Skip to main content

Rabbit Recombinant Monoclonal STAT6 phospho Y641 antibody. Suitable for IP, Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Synthetic peptide samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (AB188080), expandable thumbnail
  • Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (AB188080), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (AB188080), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (AB188080), expandable thumbnail
  • Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (AB188080), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPDotWBICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Tested
Tested
Tested
Synthetic peptide
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

p-STAT6 expression is typically undetectable or present at very low levels in healthy tissue lysates (e.g., kidney, lung, and skin) under physiological conditions (PMID: 34831280; PMID: 22401596; PMID: 24955743; PMID: 36629780). However, its expression is markedly upregulated in pathological states, including inflammatory responses and oncogenic activation (PMID: 36884218 ).

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/400
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

Alternative names

Signal transducer and activator of transcription 6, IL-4 Stat, STAT6

Recommended products

Rabbit Recombinant Monoclonal STAT6 phospho Y641 antibody. Suitable for IP, Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Synthetic peptide samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR18278-265
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

STAT6 also known as Signal Transducer and Activator of Transcription 6 is a transcription factor with a mass of around 94 kDa. This protein is an important component in the signaling pathways that respond to cytokines especially those of the IL-4 and IL-13 receptors. STAT6 mainly expresses in immune cells including T cells B cells and macrophages. It exists in the cytoplasm until the cytokine stimulation prompts its phosphorylation and translocation to the nucleus where it influences gene expression.

Biological function summary

STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.

Pathways

STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.

Associated diseases and disorders

STAT6 has associations with asthma and certain types of cancer such as colorectal cancer. In asthma STAT6 influences the production of IgE and eosinophilic inflammation often connected to allergic responses. Meanwhile STAT6 promotes tumorigenesis in some cancers by regulating genes responsible for cell survival and proliferation. It connects with proteins like IL-4 and IL-13 in these disorders which suggests potential therapeutic targets for treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (ab188080), expandable thumbnail

    Western blot - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (ab188080)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Phosphorylation of STAT6 at Y641 can be induced by IL-4 treatment according to the literature (PMID: 21411736, PMID: 15044251, PMID: 14660485).

    This antibody recognizes three isoforms of STAT6, isoform 1 (110KD), isoform 2 (75KD), isoform 3 (81KD), which is consistent with the Uniprot annotation.

    All lanes: Western blot - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (ab188080) at 1/1000 dilution

    Lane 1: Untreated Daudi (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

    Lane 2: Daudi whole cell lysate treated with 100 ng/ml interleukin 4 (IL-4) for 15 minutes at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 94 kDa

    Observed band size: 110 kDa

    Exposure time: 5s

  • Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (ab188080), expandable thumbnail

    Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (ab188080)

    STAT6 (phospho Y641) was immunoprecipitated from 0.35 mg of Daudi (Human Burkitt's lymphoma cell line) whole cell lysate treated with 100 ng/ml IL-4 for 15 minutes with ab188080 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188080 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Daudi whole cell lysate treated with 100 ng/ml IL-4 for 15 minutes, 10μg (Input). Lane 2: ab188080 IP in Daudi whole cell lysate treated with 100 ng/ml IL-4 for 15 minutes. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab188080 in Daudi whole cell lysate treated with 100 ng/ml IL-4 for 15 minutes.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This antibody recognizes three isoforms of STAT6, isoform 1 (110KD), isoform 2 (75KD), isoform 3 (81KD), which is consistent with the Uniprot annotation.

    All lanes: Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (ab188080)

    Predicted band size: 94 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (ab188080), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (ab188080)

    Immunofluorescent analysis of 100% methanol-fixed Daudi (Human Burkitt's lymphoma cell line) cells, treated with interleukin 4 (IL-4) (100 ng/ml) for 15minutes, or untreated, labeling STAT6 (phospho Y641) with ab188080 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    The expression increased and there was nuclear translocation of STAT6 after treatment with interleukin 4 (IL-4) (100 ng/ml) for 15minutes (PMID:22226123).

    The nuclear counterstain is DAPI (blue). Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (ab188080), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (ab188080)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Daudi (Human Burkitt's lymphoma cell line) treated/untreated with 100 ng/ml IL-4 for 15 minutes cells labeling STAT6 (phospho Y641) with ab188080 at 1/400 dilution (treated, red; untreated, green) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (ab188080), expandable thumbnail

    Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (ab188080)

    Dot blot analysis of STAT6 (phospho Y641) labeled with ab188080 at 1/1000 dilution.

    Lane 1: STAT6 (phospho Y641) phospho peptide.

    Lane 2: STAT6 non-phospho peptide.

    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com