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Rabbit Recombinant Monoclonal STAT6 phospho Y641 antibody. Suitable for IP, ChIP, Dot, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat, Synthetic peptide - Mouse, Synthetic peptide - Rat, Synthetic peptide - Human samples.

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Images

ChIP - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (AB235591), expandable thumbnail
  • Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (AB235591), expandable thumbnail
  • Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (AB235591), expandable thumbnail
  • Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (AB235591), expandable thumbnail
  • Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (AB235591), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPChIPDotWBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Expected
Tested
Not recommended
Not recommended
Tested
Mouse
Tested
Expected
Expected
Tested
Not recommended
Not recommended
Tested
Rat
Expected
Expected
Expected
Tested
Not recommended
Not recommended
Expected
Synthetic peptide - Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Synthetic peptide - Mouse
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Synthetic peptide - Rat
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Mouse, Synthetic peptide - Rat, Synthetic peptide - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Mouse, Synthetic peptide - Rat, Synthetic peptide - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide - Mouse

Dilution info

-

Notes

-

Species

Synthetic peptide - Rat

Dilution info

1/1000

Notes

-

Species

Synthetic peptide - Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Mouse, Human, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Mouse, Synthetic peptide - Rat, Synthetic peptide - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human, Synthetic peptide - Mouse, Synthetic peptide - Rat, Synthetic peptide - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat, Synthetic peptide - Mouse, Synthetic peptide - Rat, Synthetic peptide - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Mouse, Synthetic peptide - Rat, Synthetic peptide - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

Alternative names

Signal transducer and transcription activator 6, Stat6

Recommended products

Rabbit Recombinant Monoclonal STAT6 phospho Y641 antibody. Suitable for IP, ChIP, Dot, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat, Synthetic peptide - Mouse, Synthetic peptide - Rat, Synthetic peptide - Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22599-52

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

STAT6 also known as Signal Transducer and Activator of Transcription 6 is a transcription factor with a mass of around 94 kDa. This protein is an important component in the signaling pathways that respond to cytokines especially those of the IL-4 and IL-13 receptors. STAT6 mainly expresses in immune cells including T cells B cells and macrophages. It exists in the cytoplasm until the cytokine stimulation prompts its phosphorylation and translocation to the nucleus where it influences gene expression.

Biological function summary

STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.

Pathways

STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.

Associated diseases and disorders

STAT6 has associations with asthma and certain types of cancer such as colorectal cancer. In asthma STAT6 influences the production of IgE and eosinophilic inflammation often connected to allergic responses. Meanwhile STAT6 promotes tumorigenesis in some cancers by regulating genes responsible for cell survival and proliferation. It connects with proteins like IL-4 and IL-13 in these disorders which suggests potential therapeutic targets for treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • ChIP - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591), expandable thumbnail

    ChIP - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591)

    Chromatin was prepared from Daudi cells starved for 24h, then treated with hIL-4(100ng/ml, 15min) according to the Abcam Dual-X-ChIP protocol. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
    The ChIP was performed with 25 μg of chromatin, 5 μg of ab235591 (red), or 5 μg of rabbit normal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (gray) and 20 μl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
    Primers and probes are commercial primers from Cell Signaling Technology (Cat. No.: #56554)

  • Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591), expandable thumbnail

    Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST Phosphorylation of STAT6 at Y641 can be induced by IL-4 treatment (PMID: 21411736,15044251) The molecular weight observed is consistent with what has been described in the literature (PMID: 24708771) Exposure time: 4 seconds

    All lanes: Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591) at 1/1000 dilution

    Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) serum starved for 24 hours, whole cell lysate at 10 µg

    Lane 2: RAW264.7 serum starved for 24 hours, then treated with 100 ng/ml mIL-4 for 15 minutes, whole cell lysate 10 ug

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 94 kDa

    Observed band size: 110 kDa, 94 kDa

  • Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591), expandable thumbnail

    Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST Phosphorylation of STAT6 at Y641 can be induced by IL-4 treatment (PMID: 21411736,15044251) The molecular weight observed is consistent with what has been described in the literature (PMID: 24708771) Exposure time: 15 seconds

    All lanes: Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591) at 1/1000 dilution

    Lane 1: 2.4G2 (rat B cell lymphoma B lymphocyte) serum starved for 24 hours, whole cell lysate at 10 µg

    Lane 2: 2.4G2 serum starved for 24 hours, then treated with 100 ng/ml rIL-4 for 15 minutes, whole cell lysate 10 ug

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 94 kDa

    Observed band size: 110 kDa, 94 kDa

  • Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591), expandable thumbnail

    Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591)

    STAT6 (phospho Y641) was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate with ab235591 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab235591 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: RAW 264.7 cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug

    Lane 2: ab235591 IP in RAW 264.7 cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab235591 in RAW 264.7 was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    All lanes: Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591)

    Predicted band size: 94 kDa

  • Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591), expandable thumbnail

    Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST Phosphorylation of STAT6 at Y641 can be induced by IL-4 treatment (PMID: 21411736,15044251) This antibody recognizes three isoforms of STAT6, isoform 1 (110 kDa), isoform 2 (75 kDa), isoform 3 (81 k Da), which is consistent with the Uniprot annotation. Exposure time: 4 seconds

    All lanes: Western blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591) at 1/1000 dilution

    Lane 1: Daudi (human Burkitts lymphoma lymphoblast) serum starved for 24 hours, whole cell lysate at 10 µg

    Lane 2: Daudi serum starved for 24 hours, then treated with 100 ng/ml IL-4 for 15 minutes, whole cell lysate 10 ug

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 94 kDa

    Observed band size: 110 kDa, 75 kDa, 81 kDa

  • Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591), expandable thumbnail

    Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591)

    STAT6 (phospho Y641) was immunoprecipitated from 0.35 mg Daudi (Human Burkitt's lymphoma lymphoblast) cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate, with ab235591 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab235591 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Daudi cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug

    Lane 2: ab235591 IP in Daudi was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab235591 in Daudi cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591)

    Predicted band size: 94 kDa

  • Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591), expandable thumbnail

    Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591)

    Dot blot analysis using ab235591 at 1/1000 dilution, Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at 1/100,000 diluton.

    Lane 1: STAT6 (phospho Y641) peptide (aa636-647).

    Lane 2: STAT6 non-phospho peptide (aa636-647)

    Exposure time: 26 seconds.

  • Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min (Red) / Untreated control (Green) cells labelling STAT6 (phospho Y641) with ab235591 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - ChIP Grade (ab235591)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Daudi (Human Burkitt's lymphoma lymphoblast) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min (Red) / Untreated control (Green) cells labelling STAT6 (phospho Y641) with ab235591 at 1/500 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

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