AB263949

Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal STAT6 phospho Y641 antibody. Carrier free. Suitable for Flow Cyt (Intra), IP, ChIP, Dot, WB and reacts with Human, Mouse, Synthetic peptide, Rat samples. Cited in 1 publication.

View Alternative Names

Signal transducer and transcription activator 6, Stat6

6 Images

Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Daudi (Human Burkitt's lymphoma lymphoblast) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min (Red) / Untreated control (Green) cells labelling STAT6 (phospho Y641) with ab235591 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235591).

Unknown

Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)

STAT6 (phospho Y641) was immunoprecipitated from 0.35 mg Daudi (Human Burkitt's lymphoma lymphoblast) cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate, with ab235591 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab235591 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Daudi cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug

Lane 2 : ab235591 IP in Daudi was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab235591 in Daudi cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235591).

All lanes:

Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (<a href='/en-us/products/primary-antibodies/stat6-phospho-y641-antibody-epr22599-52-chip-grade-ab235591'>ab235591</a>)

Predicted band size: 94 kDa

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ChIP - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)

ChIP

Unknown

ChIP - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)

Chromatin was prepared from Daudi cells starved for 24h, then treated with hIL-4(100ng/ml, 15min) according to the Abcam Dual-X-ChIP protocol. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 μg of chromatin, 5 μg of ab235591 (red), or 5 μg of rabbit normal IgG ab172730 (gray) and 20 μl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
Primers and probes are commercial primers from Cell Signaling Technology (Cat. No. : #56554)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235591).

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min (Red) / Untreated control (Green) cells labelling STAT6 (phospho Y641) with ab235591 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235591).

Unknown

Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)

STAT6 (phospho Y641) was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate with ab235591 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab235591 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : RAW 264.7 cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug

Lane 2 : ab235591 IP in RAW 264.7 cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab235591 in RAW 264.7 was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235591).

All lanes:

Predicted band size: 94 kDa

false

Unknown

Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)

Dot blot analysis using ab235591 at 1/1000 dilution, Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at 1/100,000 diluton.

Lane 1 : STAT6 (phospho Y641) peptide (aa636-647).

Lane 2 : STAT6 non-phospho peptide (aa636-647)

Exposure time : 26 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235591).

Unconjugated

Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade
660 APC

APC Anti-STAT6 (phospho Y641) antibody [EPR22599-52] (ChIP Grade)
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade
578 PE

PE Anti-STAT6 (phospho Y641) antibody [EPR22599-52] (ChIP Grade)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22599-52

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ChIP, WB, Flow Cyt (Intra), Dot, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "p-STAT6 expression is typically undetectable or present at very low levels in healthy tissue lysates (e.g., kidney, lung, and skin) under physiological conditions (PMID: 34831280; PMID: 22401596; PMID: 24955743; PMID: 36629780). However, its expression is markedly upregulated in pathological states, including inflammatory responses and oncogenic activation (PMID: 36884218 ).", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "p-STAT6 expression is typically undetectable or present at very low levels in healthy tissue lysates (e.g., kidney, lung, and skin) under physiological conditions (PMID: 34831280; PMID: 22401596; PMID: 24955743; PMID: 36629780). However, its expression is markedly upregulated in pathological states, including inflammatory responses and oncogenic activation (PMID: 36884218 ).", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "p-STAT6 expression is typically undetectable or present at very low levels in healthy tissue lysates (e.g., kidney, lung, and skin) under physiological conditions (PMID: 34831280; PMID: 22401596; PMID: 24955743; PMID: 36629780). However, its expression is markedly upregulated in pathological states, including inflammatory responses and oncogenic activation (PMID: 36884218 ).", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab263949 is the carrier-free version of ab235591.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

STAT6 also known as Signal Transducer and Activator of Transcription 6 is a transcription factor with a mass of around 94 kDa. This protein is an important component in the signaling pathways that respond to cytokines especially those of the IL-4 and IL-13 receptors. STAT6 mainly expresses in immune cells including T cells B cells and macrophages. It exists in the cytoplasm until the cytokine stimulation prompts its phosphorylation and translocation to the nucleus where it influences gene expression.

Biological function summary

STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.

Pathways

STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.

STAT6 has associations with asthma and certain types of cancer such as colorectal cancer. In asthma STAT6 influences the production of IgE and eosinophilic inflammation often connected to allergic responses. Meanwhile STAT6 promotes tumorigenesis in some cancers by regulating genes responsible for cell survival and proliferation. It connects with proteins like IL-4 and IL-13 in these disorders which suggests potential therapeutic targets for treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Carries out a dual function : signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

See full target information Stat6 pY641

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 30:289-300 PubMed37303493

2023

LSM12 facilitates the progression of colorectal cancer by activating the WNT/CTNNB1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhuang,Chunlan Ning,Pengfei Liu,Yanpeng Zhao,Yue Li,Zhenchi Ma,Luling Shan,Yingzhe Piao,Peng Zhao,Xun Jin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)

Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)

ChIP - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)

Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)

Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)

Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise