathryn E Hamilton,Melanie A Ruffner,Tatiana A Karakasheva,Kelly A Whelan,Hiroshi Nakagawa,Amanda B Muir

Molecular oncology 16:2214-2234 PubMed34861103

2021

Anti-Chi3L1 antibody suppresses lung tumor growth and metastasis through inhibition of M2 polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Eun Yu,In Jun Yeo,Dong Ju Son,Jaesuk Yun,Sang-Bae Han,Jin Tae Hong

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed34521750

2021

Interleukins 4 and 13 drive lipid abnormalities in skin cells through regulation of sex steroid hormone synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chenlu Zhang,Mahendran Chinnappan,Courtney A Prestwood,Marshall Edwards,Methinee Artami,Bonne M Thompson,Kaitlyn M Eckert,Goncalo Vale,Christos C Zouboulis,Jeffrey G McDonald,Tamia A Harris-Tryon
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com