AB188080

Anti-STAT6 (phospho Y641) antibody [EPR18278-265]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal STAT6 phospho Y641 antibody. Suitable for IP, Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Synthetic peptide samples. Cited in 2 publications.

View Alternative Names

Signal transducer and activator of transcription 6, IL-4 Stat, STAT6

5 Images

Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (AB188080)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (AB188080)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Daudi (Human Burkitt's lymphoma cell line) treated/untreated with 100 ng/ml IL-4 for 15 minutes cells labeling STAT6 (phospho Y641) with ab188080 at 1/400 dilution (treated, red; untreated, green) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (AB188080)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (AB188080)

Immunofluorescent analysis of 100% methanol-fixed Daudi (Human Burkitt's lymphoma cell line) cells, treated with interleukin 4 (IL-4) (100 ng/ml) for 15minutes, or untreated, labeling STAT6 (phospho Y641) with ab188080 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

The expression increased and there was nuclear translocation of STAT6 after treatment with interleukin 4 (IL-4) (100 ng/ml) for 15minutes (PMID : 22226123).

The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (AB188080)

STAT6 (phospho Y641) was immunoprecipitated from 0.35 mg of Daudi (Human Burkitt's lymphoma cell line) whole cell lysate treated with 100 ng/ml IL-4 for 15 minutes with ab188080 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188080 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Daudi whole cell lysate treated with 100 ng/ml IL-4 for 15 minutes, 10μg (Input). Lane 2 : ab188080 IP in Daudi whole cell lysate treated with 100 ng/ml IL-4 for 15 minutes. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab188080 in Daudi whole cell lysate treated with 100 ng/ml IL-4 for 15 minutes.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

This antibody recognizes three isoforms of STAT6, isoform 1 (110KD), isoform 2 (75KD), isoform 3 (81KD), which is consistent with the Uniprot annotation.

All lanes:

Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (ab188080)

Predicted band size: 94 kDa

false

Supplier Data

Western blot - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (AB188080)

Blocking/Dilution buffer : 5% NFDM/TBST.

Phosphorylation of STAT6 at Y641 can be induced by IL-4 treatment according to the literature (PMID : 21411736, PMID : 15044251, PMID : 14660485).

This antibody recognizes three isoforms of STAT6, isoform 1 (110KD), isoform 2 (75KD), isoform 3 (81KD), which is consistent with the Uniprot annotation.

All lanes:

Western blot - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (ab188080) at 1/1000 dilution

Lane 1:

Untreated Daudi (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Lane 2:

Daudi whole cell lysate treated with 100 ng/ml interleukin 4 (IL-4) for 15 minutes at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 94 kDa

Observed band size: 110 kDa

false

Exposure time: 5s

Supplier Data

Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (AB188080)

Dot blot analysis of STAT6 (phospho Y641) labeled with ab188080 at 1/1000 dilution.

Lane 1 : STAT6 (phospho Y641) phospho peptide.

Lane 2 : STAT6 non-phospho peptide.

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution was used as secondary antibody.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Carrier free

Anti-STAT6 (phospho Y641) antibody [EPR18278-265] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18278-265

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Dot, Flow Cyt (Intra), ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "p-STAT6 expression is typically undetectable or present at very low levels in healthy tissue lysates (e.g., kidney, lung, and skin) under physiological conditions (PMID: 34831280; PMID: 22401596; PMID: 24955743; PMID: 36629780). However, its expression is markedly upregulated in pathological states, including inflammatory responses and oncogenic activation (PMID: 36884218 ).", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/400", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STAT6 also known as Signal Transducer and Activator of Transcription 6 is a transcription factor with a mass of around 94 kDa. This protein is an important component in the signaling pathways that respond to cytokines especially those of the IL-4 and IL-13 receptors. STAT6 mainly expresses in immune cells including T cells B cells and macrophages. It exists in the cytoplasm until the cytokine stimulation prompts its phosphorylation and translocation to the nucleus where it influences gene expression.

Biological function summary

STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.

Pathways

STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.

STAT6 has associations with asthma and certain types of cancer such as colorectal cancer. In asthma STAT6 influences the production of IgE and eosinophilic inflammation often connected to allergic responses. Meanwhile STAT6 promotes tumorigenesis in some cancers by regulating genes responsible for cell survival and proliferation. It connects with proteins like IL-4 and IL-13 in these disorders which suggests potential therapeutic targets for treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Carries out a dual function : signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

See full target information STAT6 phospho Y641

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 42:e113155 PubMed37886905

2023

A conserved complex of microneme proteins mediates rhoptry discharge in Toxoplasma.

Applications

Unspecified application

Species

Unspecified reactive species

Dylan Valleau,Saima M Sidik,Luiz C Godoy,Yamilex Acevedo-Sánchez,Charisse Flerida A Pasaje,My-Hang Huynh,Vern B Carruthers,Jacquin C Niles,Sebastian Lourido

Cell death & disease 14:134 PubMed36797258

2023

Single cell epigenomic and transcriptomic analysis uncovers potential transcription factors regulating mitotic/meiotic switch.

Applications

Unspecified application

Species

Unspecified reactive species

Fa-Li Zhang,Yan-Qin Feng,Jing-Ya Wang,Ke-Xin Zhu,Lu Wang,Jia-Mao Yan,Xiu-Xiu Li,Jun-Jie Wang,Wei Ge,Massimo De Felici,Wei Shen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-STAT6 (phospho Y641) antibody [EPR18278-265]

Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (AB188080)

Immunocytochemistry/ Immunofluorescence - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (AB188080)

Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (AB188080)

Western blot - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (AB188080)

Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR18278-265] (AB188080)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise