Rabbit Recombinant Monoclonal STAT6 phospho Y641 antibody. Carrier free. Suitable for Flow Cyt (Intra), IP, ChIP, Dot, WB and reacts with Human, Mouse, Synthetic peptide, Rat samples.
Images
![ChIP - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949), expandable thumbnail](https://content.abcam.com/products/images/stat6-phospho-y641-antibody-epr22599-52-bsa-and-azide-free-ab263949--chip-img120675.jpg/_jcr_content/renditions/webp-475.webp "ChIP - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)")
![Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949), expandable thumbnail](https://content.abcam.com/products/images/stat6-phospho-y641-antibody-epr22599-52-bsa-and-azide-free-ab263949--immunoprecipitation-img137133.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)")
![Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949), expandable thumbnail](https://content.abcam.com/products/images/stat6-phospho-y641-antibody-epr22599-52-bsa-and-azide-free-ab263949--immunoprecipitation-img8654.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)")
![Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949), expandable thumbnail](https://content.abcam.com/products/images/stat6-phospho-y641-antibody-epr22599-52-bsa-and-azide-free-ab263949--dot-blot-img4836.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)")
![Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949), expandable thumbnail](https://content.abcam.com/products/images/stat6-phospho-y641-antibody-epr22599-52-bsa-and-azide-free-ab263949--flow-cytometry-intracellular-img27205.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (AB263949)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | IP | ChIP | Dot | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected | Expected | Not recommended | Not recommended |
| Mouse | Tested | Tested | Expected | Expected | Expected | Not recommended | Not recommended |
| Rat | Predicted | Predicted | Predicted | Predicted | Expected | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
4 products for Alternative Version
Target data
Function
Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.
Alternative names
Signal transducer and transcription activator 6, Stat6
Recommended products
Rabbit Recombinant Monoclonal STAT6 phospho Y641 antibody. Carrier free. Suitable for Flow Cyt (Intra), IP, ChIP, Dot, WB and reacts with Human, Mouse, Synthetic peptide, Rat samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR22599-52
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab263949 is the carrier-free version of Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
STAT6 also known as Signal Transducer and Activator of Transcription 6 is a transcription factor with a mass of around 94 kDa. This protein is an important component in the signaling pathways that respond to cytokines especially those of the IL-4 and IL-13 receptors. STAT6 mainly expresses in immune cells including T cells B cells and macrophages. It exists in the cytoplasm until the cytokine stimulation prompts its phosphorylation and translocation to the nucleus where it influences gene expression.
- Biological function summary
STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.
- Pathways
STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.
- Associated diseases and disorders
STAT6 has associations with asthma and certain types of cancer such as colorectal cancer. In asthma STAT6 influences the production of IgE and eosinophilic inflammation often connected to allergic responses. Meanwhile STAT6 promotes tumorigenesis in some cancers by regulating genes responsible for cell survival and proliferation. It connects with proteins like IL-4 and IL-13 in these disorders which suggests potential therapeutic targets for treatment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![ChIP - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949), expandable thumbnail](https://content.abcam.com/products/images/stat6-phospho-y641-antibody-epr22599-52-bsa-and-azide-free-ab263949--chip-img120675.jpg "ChIP - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)")
ChIP - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)
Chromatin was prepared from Daudi cells starved for 24h, then treated with hIL-4(100ng/ml, 15min) according to the Abcam Dual-X-ChIP protocol. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 μg of chromatin, 5 μg of Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591 (red), or 5 μg of rabbit normal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (gray) and 20 μl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
Primers and probes are commercial primers from Cell Signaling Technology (Cat. No.: #56554)This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591).
![Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949), expandable thumbnail](https://content.abcam.com/products/images/stat6-phospho-y641-antibody-epr22599-52-bsa-and-azide-free-ab263949--immunoprecipitation-img137133.jpg "Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)")
Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)
STAT6 (phospho Y641) was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate with Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: RAW 264.7 cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug
Lane 2: Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591 IP in RAW 264.7 cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591 in RAW 264.7 was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591).
All lanes: Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591)
Predicted band size: 94 kDa
![Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949), expandable thumbnail](https://content.abcam.com/products/images/stat6-phospho-y641-antibody-epr22599-52-bsa-and-azide-free-ab263949--immunoprecipitation-img8654.jpg "Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)")
Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)
STAT6 (phospho Y641) was immunoprecipitated from 0.35 mg Daudi (Human Burkitt's lymphoma lymphoblast) cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate, with Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Daudi cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug
Lane 2: Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591 IP in Daudi was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591 in Daudi cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591).
All lanes: Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591)
Predicted band size: 94 kDa
![Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949), expandable thumbnail](https://content.abcam.com/products/images/stat6-phospho-y641-antibody-epr22599-52-bsa-and-azide-free-ab263949--dot-blot-img4836.jpg "Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)")
Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)
Dot blot analysis using Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591 at 1/1000 dilution, Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at 1/100,000 diluton.
Lane 1: STAT6 (phospho Y641) peptide (aa636-647).
Lane 2: STAT6 non-phospho peptide (aa636-647)
Exposure time: 26 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591).
![Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949), expandable thumbnail](https://content.abcam.com/products/images/stat6-phospho-y641-antibody-epr22599-52-bsa-and-azide-free-ab263949--flow-cytometry-intracellular-img27205.jpg "Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)")
Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min (Red) / Untreated control (Green) cells labelling STAT6 (phospho Y641) with Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591).
![Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949), expandable thumbnail](https://content.abcam.com/products/images/stat6-phospho-y641-antibody-epr22599-52-bsa-and-azide-free-ab263949--flow-cytometry-intracellular-img95613.jpg "Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)")
Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Daudi (Human Burkitt's lymphoma lymphoblast) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min (Red) / Untreated control (Green) cells labelling STAT6 (phospho Y641) with Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591 at 1/500 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade ab235591).
![Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949), expandable thumbnail](https://content.abcam.com/products/images/stat6-phospho-y641-antibody-epr22599-52-bsa-and-azide-free-ab263949--flow-cytometry-intracellular-img95613.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)")
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